FirePlex® miRNA Assay Core Reagent Kit V2 - FFPE and Tissues (ab235025)

Overview

  • Product name
    FirePlex® miRNA Assay Core Reagent Kit V2 - FFPE and Tissues
  • Product overview

    The FirePlex® miRNA Assay enables high-throughput detection by flow cytometry of up to 65 microRNA targets and streamlined analysis with included FirePlex® Analysis Workbench software.


    Our multiplex miRNA assays use our FirePlex® particle technology to profile up to 65 miRNA directly from 5 micron sections of FFPE or fresh-frozen tissue, without the need for RNA purification. The assay provides PCR sensitivity while eliminating the need for separate reverse transcription reactions and mitigating amplification biases introduced by target-specific qPCR. This is made possible by combining uniquely encoded FirePlex® hydrogel particles with single-step RT-PCR signal amplification using universal primers. The entire assay is performed in 96-well plate format such that users detect up to 65 microRNA targets for each of 96 samples in less than a day’s work.


    The FirePlex® miRNA Assay reliably detects as few as 1000 microRNA copies per sample with a linear dynamic range of ~5 logs. In addition to increased sensitivity, the assay's multiplexed detection conserves precious sample by detecting multiple microRNA targets from only 5 micron of tissue sample.


    For a full list of validated sample types, and to learn more about the performance and requirements of these assays, visit https://www.abcam.com/nav/multiplex-assays/fireplex-mirna-assays  

  • Notes

     

     

     

     

     

     

    FirePlex® is a registered trade mark in the United States and is an unregistered trade mark elsewhere.

Properties

  • Storage instructions
    Please refer to protocols.
  • Components 1 x 96 tests
    10x Rinse Buffer A 1 x 30ml
    1x Hyb Buffer  1 x 25ml
    2x Rinse Buffer B 1 x 33ml
    50x Labeling Buffer 1 x 200µl
    5x Reporter Solution 1 x 4ml
    Catch Plate 2 units
    Digest Buffer 1 x 30ml
    dNTP mix 1 x 120µl
    FFPE/Tissue Control 1 unit
    Filter Plate 2 units
    Labeling Diluent 1 x 30ml
    Labeling Enzyme 1 x 50µl
    PCR Buffer 1 x 2ml
    PCR Enzyme 1 x 60µl
    Primer Mix 1 x 250µl
    Protease Mix 1 x 2ml
    RNA Control 1 x 50µl

References

ab235025 has not yet been referenced specifically in any publications.

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