FirePlex Mouse Key Cytokines - Immunoassay Panel (ab235656)
Overview
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Product name
FirePlex Mouse Key Cytokines - Immunoassay Panel -
Detection method
Flow cytometry-fluorescent -
Sample type
Cell culture supernatant, Urine, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasma, Tissue Homogenate -
Assay type
Multiplex -
Assay time
3h 30m -
Species reactivity
Reacts with: Mouse -
Product overview
FirePlex Mouse Key Cytokines - Immunoassay Panel (ab235656) contains all the reagents you need to run a 17-plex assay: antibody conjugated particles, Rodent Core Reagents and Protein Standard Mix.
NOTE: FirePlex Immunoassay panels require the purchase of a Run Buffer. Use our FirePlex Cytometer Setup Kit V2 (ab245835) to select the most appropriate Run Buffer for your flow cytometer.
Assay run-time is 3.5 hours, followed by particle analysis using a validated flow cytometer model and data analysis using our integrated, free-of-charge FirePlex Analysis Workbench software.
FirePlex® is a registered trade mark in the United States and is registered as a European Union Trade Mark.
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Notes
This panel was previously available under the product code ab213397, which is discontinued. We have redeveloped this panel using new, recombinant monoclonal antibodies that provide higher sensitivity and better reproducibility when quantifying the analytes with biofluid samples, improving consistency in kit performance.
The following information provides the sensitivity, range, and required Protein Standard Mix.
Target
Sensitivity (pg/mL)
Dynamic Range (pg/mL)
Protein Standard
CXCL1
0.07
0.51 - 1,111
Mix A
GM-CSF
0.29
1.52 - 3,333
Mix A
IFN gamma
0.09
0.51 - 1,111
Mix A
IL-1 beta
0.23
1.52 - 3,333
Mix A
IL-2
0.83
4.57 - 10,000
Mix A
IL-4
0.24
1.52 - 3,333
Mix A
IL-5
0.13
0.51 - 1,111
Mix A
IL-6
8.47
13.7 - 30,000
Mix A
IL-9
0.09
0.51 - 1,111
Mix A
IL-10
1.59
4.57 - 10,000
Mix A
IL-12p70
1.28
4.57 - 10,000
Mix A
IL-13
0.37
1.52 - 3,333
Mix A
IL-17A
0.17
0.51 - 1,111
Mix A
MCP1
0.09
0.51 - 1,111
Mix A
MIP1a
0.49
1.52 - 3,333
Mix A
MIP1b
0.07
0.51 - 1,111
Mix A
TNF alpha
0.37
1.52 - 3,333
Mix A
Negative Control Particles
N/A
N/A
N/A
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Platform
Multiplex
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Key Cytokines Capture Particles (ab235656) 1 x 1800µl 10X Wash Buffer 1 x 25ml 15X Mouse Key Cytokines Biotin Detectors (ab235656) 1 x 400µl 2X Rodent Assay Diluent 1 x 15ml 5X Reporter Solution 1 x 4ml 96-well microplate with a filter 1 unit Filter Plate Seal 3 units Mouse Multiplex Immunoassay Protein Standard Mix A 1 x 200µl Tungsten cleaning wire 1 unit -
Research areas
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Cellular localization
TNF alpha: Secreted and Cell membrane. MCP1: Secreted. IL6: Secreted. GM-CSF: Secreted. IL10: Secreted. Interferon gamma: Secreted. IL13: Secreted. IL9: Secreted. IL5: Secreted. GRO alpha: Secreted. IL1 beta: Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive. Macrophage Inflammatory Protein 1 alpha / CCL3: Secreted. Macrophage Inflammatory Protein 1 beta: Secreted. IL2: Secreted. IL4: Secreted. IL12 p70: Secreted. IL17A: Secreted. -
Database links
- Entrez Gene: 12981 Mouse
- Entrez Gene: 14825 Mouse
- Entrez Gene: 15978 Mouse
- Entrez Gene: 16153 Mouse
- Entrez Gene: 16159 Mouse
- Entrez Gene: 16163 Mouse
- Entrez Gene: 16171 Mouse
- Entrez Gene: 16176 Mouse
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Associated products
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Related Buffer
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Related Products
Images
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Example of FirePlex Mouse Key Cytokines - Immunoassay Panel standard curves.
Mean background-subtracted data values are graphed (+/- SD).
References (0)
ab235656 has not yet been referenced specifically in any publications.
