Overview

  • Product name
    FirePlex® miRNA Assay Core Reagent Kit V2 - Biofluid
  • Product overview

    The FirePlex® miRNA Assay enables high-throughput detection by flow cytometry of up to 65 microRNA targets and streamlined analysis with included FirePlex® Analysis Workbench software.


    Our multiplex miRNA assays use our FirePlex® particle technology to profile up to 65 miRNA directly from 10 µL of biofluid, without the need for RNA purification. The assay provides PCR sensitivity while eliminating the need for separate reverse transcription reactions and mitigating amplification biases introduced by target-specific qPCR. This is made possible by combining uniquely encoded FirePlex® hydrogel particles with single-step RT-PCR signal amplification using universal primers. The entire assay is performed in 96-well plate format such that users detect up to 65 microRNA targets for each of 96 samples in less than a day’s work.


    The FirePlex® miRNA Assay reliably detects as few as 1000 microRNA copies per sample with a linear dynamic range of ~5 logs. In addition to increased sensitivity, the assay's multiplexed detection conserves precious sample by detecting multiple microRNA targets from only 10 µL of biofluid, and has been validated for numerous sample types including plasma, serum, exosomes, urine and saliva.


    For a full list of validated sample types, and to learn more about the performance and requirements of these assays, visit https://www.abcam.com/nav/multiplex-mirna-assays.

  • Notes

    This product is currently only supported for customers in North America and Europe, if you are outside these areas please contact us.

    FirePlex® is a registered trade mark in the United States and is an unregistered trade mark elsewhere.

Properties

  • Storage instructions
    Please refer to protocols.
  • Components 1 x 96 tests
    10x Rinse Buffer A 1 x 30ml
    1x Hyb Buffer  1 x 25ml
    5x Reporter Solution 1 x 4ml
    Filter Plate 2 units
    Catch Plate 2 units
    RNA Control 1 x 50µl
    Digest Buffer 1 x 4ml
    dNTP mix 1 x 120µl
    50x Labeling Buffer 1 x 200µl
    Labeling Diluent 1 x 30ml
    Labeling Enzyme 1 x 50µl
    PCR Buffer 1 x 2ml
    PCR Enzyme 1 x 60µl
    Primer Mix 1 x 250µl
    Protease Mix 1 x 500µl
    2x Rinse Buffer B 1 x 33ml
    Run buffer 1 x 30ml

Protocols

References

This product has been referenced in:
  • Hoss AG  et al. miR-10b-5p expression in Huntington's disease brain relates to age of onset and the extent of striatal involvement. BMC Med Genomics 8:10 (2015). Read more (PubMed: 25889241) »
  • Le MT  et al. miR-200-containing extracellular vesicles promote breast cancer cell metastasis. J Clin Invest 124:5109-28 (2014). Read more (PubMed: 25401471) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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