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  1. Link

    fireplex-mouse-key-cytokines-immunoassay-panel-ab235656.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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FirePlex

FirePlex Mouse Key Cytokines - Immunoassay Panel (ab235656)

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  • Protocol Booklet
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Example of FirePlex Mouse Key Cytokines - Immunoassay Panel standard curves.

    Overview

    • Product name

      FirePlex Mouse Key Cytokines - Immunoassay Panel
    • Detection method

      Flow cytometry-fluorescent
    • Sample type

      Cell culture supernatant, Urine, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasma, Tissue Homogenate
    • Assay type

      Multiplex
    • Assay time

      3h 30m
    • Species reactivity

      Reacts with: Mouse
    • Product overview

      FirePlex Mouse Key Cytokines - Immunoassay Panel (ab235656) contains all the reagents you need to run a 17-plex assay: antibody conjugated particles, Rodent Core Reagents and Protein Standard Mix.


      NOTE: FirePlex Immunoassay panels require the purchase of a Run Buffer. Use our FirePlex Cytometer Setup Kit V2 (ab245835) to select the most appropriate Run Buffer for your flow cytometer.


      Assay run-time is 3.5 hours, followed by particle analysis using a validated flow cytometer model and data analysis using our integrated, free-of-charge FirePlex Analysis Workbench software.


      FirePlex® is a registered trade mark in the United States and is registered as a European Union Trade Mark.

    • Notes

      This panel was previously available under the product code ab213397, which is discontinued. We have redeveloped this panel using new, recombinant monoclonal antibodies that provide higher sensitivity and better reproducibility when quantifying the analytes with biofluid samples, improving consistency in kit performance.

      The following information provides the sensitivity, range, and required Protein Standard Mix.

      Target

      Sensitivity (pg/mL)

      Dynamic Range (pg/mL)

      Protein Standard

      CXCL1

      0.07

      0.51 - 1,111

      Mix A

      GM-CSF

      0.29

      1.52 - 3,333

      Mix A

      IFN gamma

      0.09

      0.51 - 1,111

      Mix A

      IL-1 beta

      0.23

      1.52 - 3,333

      Mix A

      IL-2

      0.83

      4.57 - 10,000

      Mix A

      IL-4

      0.24

      1.52 - 3,333

      Mix A

      IL-5

      0.13

      0.51 - 1,111

      Mix A

      IL-6

      8.47

      13.7 - 30,000

      Mix A

      IL-9

      0.09

      0.51 - 1,111

      Mix A

      IL-10

      1.59

      4.57 - 10,000

      Mix A

      IL-12p70

      1.28

      4.57 - 10,000

      Mix A

      IL-13

      0.37

      1.52 - 3,333

      Mix A

      IL-17A

      0.17

      0.51 - 1,111

      Mix A

      MCP1

      0.09

      0.51 - 1,111

      Mix A

      MIP1a

      0.49

      1.52 - 3,333

      Mix A

      MIP1b

      0.07

      0.51 - 1,111

      Mix A

      TNF alpha

      0.37

      1.52 - 3,333

      Mix A

      Negative Control Particles

      N/A

      N/A

      N/A

       
    • Platform

      Multiplex

    Properties

    • Storage instructions

      Please refer to protocols.
    • Components 1 x 96 tests
      10X Mouse Key Cytokines Capture Particles (ab235656) 1 x 1800µl
      10X Wash Buffer 1 x 25ml
      15X Mouse Key Cytokines Biotin Detectors (ab235656) 1 x 400µl
      2X Rodent Assay Diluent 1 x 15ml
      5X Reporter Solution 1 x 4ml
      96-well microplate with a filter 1 unit
      Filter Plate Seal 3 units
      Mouse Multiplex Immunoassay Protein Standard Mix A 1 x 200µl
      Tungsten cleaning wire 1 unit
    • Research areas

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Immunology
      • Adaptive Immunity
      • B Cells
      • Non-CD
      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
      • Immunology
      • Innate Immunity
      • Cytokines
      • Interferons
      • Immunology
      • Innate Immunity
      • Cytokines
      • CSFs
      • Immunology
      • Innate Immunity
      • Cytokines
      • TNF Superfamily
      • Immunology
      • Innate Immunity
      • Chemokines
      • Alpha Chemokines (CXC)
      • Immunology
      • Innate Immunity
      • Chemokines
      • Beta Chemokines (CC)
      • Signal Transduction
      • Growth Factors/Hormones
      • TNF
      • Neuroscience
      • Neurology process
      • Metabolism
      • Stem Cells
      • Signaling Pathways
      • TGF beta
      • Interferon gamma
      • Cancer
      • Growth factors
      • TNF
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • Angiogenic growth factors
      • Cancer
      • Tumor immunology
      • Cytokines
      • Interferons
      • Cancer
      • Tumor immunology
      • Cytokines
      • Interleukins
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Inflammatory mediators
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Leukocyte recruitment
      • Chemokines
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      • Regulatory T Cells
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      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
      • Metabolism
      • Types of disease
      • Obesity
      • Metabolism
      • Types of disease
      • Heart disease
      • Metabolism
      • Types of disease
      • Metabolic disorders
      • Cardiovascular
      • Angiogenesis
      • Endothelial Cell Markers
    • Cellular localization

      TNF alpha: Secreted and Cell membrane. MCP1: Secreted. IL6: Secreted. GM-CSF: Secreted. IL10: Secreted. Interferon gamma: Secreted. IL13: Secreted. IL9: Secreted. IL5: Secreted. GRO alpha: Secreted. IL1 beta: Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive. Macrophage Inflammatory Protein 1 alpha / CCL3: Secreted. Macrophage Inflammatory Protein 1 beta: Secreted. IL2: Secreted. IL4: Secreted. IL12 p70: Secreted. IL17A: Secreted.
    • Database links

      • Entrez Gene: 12981 Mouse
      • Entrez Gene: 14825 Mouse
      • Entrez Gene: 15978 Mouse
      • Entrez Gene: 16153 Mouse
      • Entrez Gene: 16159 Mouse
      • Entrez Gene: 16163 Mouse
      • Entrez Gene: 16171 Mouse
      • Entrez Gene: 16176 Mouse
      • Entrez Gene: 16183 Mouse
      • Entrez Gene: 16189 Mouse
      • Entrez Gene: 16191 Mouse
      • Entrez Gene: 16193 Mouse
      • Entrez Gene: 16198 Mouse
      • Entrez Gene: 20296 Mouse
      • Entrez Gene: 20302 Mouse
      • Entrez Gene: 20303 Mouse
      • Entrez Gene: 21926 Mouse
      • SwissProt: P01580 Mouse
      • SwissProt: P01587 Mouse
      • SwissProt: P04351 Mouse
      • SwissProt: P04401 Mouse
      • SwissProt: P06804 Mouse
      • SwissProt: P07750 Mouse
      • SwissProt: P08505 Mouse
      • SwissProt: P10148 Mouse
      • SwissProt: P10749 Mouse
      • SwissProt: P10855 Mouse
      • SwissProt: P12850 Mouse
      • SwissProt: P14097 Mouse
      • SwissProt: P15247 Mouse
      • SwissProt: P18893 Mouse
      • SwissProt: P20109 Mouse
      • SwissProt: P43431 Mouse
      • SwissProt: Q62386 Mouse
      • Unigene: 1019 Mouse
      • Unigene: 103783 Mouse
      • Unigene: 1282 Mouse
      • Unigene: 1284 Mouse
      • Unigene: 1293 Mouse
      • Unigene: 14190 Mouse
      • Unigene: 21013 Mouse
      • Unigene: 222830 Mouse
      • Unigene: 240327 Mouse
      • Unigene: 244263 Mouse
      • Unigene: 276360 Mouse
      • Unigene: 290320 Mouse
      • Unigene: 3006 Mouse
      • Unigene: 4461 Mouse
      • Unigene: 4922 Mouse
      • Unigene: 874 Mouse
      see all

    Associated products

    • Related Buffer

      • FirePlex Cytometer Run Buffer II (ab234450)
      • FirePlex Cytometer Run Buffer I (ab245836)
      • FirePlex Cytometer Run Buffer III (ab245837)
    • Related Products

      • FirePlex Cytometer Setup Kit V2 (ab245835)

    Images

    • Example of FirePlex Mouse Key Cytokines - Immunoassay Panel standard curves.
      Example of FirePlex Mouse Key Cytokines - Immunoassay Panel standard curves.

      Mean background-subtracted data values are graphed (+/- SD).

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab235656? Please let us know so that we can cite the reference in this datasheet.

    ab235656 has not yet been referenced specifically in any publications.

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