Overview

  • Product name
    FirePlex NHP Key Cytokines - Immunoassay Panel
  • Detection method
    Flow cytometry-fluorescent
  • Sample type
    Cell culture supernatant, Serum, Plasma, Other biological fluids
  • Assay type
    Multiplex
  • Assay time
    3h 30m
  • Species reactivity
    Reacts with: Non human primates
  • Product overview

    FirePlex NHP Key Cytokines - Immunoassay Panel (ab239455) contains all the reagents you need to run a 16-plex assay: antibody conjugated particles, non-human primate (NHP) Core Reagents and Protein Standard Mix.


    Assay run-time is 3.5 hours, followed by particle analysis using a validated flow cytometer model and data analysis using our integrated, free-of-charge FirePlex Analysis Workbench software.


    FirePlex® is a registered trade mark in the United States and is registered as a European Union Trade Mark.

  • Notes

    The following information provides the sensitivity, range, CVs, and required Protein Standard Mix.

    Target

    Sensitivity (pg/mL)

    Dynamic Range (pg/mL)

    Protein Standard

    GM-CSF

    1.90

    13.72 - 30,000

    NHP Key Cyto

    IFN-gamma

    2.67

    13.72 - 30,000

    NHP Key Cyto

    IL-1 beta

    0.25

    1.52 - 3,333

    NHP Key Cyto

    IL-10

    2.34

    13.72 - 30,000

    NHP Key Cyto

    IL-12p70

    8.53

    41.15 - 90,000

    NHP Key Cyto

    IL-13

    5.63

    13.72 - 30,000

    NHP Key Cyto

    IL-17A

    7.10

    13.72 - 30,000

    NHP Key Cyto

    IL-2

    10.65

    41.15 - 90,000

    NHP Key Cyto

    IL-4

    1.19

    4.57 - 10,000

    NHP Key Cyto

    IL-5

    0.24

    1.52 - 3,333

    NHP Key Cyto

    IL-6

    3.02

    13.72 - 30,000

    NHP Key Cyto

    IL-8

    0.14

    0.51 - 1,111

    NHP Key Cyto

    MCP1

    4.71

    13.72 - 30,000

    NHP Key Cyto

    MIP1a

    3.10

    13.72 - 30,000

    NHP Key Cyto

    MIP1b

    0.14

    0.51 - 1,111

    NHP Key Cyto

    TNF-alpha

    0.61

    4.57 - 10,000

    NHP Key Cyto

    Negative Control Particle

    N/A

    N/A

    N/A

  • Platform
    Multiplex

Properties

Images

  • Example of NHP Key Cytokines Immunoassay standard curves.  Mean background-subtracted data values are graphed (+/- SD).

Protocols

References

ab239455 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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