Key features and details
- Assay type: Multiplex
- Detection method: Flow cytometry-fluorescent
- Platform: Multiplex
- Assay time: 4 hr
Product nameFirePlex® miRNA Panel – Immunology V2
Detection methodFlow cytometry-fluorescent
Assay time4h 00m
Species reactivityReacts with: Mouse, Rat, Human
Multiplex miRNA Assay - Immunology Panel (ab218369) are the FirePlex® particles designed to profile up to 65 literature curated miRNA for immunology studies, miRNAs differentially regulated in normal or abnormal immunological health.
NOTE: FirePlex miRNA panels require the purchase of a Run Buffer. Use our FirePlex Cytometer Setup Kit V2 (ab245835) to select the most appropriate Run Buffer for your flow cytometer.
Our multiplex miRNA assays use our FirePlex® particle technology with the same sensitivity as PCR, they can be used either directly with 10 µL of biofluids such as serum or plasma with no need for RNA purification; or with 100 pg of purified RNA.
Find a full list of validated sample types, and learn more about the performance and requirements of these assays, here.
These miRNAs have been chosen based on a detailed study of data in peer-reviewed journals and exhibit differential regulation in whole blood, plasma, serum, peripheral blood mononuclear cells (PBMCs) or isolated exosomes.
Included in the panel are general markers for multiple immunology-related diseases, markers specific to specific autoimmune diseases and blood-based cancers, markers that may indicate hemolysis contamination in isolated plasma/serum/exosomes and internal controls to validate assay performance.
It is important to use the normalization procedure in the FirePlex® Analysis Workbench Software to identify the miRNAs that can optimally be used for normalization with your samples. Three miRNAs that are likely to be stably expressed in biofluid samples and may be suitable have been included in this panel.
FirePlex® is a registered trade mark in the United States and is an unregistered trade mark elsewhere.
** Hu = Human, Ms = Mouse, Rt = Rat, Ath = Arabidopsis thaliana, Oan = Ornithorhynchus anatinus, Cel = Caenorhabditis elegans.
# miRNA 21 name miRNA Sequence (miRBase V21) Sequence homology ** MIMAT ID 1 hsa-let-7b-5p ugagguaguagguugugugguu Hu, Ms, Rt MIMAT0000063 2 hsa-let-7d-5p agagguaguagguugcauaguu Hu, Ms, Rt MIMAT0000065 3 ath-mir167d ugaagcugccagcaugaucugg Ath MIMAT0000905 4 hsa-let-7g-5p ugagguaguaguuuguacaguu Hu, Ms, Rt MIMAT0000414 5 hsa-let-7i-5p ugagguaguaguuugugcuguu Hu, Ms, Rt MIMAT0000415 6 hsa-miR-10a-5p uacccuguagauccgaauuugug Hu, Ms, Rt MIMAT0000253 7 hsa-miR-122-5p uggagugugacaaugguguuug Hu, Ms, Rt MIMAT0000421 8 hsa-miR-1246 aauggauuuuuggagcagg Hu MIMAT0005898 9 hsa-miR-125a-5p ucccugagacccuuuaaccuguga Hu, Ms, Rt MIMAT0000443 10 hsa-miR-126-3p ucguaccgugaguaauaaugcg Hu, Ms, Rt MIMAT0000445 11 hsa-miR-129-5p cuuuuugcggucugggcuugc Hu, Ms, Rt MIMAT0000242 12 hsa-miR-130a-3p cagugcaauguuaaaagggcau Hu, Ms, Rt MIMAT0000425 13 hsa-miR-132-3p uaacagucuacagccauggucg Hu, Ms, Rt MIMAT0000426 14 hsa-miR-140-3p uaccacaggguagaaccacgg Hu, Ms, Rt MIMAT0004597 15 hsa-miR-142-3p uguaguguuuccuacuuuaugga Hu, Ms, Rt MIMAT0000434 16 hsa-miR-145-5p guccaguuuucccaggaaucccu Hu, Ms, Rt MIMAT0000437 17 hsa-miR-146a-5p ugagaacugaauuccauggguu Hu, Ms, Rt MIMAT0000449 18 hsa-miR-150-5p ucucccaacccuuguaccagug Hu, Ms, Rt MIMAT0000451 19 hsa-miR-151a-5p ucgaggagcucacagucuagu Hu, Ms, Rt MIMAT0004697 20 hsa-miR-154-3p aaucauacacgguugaccuauu Hu, Ms, Rt MIMAT0000453 21 hsa-miR-155-5p uuaaugcuaaucgugauaggggu Hu MIMAT0000646 22 hsa-miR-15a-5p uagcagcacauaaugguuugug Hu, Ms MIMAT0000068 23 hsa-miR-15b-5p uagcagcacaucaugguuuaca Hu, Ms, Rt MIMAT0000417 24 hsa-miR-16-5p uagcagcacguaaauauuggcg Hu, Ms, Rt MIMAT0000069 25 cel-mir-70-3p uaauacgucguugguguuuccau Cel MIMAT0000042 26 hsa-miR-17-5p caaagugcuuacagugcagguag Hu, Ms, Rt MIMAT0000070 27 hsa-miR-181a-5p aacauucaacgcugucggugagu Hu, Ms, Rt MIMAT0000256 28 hsa-miR-181b-5p aacauucauugcugucggugggu Hu, Ms, Rt MIMAT0000257 29 hsa-miR-185-5p uggagagaaaggcaguuccuga Hu, Ms, Rt MIMAT0000455 30 hsa-miR-192-5p cugaccuaugaauugacagcc Hu, Ms, Rt MIMAT0000222 31 hsa-miR-196a-5p uagguaguuucauguuguuggg Hu, Ms, Rt MIMAT0000226 32 hsa-miR-200a-3p uaacacugucugguaacgaugu Hu, Ms, Rt MIMAT0000682 33 hsa-miR-203a-3p gugaaauguuuaggaccacuag Hu, Ms, Rt MIMAT0000264 34 hsa-miR-205-5p uccuucauuccaccggagucug Hu, Ms MIMAT0000266 35 hsa-miR-20a-5p uaaagugcuuauagugcagguag Hu, Ms, Rt MIMAT0000075 36 hsa-miR-20b-5p caaagugcucauagugcagguag Hu, Ms, Rt MIMAT0001413 37 hsa-miR-21-5p uagcuuaucagacugauguuga Hu, Ms, Rt MIMAT0000076 38 hsa-miR-210-3p cugugcgugugacagcggcuga Hu, Ms, Rt MIMAT0000267 39 hsa-miR-22-3p aagcugccaguugaagaacugu Hu, Ms, Rt MIMAT0000077 40 hsa-miR-221-3p agcuacauugucugcuggguuuc Hu, Ms, Rt MIMAT0000278 41 hsa-miR-223-3p ugucaguuugucaaauacccca Hu, Ms MIMAT0000280 42 hsa-miR-23a-3p aucacauugccagggauuucc Hu, Ms, Rt MIMAT0000078 43 hsa-miR-24-3p uggcucaguucagcaggaacag Hu, Ms, Rt MIMAT0000080 44 hsa-miR-26a-5p uucaaguaauccaggauaggcu Hu, Ms, Rt MIMAT0000082 45 hsa-miR-29a-3p uagcaccaucugaaaucgguua Hu, Ms, Rt MIMAT0000086 46 hsa-miR-29b-3p uagcaccauuugaaaucaguguu Hu, Ms, Rt MIMAT0000100 47 hsa-miR-29c-3p uagcaccauuugaaaucgguua Hu, Ms, Rt MIMAT0000681 48 hsa-miR-30a-5p uguaaacauccucgacuggaag Hu, Ms, Rt MIMAT0000087 49 hsa-miR-30b-5p uguaaacauccuacacucagcu Hu, Ms, Rt MIMAT0000420 50 hsa-miR-320a aaaagcuggguugagagggcga Hu, Ms, Rt MIMAT0000510 51 hsa-miR-320d aaaagcuggguugagagga Hu MIMAT0006764 52 hsa-miR-339-5p ucccuguccuccaggagcucacg Hu, Ms, Rt MIMAT0000764 53 hsa-miR-33a-5p gugcauuguaguugcauugca Hu, Ms, Rt MIMAT0000091 54 hsa-miR-342-3p ucucacacagaaaucgcacccgu Hu, Ms, Rt MIMAT0000753 55 hsa-miR-34a-5p uggcagugucuuagcugguugu Hu, Ms, Rt MIMAT0000255 56 hsa-miR-375 uuuguucguucggcucgcguga Hu, Ms, Rt MIMAT0000728 57 oan-miR-7417-5p uuccccacucugagcacacagc Oan MIMAT0028982 58 hsa-miR-422a acuggacuuagggucagaaggc Hu MIMAT0001339 59 hsa-miR-429 uaauacugucugguaaaaccgu Hu MIMAT0001536 60 hsa-miR-431-3p caggucgucuugcagggcuucu Hu, Ms MIMAT0004757 61 hsa-miR-451a aaaccguuaccauuacugaguu Hu, Ms, Rt MIMAT0001631 62 hsa-miR-486-5p uccuguacugagcugccccgag Hu, Ms, Rt MIMAT0002177 63 hsa-miR-494-3p ugaaacauacacgggaaaccuc Hu, Ms MIMAT0002816 64 hsa-miR-523-5p cucuagagggaagcgcuuucug Hu MIMAT0005449 65 hsa-miR-744-5p ugcggggcuagggcuaacagca Hu, Ms MIMAT0004945 66 hsa-miR-885-5p uccauuacacuacccugccucu Hu MIMAT0004947 67 hsa-miR-92a-3p uauugcacuugucccggccugu Hu MIMAT0000092 68 hsa-miR-93-5p caaagugcuguucgugcagguag Hu, Ms, Rt MIMAT0000093 69 x-control x-control N/A 70 blank blank N/A
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 48 tests 1 x 96 tests FirePlex® Particle Mix (Circulating) 1 x 1750µl 1 x 3500µl
ab218369 has been referenced in 2 publications.
- Davies BA et al. Polarized human cholangiocytes release distinct populations of apical and basolateral small extracellular vesicles. Mol Biol Cell 31:2463-2474 (2020). PubMed: 32845745
- Hoss AG et al. miR-10b-5p expression in Huntington's disease brain relates to age of onset and the extent of striatal involvement. BMC Med Genomics 8:10 (2015). PubMed: 25889241