FirePlex®-384 Kidney Toxicity (Human) Immunoassay Panel (ab256464)
Overview
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Product name
FirePlex®-384 Kidney Toxicity (Human) Immunoassay Panel -
Detection method
Fluorescent -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Multiplex -
Species reactivity
Reacts with: Human -
Product overview
FirePlex®-384 Kidney Toxicity (Human) Immunoassay Panel (ab256464) uses FirePlex® particle technology to quantitate KIM-1, Clusterin, Cistatin-c, Lipocalin-2, Albumin, Osteopontin from 6.25 µl of sample input in 384-well plate format.
Assays are validated in serum, plasma, and cell culture supernatant, and can be run with 175 samples in duplicate per 384-well plate.
The two-step workflow and no-wash assay format limit hands-on time and are amenable to automation, thus making FirePlex®-384 ideally suited for high-throughput screening studies. Assay readout is conducted on high-content imagers with data analysis using the FirePlex® Analysis Workbench software.
Users should note that assay performance data supplied in the accompanying Product Datasheet is a representative dataset, and is not lot-specific. For lot-specific assay performance data, please refer to the product's certificate of analysis, which can be found here.
FirePlex® is a registered trade mark in certain territories (including Europe and the United States) and is an unregistered trade mark elsewhere.
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Notes
This product is currently only supported for customers in North America and Europe, if you are outside these areas please contact us.
The following table provides the sensitivity, dynamic range, and CVs for FirePlex®-384 Kidney Toxicity (Human) Immunoassay Panel*.
Target Sensitivity/LOD
(pg/mL)
Dynamic Range
(pg/mL)
Inter-well CV
(Std curve)
Clusterin 77.54 123.46 - 30,000 7.18% Cystatin-C 917.84 1,111.11 - 90,000 5.90% KIM1 331.92 370.37 - 90,000 8.90% Lipocalin-2 57.8 123.46 - 10,000 9.58% Osteopontin 139.84 370.37 - 30,000 9.31% Serum Albumin 58.96 123.46 - 30,000 10.40% *representative dataset, not lot-specific. For lot-specific assay performance data, please refer to the product's certificate of analysis, which can be found here.
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Platform
Multiplex
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 384 tests 384 Well Imaging Plate 1 unit 384 Well Imaging Plate Lid 1 unit 384 Well Imaging Plate Seal 2 units FirePlex-384 - Imaging Dye Concentrate 1 x 875µl FirePlex-384 1X PBS 1 x 8ml FirePlex-384 35X Detector Antibody Mix 1 x 110µl FirePlex-384 5X Capture Particle Mix 1 x 700µl FirePlex-384 Reagent Diluent R1 1 x 8ml FirePlex-384 Sample Diluent S1 1 x 25ml FirePlex-HT Human Protein Standard Mix HTKTX 1 vial -
Research areas
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Cellular localization
Clusterin: Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis. Albumin: Secreted. Osteopontin: Secreted. Cystatin C: Secreted. TIM 1: Membrane. Lipocalin-2 / NGAL: Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. -
Database links
- Entrez Gene: 1191 Human
- Entrez Gene: 1471 Human
- Entrez Gene: 213 Human
- Entrez Gene: 26762 Human
- Entrez Gene: 3934 Human
- Entrez Gene: 6696 Human
- Omim: 103600 Human
- Omim: 166490 Human
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Images
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Standard curve
ab256464 standard curves.
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Assay WorkflowAssay Workflow
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab256464 has not yet been referenced specifically in any publications.