Overview

  • Product name
    FirePlex Human Inflammation - Immunoassay Panel
  • Detection method
    Fluorescent
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Other biological fluids, Heparin Plasma, EDTA Plasma, Citrate Plasma, Cerebral Spinal Fluid
  • Assay type
    Multiplex
  • Assay time
    3h 30m
  • Species reactivity
    Reacts with: Human
  • Product overview

    FirePlex Human Inflammation - Immunoassay Panel (ab229793) uses the FirePlex® particle technology to quantify up to 8 human proteins and peptides in the same well, from 12.5 µL sample input.


    Assay run-time is 3.5 hours, followed by particle analysis using a validated flow cytometer model and data analysis using our integrated, free-of-charge FirePlex Analysis Workbench software.
    https://www.abcam.com/kits/multiplex-immunoassays-firefly-analysis-workbench-software


    FirePlex Human Inflammation - Immunoassay Panel (ab229793) requires the purchase of
    - A FirePlex Immunoassay Human Core Reagent Kit (ab208203)
    - and FirePlex Immunoassay Protein Standard Mix A (ab227934)


    FirePlex® is a registered trade mark in the United States and is registered as a European Union Trade Mark.

  • Notes

    This panel was previously available under the product code ab213391, which is discontinued. We have redeveloped this panel using new, recombinant monoclonal antibodies that provide higher sensitivity and better reproducibility when quantifying the analytes with biofluid samples, improving consistency in kit performance.

    The following information provides the sensitivity, range, CVs, required Protein Standard Mix and links to the individual target kit, where more information regarding recommeneded dilutions can be found. 

    Target

    Sensitivity
    (pg/mL)
    Dynamic Range
    (pg/mL)
    Particle Code Protein
    Standard
    TNF alpha  5.36 4.57 - 10,000 Mix A
     IFN gamma 2.25 4.57 - 10,000 23  Mix A
    IL-1 beta 0.47 1.52 - 3,333 21  Mix A
    IL-6 0.15 0.51 - 1,111 9 Mix A
    IL-8 0.29 0.51 - 1,111 11  Mix A
    IL-10 1.12 4.57 - 10,000 13  Mix A
    IL-12p70  0.64 4.57 - 10,000 25  Mix A
    MCP1 0.28 1.52 - 3,333 31 

    Mix A

     

  • Platform
    Multiplex

Properties

Images

  • Mean background-subtracted data values are graphed (+/- SD).

Protocols

References

ab229793 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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