Overview

  • Product name

    FirePlex Human Key Cytokines - Immunoassay Panel
  • Detection method

    Flow cytometry-fluorescent
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Other biological fluids, Hep Plasma, EDTA Plasma, Cit plasma, Cerebral Spinal Fluid
  • Assay type

    Multiplex
  • Assay time

    3h 30m
  • Species reactivity

    Reacts with: Human
  • Product overview

    FirePlex Human Key Cytokines - Immunoassay Panel (ab243549) contains all the reagents you need to run a 17-plex assay: antibody conjugated particles, Human Core Reagents and Protein Standard Mix.


    NOTE: FirePlex Immunoassay panels require the purchase of a Run Buffer. Use our FirePlex Cytometer Setup Kit V2 (ab245835) to select the most appropriate Run Buffer for your flow cytometer.


    Assay run-time is 3.5 hours, followed by particle analysis using a validated flow cytometer model and data analysis using our integrated, free-of-charge FirePlex Analysis Workbench software.


    FirePlex® is a registered trade mark in the United States and is registered as a European Union Trade Mark.

  • Notes

    This panel was previously available under the product code ab231392/ab229791, which is discontinued. We have redeveloped this panel using new, recombinant monoclonal antibodies that provide higher sensitivity and better reproducibility when quantifying the analytes with biofluid samples, improving consistency in kit performance.

    The following information provides the sensitivity, range, CVs, and required Protein Standard Mix.

    Target

    Sensitivity
    (pg/mL)

    Dynamic Range
    (pg/mL)

    Protein 
    Standard

    TNF alpha 

    6.64

    4.57 - 10,000

    Mix A

     IFN gamma

    3.49

    4.57 - 10,000

    Mix A

    IL-1 beta

    0.35

    1.52 - 3,333

    Mix A

    IL-2 

    0.79

    4.57 - 10,000

    Mix A

    IL-4

    0.54

    1.52 - 3,333

    Mix A

    IL-5

    0.11

    0.51 - 1,111

    Mix A

    IL-6

    0.12

    0.51 - 1,111

    Mix A

    IL-8

    0.19

    0.51 - 1,111

    Mix A

    IL-9

    1.32

    4.57 - 10,000

    Mix A

    IL-10

    0.96

    4.57 - 10,000

    Mix A

    IL-12p70 

    0.66

    4.57 - 10,000

    Mix A

    IL-13

    2.56

    4.57 - 10,000

    Mix A

    IL-17A

    0.34

    1.52 - 3,333

    Mix A

    GM-CSF

    0.62

    4.57 - 10,000

    Mix A

    MCP1

    0.54

    1.52 - 3,333

    Mix A

    MIP1a

    0.08

    0.51 - 1,111

    Mix A

    MIP1b

    0.09

    0.51 - 1,111

    Mix A

    Negative Control Particles

    N/A

    N/A

     N/A
  • Platform

    Multiplex

Properties

Images

  • Mean background-subtracted data values are graphed (+/- SD).

Protocols

References

ab243549 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab243549.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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