FirePlex

FirePlex miRNA Panel - Pancreatic Toxicity (ab219510)

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Overview

  • Product name
    FirePlex miRNA Panel - Pancreatic Toxicity
  • Detection method
    Flow cytometry-fluorescent
  • Assay type
    Multiplex
  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Rat, Dog
  • Product overview

    Multiplex miRNA Assay  - Pancreatic Toxicity Panel (ab219510) are the FirePlex® particles designed to profile 32 literature curated miRNA for pancreatic injury and toxicity, miRNAs (see miRNA list), selected based on peer-reviewed publications modeling drug-induced pancreatic toxicity and injury. Included in this panel are miRNAs with pancreas-specific or pancreas-enriched expression, miRNAs identified as circulating biomarkers of pancreatic toxicity and internal controls to validate assay performance.


    NOTE: FirePlex miRNA panels require the purchase of a Run Buffer. Use our FirePlex Cytometer Setup Kit V2 (ab245835) to select the most appropriate Run Buffer for your flow cytometer.


    Our multiplex miRNA assays use our FirePlex® particle technology with the same sensitivity as PCR, they can be used either directly with 10 µL of biofluids such as serum or plasma with no need for RNA purification; or with 100 pg of purified RNA.


    Find a full list of validated sample types, and learn more about the performance and requirements of these assays, here.


    It is important to use the normalization procedure in the FirePlex® Analysis Workbench Software to identify the miRNAs that can optimally be used for normalization with your samples. Three miRNAs that are likely to be stably expressed in biofluid samples and may be suitable have been included in this panel.

  • Notes

    To use this product researchers must also purchase the appropriate Core Reagent Kit, either ab218365 for use with purified RNA or ab218342 for use with biofluids. 

    FirePlex® is a registered trade mark in the United States and is an unregistered trade mark elsewhere. 

    ** Rno = Rattus norvegicus (rat), Cfa = Canis familiaris (dog), Ath = Arabidopsis thaliana (arabidposis), Oan = Ornithorhynchus anatinus (platypus), Cel = Caenorhabditis elegans (worm). 

    #

    miRNA 21 name

    miRNA Sequence (miRBase V21)

    MIMAT ID

    1

    rno-miR-216a

    UAAUCUCAGCUGGCAACUGUGA

    MIMAT0000886

    2

    cfa-miR-216a

    UAAUCUCAGCUGGCAACUGUG

    MIMAT0009849

    3

    rno-miR-216b-5p

    AAAUCUCUGCAGGCAAAUGUGA

    MIMAT0017846

    4

    rno-miR-217

    UACUGCAUCAGGAACUGACUGG

    MIMAT0000887

    5

    cfa-miR-217

    AUACUGCAUCAGGAACUGAUU

    MIMAT0009850

    6

    rno-miR-375-3p

    UUUGUUCGUUCGGCUCGCGUGA

    MIMAT0005307

    7

    rno-miR-148a-3p

    UCAGUGCACUACAGAACUUUG

    MIMAT0035725

    8

    rno-miR-148b-3p

    UCAGUGCAUCACAGAACUUUGU

    MIMAT0000579

    9

    rno-miR-320-3p

    AAAAGCUGGGUUGAGAGGGCGA

    MIMAT0000903

    10

    rno-miR-152-3p

    UCAGUGCAUGACAGAACUUGG

    MIMAT0000854

    11

    rno-mir-92a-3p

    UAUUGCACUUGUCCCGGCCUG

    MIMAT0000816

    12

    rno-miR-96-5p

    UUUGGCACUAGCACAUUUUUGCU

    MIMAT0000818

    13

    rno-miR-129-5p

    CUUUUUGCGGUCUGGGCUUGC

    MIMAT0000600

    14

    rno-miR-133b

    UUUGGUCCCCUUCAACCAGCUA

    MIMAT0003126

    15

    rno-miR-139-5p

    UCUACAGUGCACGUGUCUCCAG

    MIMAT0000845

    16

    rno-mir-188-5p

    CAUCCCUUGCAUGGUGGAGGG

    MIMAT0005301

    17

    rno-miR-204

    UUCCCUUUGUCAUCCUAUGCCU

    MIMAT0000877

    18

    rno-miR-299a-5p

    UGGUUUACCGUCCCACAUACAU

    MIMAT0000901

    19

    rno-miR-337-3p

    UUCAGCUCCUAUAUGAUGCCUUU

    MIMAT0000577

    20

    cfa-mir-371

    ACUCAAAAAAUGGCGGCACUUU

    MIMAT0007747

    21

    rno-miR-383

    CAGAUCAGAAGGUGACUGUGG

    MIMAT0003114

    22

    rno-mir-101a-3p

    UACAGUACUGUGAUAACUGAA

    MIMAT0000823

    23

    rno-miR-141-3p

    UAACACUGUCUGGUAAAGAUGG

    MIMAT0000846

    24

    cfa-miR-141

    AACACUGUCUGGUAAAGAUGG

    MIMAT0009876

    25

    rno-miR-193b-3p

    AACUGGCCCACAAAGUCCC

    MIMAT0035734

    26

    rno-miR-200c-3p

    UAAUACUGCCGGGUAAUGAUG

    MIMAT0000873

    27

    rno-miR-217-5p

    UACUGCAUCAGGAACUGACUGG

    MIMAT0000887

    28

    rno-miR-16-5p

    UAGCAGCACGUAAAUAUUGGCG

    MIMAT0000785

    29

    rno-miR-192-5p

    CUGACCUAUGAAUUGACAGCC

    MIMAT0000867

    30

    rno-miR-29a

    UAGCACCAUCUGAAAUCGGUUA

    MIMAT0000802

    31

    rno-miR-186

    CAAAGAAUUCUCCUUUUGGGCU

    MIMAT0000863

    32

    rno-miR-122

    UGGAGUGUGACAAUGGUGUUUG

    MIMAT0000827

    33

    ath-mir167d

    UGAAGCUGCCAGCAUGAUCUGG

    MIMAT0000905

    34

    cel-mir-70-3p

    UAAUACGUCGUUGGUGUUUCCAU

    MIMAT0000042

    35

    oan-miR-7417-5p

    UUCCCCACUCUGAGCACACAGC

    MIMAT0028982

    36

    x-control

    x-control

     

    37

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  • Platform
    Multiplex

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 48 tests 1 x 96 tests
    FirePlex® Particle Mix (Circulating) 1 x 1750µl 1 x 3500µl

Protocols

References

ab219510 has not yet been referenced specifically in any publications.

Publishing research using ab219510? Please let us know so that we can cite the reference in this datasheet.

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