Product nameFirePlex® miRNA Assay Core Reagent Kit V2 - FFPE and Tissues
The FirePlex® miRNA Assay enables high-throughput detection by flow cytometry of up to 65 microRNA targets and streamlined analysis with included FirePlex® Analysis Workbench software.
Our multiplex miRNA assays use our FirePlex® particle technology to profile up to 65 miRNA directly from 5 micron sections of FFPE or fresh-frozen tissue, without the need for RNA purification. The assay provides PCR sensitivity while eliminating the need for separate reverse transcription reactions and mitigating amplification biases introduced by target-specific qPCR. This is made possible by combining uniquely encoded FirePlex® hydrogel particles with single-step RT-PCR signal amplification using universal primers. The entire assay is performed in 96-well plate format such that users detect up to 65 microRNA targets for each of 96 samples in less than a day’s work.
The FirePlex® miRNA Assay reliably detects as few as 1000 microRNA copies per sample with a linear dynamic range of ~5 logs. In addition to increased sensitivity, the assay's multiplexed detection conserves precious sample by detecting multiple microRNA targets from only 5 micron of tissue sample.
For a full list of validated sample types, and to learn more about the performance and requirements of these assays, visit https://www.abcam.com/nav/multiplex-assays/fireplex-mirna-assays
FirePlex® is a registered trade mark in the United States and is an unregistered trade mark elsewhere.
Storage instructionsPlease refer to protocols.
Components 1 x 96 tests 10x Rinse Buffer A 1 x 30ml 1x Hyb Buffer (Hybridization Buffer) 1 x 25ml 2x Rinse Buffer B 1 x 33ml 50x Labeling Buffer 1 x 200µl 5x Reporter Solution 1 x 4ml Catch Plate 2 units Digest Buffer 1 x 30ml dNTP mix 1 x 120µl FFPE/Tissue Control 1 unit Filter Plate 2 units Labeling Diluent 1 x 30ml Labeling Enzyme 1 x 50µl PCR Buffer 1 x 2ml PCR Enzyme 1 x 60µl Primer Mix 1 x 250µl Protease Mix 1 x 2ml RNA Control 1 x 50µl
- Vacuum manifold for 96 well filter plates (ab204067)
- FirePlex® miRNA Assay Core Reagent Kit V2 - purified RNA (ab218365)
- FirePlex miRNA Panel - Oncology V2 (ab218367)
- FirePlex® miRNA Panel - Cardiology V2 (ab218368)
- FirePlex® miRNA Panel - Immunology V2 (ab218369)
- FirePlex® miRNA Panel - Liver Tox V2 (ab218370)
- FirePlex® miRNA Panel - Neurology V2 (ab218371)
- FirePlex miRNA Panel - Cardiac Toxicity (ab219509)
- FirePlex miRNA Panel - Pancreatic Toxicity (ab219510)
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235025 has not yet been referenced specifically in any publications.