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Cardiovascular Angiogenesis Cytokines Interleukin
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FirePlex

FirePlex NHP Key Cytokines - Immunoassay Panel (ab239455)

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  • Protocol Booklet
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Example of NHP Key Cytokines (16-plex) standard curves

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    Overview

    • Product name

      FirePlex NHP Key Cytokines - Immunoassay Panel
    • Detection method

      Flow cytometry-fluorescent
    • Sample type

      Cell culture supernatant, Urine, Serum, Cell culture extracts, Other biological fluids, Hep Plasma, EDTA Plasma, Cit plasma, Tissue Homogenate
    • Assay type

      Multiplex
    • Assay time

      3h 30m
    • Species reactivity

      Reacts with: Non human primates
    • Product overview

      FirePlex NHP Key Cytokines - Immunoassay Panel (ab239455) contains all the reagents you need to run a 16-plex assay: antibody conjugated particles, non-human primate (NHP) Core Reagents and Protein Standard Mix.


      Assay run-time is 3.5 hours, followed by particle analysis using a validated flow cytometer model and data analysis using our integrated, free-of-charge FirePlex Analysis Workbench software.


      FirePlex® is a registered trade mark in the United States and is registered as a European Union Trade Mark.

    • Notes

      The following information provides the sensitivity, range, and required Protein Standard Mix.

      Target

      Sensitivity (pg/mL)

      Dynamic Range (pg/mL)

      Protein Standard

      GM-CSF

      1.90

      13.7 - 30,000

      NHP Key Cyto

      IFN gamma

      2.67

      13.7 - 30,000

      NHP Key Cyto

      IL-1 beta

      0.25

      1.52 - 3,333

      NHP Key Cyto

      IL-2

      10.65

      41.1 - 90,000

      NHP Key Cyto

      IL-4

      1.19

      4.57 - 10,000

      NHP Key Cyto

      IL-5

      0.24

      1.52 - 3,333

      NHP Key Cyto

      IL-6

      3.02

      13.7 - 30,000

      NHP Key Cyto

      IL-8

      0.14

      0.51 - 1,111

      NHP Key Cyto

      IL-10

      2.34

      13.7 - 30,000

      NHP Key Cyto

      IL-12p70

      8.53

      41.1 - 90,000

      NHP Key Cyto

      IL-13

      5.63

      13.7 - 30,000

      NHP Key Cyto

      IL-17A

      7.10

      13.7 - 30,000

      NHP Key Cyto

      MCP1

      4.71

      13.7 - 30,000

      NHP Key Cyto

      MIP1a

      3.10

      13.7 - 30,000

      NHP Key Cyto

      MIP1b

      0.14

      0.51 - 1,111

      NHP Key Cyto

      TNF alpha

      0.61

      4.57 - 10,000

      NHP Key Cyto

      Negative Control Particle

      N/A

      N/A

      N/A

    • Platform

      Multiplex

    Properties

    • Storage instructions

      Please refer to protocols.
    • Components 1 x 96 tests
      10X NHP Key Cytokines Panel Capture Particles 1 vial
      10X Wash Buffer 1 x 25ml
      15X NHP Key Cytokines Panel Biotin Detectors 1 vial
      2X Human & NHP Assay Diluent 1 x 15ml
      5X Reporter Solution 1 x 4ml
      96-well microplate with a filter 1 unit
      Filter Plate Seal 1 x 3 units
      NHP Key Cytokines Protein Standard Mix 1 vial
      Tungsten cleaning wire 1 unit
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Cytokines
      • Interleukin
      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Immunology
      • Adaptive Immunity
      • B Cells
      • Non-CD
      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
      • Immunology
      • Innate Immunity
      • Cytokines
      • Interferons
      • Immunology
      • Innate Immunity
      • Cytokines
      • CSFs
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      • Tumor immunology
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      • Interleukins
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    • Cellular localization

      TNF alpha: Secreted and Cell membrane. IL-8: Secreted. MCP1: Secreted. IL-6: Secreted. GM-CSF: Secreted. IL-10: Secreted. Interferon gamma: Secreted. IL-13: Secreted. IL-5: Secreted. IL-1 beta: Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive. Macrophage Inflammatory Protein 1 beta: Secreted. IL-2: Secreted. IL-4: Secreted. MIP 1 alpha: Secreted. IL12 p70: Secreted. IL-17A: Secreted.

    Associated products

    • Related Buffer

      • FirePlex Cytometer Run Buffer 2 (ab234450)
      • FirePlex Cytometer Run Buffer 1 (ab245836)
      • FirePlex Cytometer Run Buffer 3 (ab245837)
    • Related Products

      • FirePlex Cytometer Setup Kit V2 (ab245835)

    Images

    • Example of NHP Key Cytokines (16-plex) standard curves
      Example of NHP Key Cytokines (16-plex) standard curves

      Example of NHP Key Cytokines Immunoassay standard curves.  Mean background-subtracted data values are graphed (+/- SD).

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab239455? Please let us know so that we can cite the reference in this datasheet.

    ab239455 has not yet been referenced specifically in any publications.

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