Question (74941) | Anti-Firefly Luciferase antibody [Luci17] (ab16466)

Go to datasheet (ab16466)

Question

Primary antibody dilutions are not specified either in the website or in the paper that used this antibody for flow cytometry and are cited in the website.

Could you give me this information so I can have a starting point?

Answer

The flow cytometry image shown on ab16466's datasheet is from: Pellegatti P et al. Increased level of extracellular ATP at tumor sites: in vivo imaging with plasma membrane luciferase. PLoS ONE 3:e2599 (2008) as stated in the figure legend.

From the materials section of this paper, it states that cells were incubated with ab16466 (8–10 µg/5×10^5 cells.) I would therefore use this as a starting point for your experiment.