FirePlex NHP Key Cytokines - Immunoassay Panel (ab239455)
- Datasheet
- References
- Protocols
Overview
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Product name
FirePlex NHP Key Cytokines - Immunoassay Panel -
Detection method
Flow cytometry-fluorescent -
Sample type
Cell culture supernatant, Urine, Serum, Cell culture extracts, Other biological fluids, Hep Plasma, EDTA Plasma, Cit plasma, Tissue Homogenate -
Assay type
Multiplex -
Assay time
3h 30m -
Species reactivity
Reacts with: Non human primates -
Product overview
FirePlex NHP Key Cytokines - Immunoassay Panel (ab239455) contains all the reagents you need to run a 16-plex assay: antibody conjugated particles, non-human primate (NHP) Core Reagents and Protein Standard Mix.
Assay run-time is 3.5 hours, followed by particle analysis using a validated flow cytometer model and data analysis using our integrated, free-of-charge FirePlex Analysis Workbench software.
FirePlex® is a registered trade mark in the United States and is registered as a European Union Trade Mark.
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Notes
The following information provides the sensitivity, range, and required Protein Standard Mix.
Target
Sensitivity (pg/mL)
Dynamic Range (pg/mL)
Protein Standard
GM-CSF
1.90
13.72 - 30,000
NHP Key Cyto
IFN-gamma
2.67
13.72 - 30,000
NHP Key Cyto
IL-1 beta
0.25
1.52 - 3,333
NHP Key Cyto
IL-10
2.34
13.72 - 30,000
NHP Key Cyto
IL-12p70
8.53
41.15 - 90,000
NHP Key Cyto
IL-13
5.63
13.72 - 30,000
NHP Key Cyto
IL-17A
7.10
13.72 - 30,000
NHP Key Cyto
IL-2
10.65
41.15 - 90,000
NHP Key Cyto
IL-4
1.19
4.57 - 10,000
NHP Key Cyto
IL-5
0.24
1.52 - 3,333
NHP Key Cyto
IL-6
3.02
13.72 - 30,000
NHP Key Cyto
IL-8
0.14
0.51 - 1,111
NHP Key Cyto
MCP1
4.71
13.72 - 30,000
NHP Key Cyto
MIP1a
3.10
13.72 - 30,000
NHP Key Cyto
MIP1b
0.14
0.51 - 1,111
NHP Key Cyto
TNF-alpha
0.61
4.57 - 10,000
NHP Key Cyto
Negative Control Particle
N/A
N/A
N/A
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Platform
Multiplex
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 10X NHP Key Cytokines Panel Capture Particles 1 vial 10X Wash Buffer 1 x 25ml 15X NHP Key Cytokines Panel Biotin Detectors 1 vial 2X Human & NHP Assay Diluent 1 x 15ml 5X Reporter Solution 1 x 4ml 96-well microplate with a filter 1 unit Filter Plate Seal 1 x 3 units NHP Key Cytokines Protein Standard Mix 1 vial Tungsten cleaning wire 1 unit -
Research areas
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Cellular localization
TNF alpha: Secreted and Cell membrane. IL-8: Secreted. MCP1: Secreted. IL-6: Secreted. GM-CSF: Secreted. IL-10: Secreted. Interferon gamma: Secreted. IL-13: Secreted. IL-5: Secreted. IL-1 beta: Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive. Macrophage Inflammatory Protein 1 beta: Secreted. IL-2: Secreted. IL-4: Secreted. MIP 1 alpha: Secreted. IL12 p70: Secreted. IL-17A: Secreted.
Associated products
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Related Buffer
Images
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Example of NHP Key Cytokines (16-plex) standard curvesExample of NHP Key Cytokines Immunoassay standard curves. Mean background-subtracted data values are graphed (+/- SD).
Protocols
References
ab239455 has not yet been referenced specifically in any publications.
