Overview

  • Product name

    FirePlex Mouse Th1/Th2 - Immunoassay Panel
  • Detection method

    Flow cytometry-fluorescent
  • Sample type

    Cell culture supernatant, Saliva, Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Multiplex
  • Assay time

    3h 30m
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    FirePlex Mouse Th1/Th2 - Immunoassay Panel (ab235657) contains all the reagents you need to run a 7-plex assay: antibody conjugated particles, Rodent Core Reagents and Protein Standard Mix.


    NOTE: FirePlex Immunoassay panels require the purchase of a Run Buffer. Use our FirePlex Cytometer Setup Kit V2 (ab245835) to select the most appropriate Run Buffer for your flow cytometer.


    Assay run-time is 3.5 hours, followed by particle analysis using a validated flow cytometer model and data analysis using our integrated, free-of-charge FirePlex Analysis Workbench software.


     FirePlex® is a registered trade mark in the United States and is registered as a European Union Trade Mark.

  • Notes

     

    This panel was previously available under the product code ab213394, which is discontinued. We have redeveloped this panel using new, recombinant monoclonal antibodies that provide higher sensitivity and better reproducibility when quantifying the analytes with biofluid samples, improving consistency in kit performance.

     

    The following information provides the sensitivity, range, CVs, and required Protein Standard Mix.

    Target

    Sensitivity (pg/mL)

    Dynamic Range (pg/mL)

    Protein Standard

    IFN-gamma

    0.09

    0.51 - 1,111

    Mix A

    IL-10

    1.59

    4.57 - 10,000

    Mix A

    IL-2

    0.83

    4.57 - 10,000

    Mix A

    IL-4

    0.24

    1.52 - 3,333

    Mix A

    IL-5

    0.13

    0.51 - 1,111

    Mix A

    IL-6

    8.47

    13.72 - 30,000

    Mix A

    TNF-alpha

    0.37

    1.52 - 3,333

    Mix A

    Negative Control Particles

    N/A

    N/A

    N/A

  • Platform

    Multiplex

Properties

Images

  • Mean background-subtracted data values are graphed (+/- SD).

Protocols

References

ab235657 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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