Product nameFirePlex®-384 Cardiotoxicity (Human) Immunoassay Panel
Sample typeCell culture supernatant, Serum, Plasma, Hep Plasma, EDTA Plasma, Cit plasma
Species reactivityReacts with: Human
FirePlex®-384 Cardiotoxicity (Human) Immunoassay Panel (ab252375) uses FirePlex® particle technology to quantify ICAM1, Cardiac Troponin I, FABP3, NT-proBNP, and BNP from 6.25 µl of sample input in 384-well plate format.
Assays are validated in serum, plasma, and cell culture supernatant, and can be run with 175 samples in duplicate per 384-well plate.
The two-step workflow and no-wash assay format limit hands-on time and are amenable to automation, thus making FirePlex®-384 ideally suited for high-throughput screening studies. Assay readout is conducted on high-content imagers with data analysis using the FirePlex® Analysis Workbench software.
Users should note that assay performance data supplied in the accompanying Product Datasheet is a representative dataset, and is not lot-specific. For lot-specific assay performance data, please refer to the product's certificate of analysis, which can be found here.
FirePlex® is a registered trade mark in certain territories (including Europe and the United States) and is an unregistered trade mark elsewhere.
This product is currently only supported for customers in North America and Europe, if you are outside these areas please contact us.
The following table provides the sensitivity, dynamic range, and CVs for FirePlex®-384 Cardiotoxicity (Human) Immunoassay Panel *.
BNP 8.71 13.7 - 3,333 13.3% Cardiac Troponin I∇ 92.60 123 - 30,000 9.2% FABP3∇ 6.42 13.7 - 1,111 8.6% ICAM1 26.79 41.1 - 30,000 10.7% NT-proBNP 15.53 41.1 - 3,333 11.7%
*representative dataset, not lot-specific. For lot-specific assay performance data, please refer to the product's certificate of analysis, which can be found here.
∇The bovine and human proteins for these analytes exhibit a >90% sequence homology, thus some bovine serum cross-reactivity may be observed in cell culture supernatant (CCS) samples. To address this, we strongly recommend that users include a cell culture media-only control in experiments using CCS samples.
Storage instructionsPlease refer to protocols.
Components 1 x 384 tests 384 Well Imaging Plate 1 x 384 tests 384 Well Imaging Plate Lid 1 unit 384 Well Imaging Plate Seal 2 units FirePlex-384 1X PBS 1 x 8ml FirePlex-384 5X Capture Particle Mix 1 x 700µl FirePlex-384 Human Protein Standard Mix HTCTX 1 x 7 tests FirePlex-384 Reagent Diluent R1 1 x 8ml FirePlex-384 Sample Diluent S1 1 x 25ml FirePlex-384 - Imaging Dye Concentrate 1 x 875µl FirePlex-384 35X Detector Antibody Mix 1 x 110µl
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Cellular localizationICAM1: Membrane. H-FABP: Cytoplasm. proBNP: Secreted. BNP: Secreted
ab252375 has not yet been referenced specifically in any publications.