Overview

  • Product name

    FirePlex Mouse Kidney Toxicity - Immunoassay Panel
  • Detection method

    Flow cytometry-fluorescent
  • Sample type

    Cell culture supernatant, Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Multiplex
  • Assay time

    3h 30m
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    FirePlex Mouse Kidney Toxicity - Immunoassay Panel (ab235661) contains all the reagents you need to run a 5-plex assay: antibody conjugated particles, Rodent Core Reagents and Protein Standard Mix.


    NOTE: FirePlex Immunoassay panels require the purchase of a Run Buffer. Use our FirePlex Cytometer Setup Kit V2 (ab245835) to select the most appropriate Run Buffer for your flow cytometer.


    Assay run-time is 3.5 hours, followed by particle analysis using a validated flow cytometer model and data analysis using our integrated, free-of-charge FirePlex Analysis Workbench software.


    FirePlex® is a registered trade mark in the United States and is registered as a European Union Trade Mark.

  • Notes

    The following information provides the sensitivity, range, CVs, and required Protein Standard Mix. 

    Target

    Sensitivity (pg/mL)

    Dynamic Range (pg/mL)

    Protein Standard

    Clusterin 

     23.00

    123.45 - 270,000 

    Mouse Kidney Toxicity Protein Standard Mix

     Cystatin C

    0.15 

    1.52 - 3,333 

    Mouse Kidney Toxicity Protein Standard Mix

     KIM-1

     0.07

    0.51 - 1,111 

    Mouse Kidney Toxicity Protein Standard Mix

     Osteopontin

     12.29

     41.15 - 90,000

    Mouse Kidney Toxicity Protein Standard Mix

     Lipocalin-2

     0.06

    0.51-1,111 

    Mouse Kidney Toxicity Protein Standard Mix

    Negative Control Particles

    N/A

    N/A

    N/A

  • Platform

    Multiplex

Properties

Images

  • Mean background-subtracted data values are graphed (+/- SD).

Protocols

References

ab235661 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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