Overview

  • Product name
    FirePlex miRNA Panel – Oncology V2
  • Detection method
    Flow cytometry-fluorescent
  • Assay type
    Multiplex
  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Product overview

    Multiplex miRNA Assay - Oncology panel (ab218367) are the FirePlex® particles designed to profile up to 65 literature curated miRNA for oncology studies, miRNAs (see miRNA list) differentially regulated in one or more solid cancers.


    NOTE: FirePlex miRNA panels require the purchase of a Run Buffer. Use our FirePlex Cytometer Setup Kit (ab211043) to select the most appropriate Run Buffer for your flow cytometer.


    Our multiplex miRNA assays use our FirePlex® particle technology with the same sensitivity as PCR, they can be used either directly with 10 µL of biofluids such as serum or plasma with no need for RNA purification; or with 100 pg of purified RNA.


    For a full list of validated sample types, and to learn more about the performance and requirements of these assays, visit https://www.abcam.com/nav/multiplex-mirna-assays.


    These miRNAs have been chosen based on a detailed study of data in peer-reviewed journals and exhibit differential regulation in whole blood, plasma, serum, peripheral blood mononuclear cells (PBMCs) or isolated exosomes.


    Included in the panel are general markers for multiple cancers, markers specific to specific cancer types, markers that may indicate hemolysis contamination in isolated plasma/serum/exosomes and internal controls to validate assay performance


    It is important to use the normalization procedure in the FirePlex® Analysis Workbench Software to identify the miRNAs that can optimally be used for normalization with your samples. Three miRNAs that are likely to be stably expressed in biofluid samples and may be suitable have been included in this panel.


     

  • Notes

    To use this product researchers must also purchase the appropriate Core Reagent Kit, either ab218365 for use with purified RNA or ab218342 for use with biofluids.

    FirePlex® is a registered trade mark in the United States and is an unregistered trade mark elsewhere. 

    ** Hu = Human, Ms = Mouse, Rt = Rat, Ath = Arabidopsis thaliana, Oan = Ornithorhynchus anatinus, Cel = Caenorhabditis elegans.  

    # miRNA 21 name  miRNA Sequence (miRBase V21) Sequence homology ** MIMAT ID
    1 hsa-let-7d-5p agagguaguagguugcauaguu Hu, Ms, Rt MIMAT0000065
    2 hsa-let-7g-5p ugagguaguaguuuguacaguu Hu, Ms, Rt MIMAT0000414
    3 hsa-let-7i-5p ugagguaguaguuugugcuguu Hu, Ms, Rt MIMAT0000415
    4 hsa-miR-103a-3p agcagcauuguacagggcuauga Hu, Ms, Rt MIMAT0000101
    5 hsa-miR-106a-5p aaaagugcuuacagugcagguag Hu MIMAT0000103
    6 hsa-miR-106b-5p uaaagugcugacagugcagau Hu, Ms, Rt MIMAT0000680
    7 hsa-miR-107 agcagcauuguacagggcuauca Hu, Ms, Rt MIMAT0000104
    8 hsa-miR-10b-5p uacccuguagaaccgaauuugug Hu, Ms, Rt MIMAT0000254
    9 hsa-miR-122-5p uggagugugacaaugguguuug Hu, Ms, Rt MIMAT0000421
    10 hsa-miR-125b-5p ucccugagacccuaacuuguga Hu, Ms, Rt MIMAT0000423
    11 hsa-miR-126-3p ucguaccgugaguaauaaugcg Hu, Ms, Rt MIMAT0000445
    12 hsa-miR-127-3p ucggauccgucugagcuuggcu Hu, Ms, Rt MIMAT0000446
    13 hsa-miR-130a-3p cagugcaauguuaaaagggcau Hu, Ms, Rt MIMAT0000425
    14 hsa-miR-141-3p uaacacugucugguaaagaugg Hu, Ms, Rt MIMAT0000432
    15 hsa-miR-145-5p guccaguuuucccaggaaucccu Hu, Ms, Rt MIMAT0000437
    16 hsa-miR-146a-5p ugagaacugaauuccauggguu Hu, Ms, Rt MIMAT0000449
    17 hsa-miR-148a-3p ucagugcacuacagaacuuugu Hu, Ms MIMAT0000243
    18 hsa-miR-148b-3p ucagugcaucacagaacuuugu Hu, Ms, Rt MIMAT0000759
    19 hsa-miR-150-5p ucucccaacccuuguaccagug Hu, Ms, Rt MIMAT0000451
    20 hsa-miR-151a-3p cuagacugaagcuccuugagg Hu MIMAT0000757
    21 hsa-miR-155-5p uuaaugcuaaucgugauaggggu Hu MIMAT0000646
    22 hsa-miR-15b-5p uagcagcacaucaugguuuaca Hu, Ms, Rt MIMAT0000417
    23 hsa-miR-16-5p uagcagcacguaaauauuggcg Hu, Ms, Rt MIMAT0000069
    24 hsa-miR-17-5p caaagugcuuacagugcagguag Hu, Ms, Rt MIMAT0000070
    25 hsa-miR-181a-5p aacauucaacgcugucggugagu Hu, Ms, Rt MIMAT0000256
    26 hsa-miR-181b-5p aacauucauugcugucggugggu Hu, Ms, Rt MIMAT0000257
    27 hsa-miR-182-5p uuuggcaaugguagaacucacacu Hu MIMAT0000259
    28 hsa-miR-187-3p ucgugucuuguguugcagccgg Hu, Ms, Rt MIMAT0000262
    29 hsa-miR-18a-5p uaaggugcaucuagugcagauag Hu, Ms, Rt MIMAT0000072
    30 hsa-miR-192-5p cugaccuaugaauugacagcc Hu, Ms, Rt MIMAT0000222
    31 hsa-miR-195-5p uagcagcacagaaauauuggc Hu, Ms, Rt MIMAT0000461
    32 hsa-miR-199a-3p acaguagucugcacauugguua Hu, Ms, Rt MIMAT0000232
    33 hsa-miR-199a-5p cccaguguucagacuaccuguuc Hu, Ms, Rt MIMAT0000231
    34 hsa-miR-19a-3p ugugcaaaucuaugcaaaacuga Hu, Ms, Rt MIMAT0000073
    35 hsa-miR-200b-3p uaauacugccugguaaugauga Hu, Ms MIMAT0000318
    36 hsa-miR-200c-3p uaauacugccggguaaugaugga Hu, Ms MIMAT0000617
    37 hsa-miR-205-5p uccuucauuccaccggagucug Hu, Ms MIMAT0000266
    38 hsa-miR-20a-5p uaaagugcuuauagugcagguag Hu, Ms, Rt MIMAT0000075
    39 hsa-miR-210-3p cugugcgugugacagcggcuga Hu, Ms, Rt MIMAT0000267
    40 hsa-miR-21-5p uagcuuaucagacugauguuga Hu, Ms, Rt MIMAT0000076
    41 ath-mir167d ugaagcugccagcaugaucugg Ath MIMAT0000905
    42 hsa-miR-221-3p agcuacauugucugcuggguuuc Hu, Ms, Rt MIMAT0000278
    43 hsa-miR-222-3p agcuacaucuggcuacugggu Hu, Ms, Rt MIMAT0000279
    44 hsa-miR-223-3p ugucaguuugucaaauacccca Hu, Ms MIMAT0000280
    45 hsa-miR-22-3p aagcugccaguugaagaacugu Hu, Ms, Rt MIMAT0000077
    46 hsa-miR-25-3p cauugcacuugucucggucuga Hu, Ms, Rt MIMAT0000081
    47 hsa-miR-26a-5p uucaaguaauccaggauaggcu Hu, Ms, Rt MIMAT0000082
    48 hsa-miR-27a-3p uucacaguggcuaaguuccgc Hu, Ms, Rt MIMAT0000084
    49 hsa-miR-29a-3p uagcaccaucugaaaucgguua Hu, Ms, Rt MIMAT0000086
    50 hsa-miR-29b-3p uagcaccauuugaaaucaguguu Hu, Ms, Rt MIMAT0000100
    51 hsa-miR-29c-3p uagcaccauuugaaaucgguua Hu, Ms, Rt MIMAT0000681
    52 hsa-miR-320a aaaagcuggguugagagggcga Hu, Ms, Rt MIMAT0000510
    53 hsa-miR-335-5p ucaagagcaauaacgaaaaaugu Hu, Ms, Rt MIMAT0000765
    54 hsa-miR-34a-5p uggcagugucuuagcugguugu Hu, Ms, Rt MIMAT0000255
    55 hsa-miR-34b-3p caaucacuaacuccacugccau Hu MIMAT0004676
    56 hsa-miR-375 uuuguucguucggcucgcguga Hu, Ms, Rt MIMAT0000728
    57 hsa-miR-376c-3p aacauagaggaaauuccacgu Hu MIMAT0000720
    58 hsa-miR-378a-3p acuggacuuggagucagaaggc Hu MIMAT0000732
    59 hsa-miR-409-3p gaauguugcucggugaaccccu Hu, Ms MIMAT0001639
    60 hsa-miR-451a aaaccguuaccauuacugaguu Hu, Ms, Rt MIMAT0001631
    61 hsa-miR-483-5p aagacgggaggaaagaagggag Hu MIMAT0004761
    62 hsa-miR-486-5p uccuguacugagcugccccgag Hu, Ms, Rt MIMAT0002177
    63 hsa-miR-574-3p cacgcucaugcacacacccaca Hu, Ms MIMAT0003239
    64 cel-mir-70-3p uaauacgucguugguguuuccau Cel MIMAT0000042
    65 hsa-miR-652-3p aauggcgccacuaggguugug Hu, Ms, Rt MIMAT0003322
    66 hsa-miR-92a-3p uauugcacuugucccggccugu Hu MIMAT0000092
    67 hsa-miR-93-5p caaagugcuguucgugcagguag Hu, Ms, Rt MIMAT0000093
    68 oan-miR-7417-5p uuccccacucugagcacacagc Oan MIMAT0028982
    69 x-control x-control N/A  
    70 blank blank N/A  

     

  • Platform
    Multiplex

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 48 tests 1 x 96 tests
    FirePlex® Particle Mix (Circulating) 1 x 1750µl 1 x 3500µl

Images

  • Samples of human plasma and sera were run in triplicate on the Circulating Oncology Fixed Panel using the Multiplex Circulating miRNA Assay (biofluids). Results were background subtracted and normalized using geometric average, profiling 68 miRNAs per well for each sample.

Protocols

References

This product has been referenced in:
  • Hoss AG  et al. miR-10b-5p expression in Huntington's disease brain relates to age of onset and the extent of striatal involvement. BMC Med Genomics 8:10 (2015). Read more (PubMed: 25889241) »
  • Le MT  et al. miR-200-containing extracellular vesicles promote breast cancer cell metastasis. J Clin Invest 124:5109-28 (2014). Read more (PubMed: 25401471) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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