For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
The FirePlex® miRNA Assay enables high-throughput detection by flow cytometry of up to 65 microRNA targets and streamlined analysis with included FirePlex® Analysis Workbench software.
Our multiplex miRNA assays use our FirePlex® particle technology to profile up to 65 miRNA directly from 10 µL of biofluid, without the need for RNA purification. The assay provides PCR sensitivity while eliminating the need for separate reverse transcription reactions and mitigating amplification biases introduced by target-specific qPCR. This is made possible by combining uniquely encoded FirePlex® hydrogel particles with single-step RT-PCR signal amplification using universal primers. The entire assay is performed in 96-well plate format such that users detect up to 65 microRNA targets for each of 96 samples in less than a day’s work.
The FirePlex® miRNA Assay reliably detects as few as 1000 microRNA copies per sample with a linear dynamic range of ~5 logs. In addition to increased sensitivity, the assay's multiplexed detection conserves precious sample by detecting multiple microRNA targets from only 10 µL of biofluid, and has been validated for numerous sample types including plasma, serum, exosomes, urine and saliva.
For a full list of validated sample types, and to learn more about the performance and requirements of these assays, visit https://www.abcam.com/nav/multiplex-mirna-assays.
This product is currently only supported for customers in North America and Europe, if you are outside these areas please contact us.
FirePlex® is a registered trade mark in the United States and is an unregistered trade mark elsewhere.
|Components||1 x 96 tests|
|10x Rinse Buffer A||1 x 30ml|
|1x Hyb Buffer||1 x 25ml|
|5x Reporter Solution||1 x 4ml|
|Filter Plate||2 units|
|Catch Plate||2 units|
|RNA Control||1 x 50µl|
|Digest Buffer||1 x 4ml|
|dNTP mix||1 x 120µl|
|50x Labeling Buffer||1 x 200µl|
|Labeling Diluent||1 x 30ml|
|Labeling Enzyme||1 x 50µl|
|PCR Buffer||1 x 2ml|
|PCR Enzyme||1 x 60µl|
|Primer Mix||1 x 250µl|
|Protease Mix||1 x 500µl|
|2x Rinse Buffer B||1 x 33ml|
|Run buffer||1 x 30ml|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"