FirePlex Human Inflammation - Immunoassay Panel (ab229793)

Overview

  • Product name
    FirePlex Human Inflammation - Immunoassay Panel
  • Detection method
    Flow cytometry-fluorescent
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Species reactivity
    Reacts with: Human
  • Product overview

    Our multiplex immunoassays use the FirePlex® particle technology to enable the quantitative measurement of many human proteins and peptides at the same time using as little as 12.5 μL of sample per well.
    Assay run-time is 3.5 hours, followed by particle analysis using a validated flow cytometer model and data analysis using our integrated, free-of-charge FirePlex Analysis Workbench software.


    FirePlex immunoassays offer:
    - Measurement of multiple analytes in the same well, thus conserving time and precious samples.
    - Flexibility to select either from our catalogue of pre-designed panels or build custom panels from our large antibody pairs portfolio.
    - Antibody pairs that are validated across a broad set of biological sample types, and provide sensitive and reproducible quantitation of analytes in a given sample.


    Find out more about FirePlex particle-based multiplex immunoassays


    Important - Use of the FirePlex Human Inflammation - Immunoassay Panel requires the purchase of:
    - a) A FirePlex Immunoassay Human Core Reagent Kit (ab208203); and
    - b) Human Immunoassay Protein Standard Mix A (ab227934).


    FirePlex® is a registered trade mark in the United States and is registered as a European Union Trade Mark.

  • Notes

    The following information provides the sensitivity, range, CVs, required Protein Standard Mix and links to the individual target kit, where more information regarding recommeneded dilutions can be found. 

    Target

    Sensitivity
    (pg/mL)
    Dynamic Range
    (pg/mL)
    Particle Code Protein
    Standard
    TNF alpha  5.36 4.57 - 10,000 Mix A
     IFN gamma 2.25 4.57 - 10,000 23  Mix A
    IL-1 beta 0.47 1.52 - 3,333 21  Mix A
    IL-6 0.15 0.51 - 1,111 9 Mix A
    IL-8 0.29 0.51 - 1,111 11  Mix A
    IL-10 1.12 4.57 - 10,000 13  Mix A
    IL-12p70  0.64 4.57 - 10,000 25  Mix A
    MCP1 0.28 1.52 - 3,333 31 

    Mix A

     

Properties

Images

  • Mean background-subtracted data values are graphed (+/- SD).

Protocols

References

ab229793 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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