FirePlex® miRNA Assay Core Reagent Kit V2 - purified RNA (ab218365)

Overview

  • Product name
    FirePlex® miRNA Assay Core Reagent Kit V2 - purified RNA
  • Detection method
    Flow cytometry-fluorescent
  • Assay type
    Multiplex
  • Assay time
    4h 00m
  • Product overview

    "FirePlex® miRNA Assay Core Reagent Kit V2 - purified RNA (ab218365) contains qualified reagents for the use of our FirePlex® miRNA multiplex assays which detect up to 65 literature curated miRNA in 10 µL of biofluid samples or 100 pg purified total RNA, without the need for RNA purification. FirePlex® miRNA Assay Core Reagent Kit V2 - Biofluid (ab218342) requires the purchase of either:


    - Oncology miRNA Panel (ab218367)
    - Cardiology miRNA Panel (ab218368)
    - Immunology miRNA Panel (ab218369)
    - Liver Tox miRNA Panel (ab218370)
    - Neurology miRNA Panel (ab218371)
    - Kidney Toxicity miRNA Panel (ab219508)
    - Cardiac Toxicity miRNA Panel (ab219509)
    - Pancreatic Toxicity miRNA Panel (ab219510)


    NOTE: FirePlex miRNA panels require the purchase of a Run Buffer. Use our FirePlex Cytometer Setup Kit V2 (ab245835) to select the most appropriate Run Buffer for your flow cytometer.


    The assay provides PCR sensitivity while eliminating the need for separate reverse transcription reactions and mitigating amplification biases introduced by target-specific qPCR. This is made possible by combining uniquely encoded FirePlex® hydrogel particles with single-step RT-PCR signal amplification using universal primers. The entire assay is performed in 96-well plate format such that users detect up to 65 microRNA targets for each of 96 samples in less than a day’s work.


    The FirePlex® miRNA Assay reliably detects as few as 1000 microRNA copies per sample with a linear dynamic range of ~5 logs. In addition to increased sensitivity, the assay's multiplexed detection conserves precious sample by detecting multiple microRNA targets from only 10 µL of biofluid, and has been validated for numerous sample types including plasma, serum, exosomes, urine and saliva.


    Find a full list of validated sample types, and learn more about the performance and requirements of these assays, here.


    Our integrated free FirePlex Analysis Workbench software enables easy and rapid data analysis, visualization, and export in under ten minutes, and includes key features such as standard curve analysis and publication-quality heat maps and graphs.

  • Notes

    FirePlex® is a registered trade mark in the United States and is an unregistered trade mark elsewhere.

  • Platform
    Multiplex

Properties

  • Storage instructions
    Please refer to protocols.
  • Components 1 x 96 tests
    10x Rinse Buffer A 1 x 30ml
    1x Hyb Buffer (Hybridization Buffer) 1 x 25ml
    5x Reporter Solution 1 x 4ml
    96 well Filter Plate 1 unit
    Catch Plate 1 unit
    dNTP mix 1 x 120µl
    FirePlex Plate Seal 1 unit
    Labeling Buffer 1 x 200µl
    Labeling Diluent 1 x 30ml
    Labeling Enzyme 1 x 50µl
    PCR Buffer 1 x 2ml
    PCR Enzyme 1 x 60µl
    Primer Mix 1 x 250µl
    Rinse Buffer B 1 x 33ml
    RNA Control 1 x 50µl

References

This product has been referenced in:
  • Hoss AG  et al. miR-10b-5p expression in Huntington's disease brain relates to age of onset and the extent of striatal involvement. BMC Med Genomics 8:10 (2015). Read more (PubMed: 25889241) »
  • Le MT  et al. miR-200-containing extracellular vesicles promote breast cancer cell metastasis. J Clin Invest 124:5109-28 (2014). Read more (PubMed: 25401471) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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