Overview

  • Product name

    FirePlex Rat Th1/Th2 - Immunoassay Panel
  • Detection method

    Flow cytometry-fluorescent
  • Sample type

    Cell culture supernatant, Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Multiplex
  • Assay time

    3h 30m
  • Species reactivity

    Reacts with: Rat
  • Product overview

    FirePlex Rat Th1/Th2 - Immunoassay Panel (ab235663) contains all the reagents you need to run a 7-plex assay: antibody conjugated particles, Rodent Core Reagents and Protein Standard Mix.


    NOTE: FirePlex Immunoassay panels require the purchase of a Run Buffer. Use our FirePlex Cytometer Setup Kit V2 (ab245835) to select the most appropriate Run Buffer for your flow cytometer.


    Assay run-time is 3.5 hours, followed by particle analysis using a validated flow cytometer model and data analysis using our integrated, free-of-charge FirePlex Analysis Workbench software.


    FirePlex® is a registered trade mark in the United States and is registered as a European Union Trade Mark.

  • Notes

    The following information provides the sensitivity, range, CVs, and required Protein Standard Mix.

    Target

    Sensitivity (pg/mL)

    Dynamic Range (pg/mL)

    Protein Standard

    IFN-gamma

    1.07

    1.52 – 3,333

    Mix A

    IL-10

    0.74

    4.57 – 10,000

    Mix A

    IL-2

    0.37

    1.52 – 3,333

    Mix A

    IL-4

    0.84

    1.52 – 3,333

    Mix A

    IL-5

    1.38

    4.57 – 10,000

    Mix A

    IL-6

    7.50

    13.72 – 30,000

    Mix A

    TNF-alpha

    3.36

    4.57 – 10,000

    Mix A

    Negative Control Particles

    N/A

    N/A

    N/A

     
  • Platform

    Multiplex

Properties

Images

  • Mean background-subtracted data values are graphed (+/- SD).

Protocols

References

ab235663 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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