Key features and details
- Rabbit polyclonal to FIST
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Dog, Human, Chimpanzee
- Isotype: IgG
Product nameAnti-FIST antibody
See all FIST primary antibodies
DescriptionRabbit polyclonal to FIST
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Dog, Human, Chimpanzee
Synthetic peptide corresponding to Human FIST.
Database link: NM_005734
Previously labelled as HIPK3.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.05% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).|
|IHC-P||Use a concentration of 10 µg/ml.|
FunctionSeems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors.
Tissue specificityOverexpressed in multidrug resistant cells. Highly expressed in heart and skeletal muscle, and at lower levels in placenta, pancreas, brain, spleen, prostate, thymus, testis, small intestine, colon and leukocytes. Not found in liver and lung.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
Contains 1 protein kinase domain.
modificationsAutophosphorylated, but autophosphorylation is not required for catalytic activity.
May be sumoylated.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Androgen receptor interacting nuclear protein kinase antibody
- Androgen receptor-interacting nuclear protein kinase antibody
- ANPK antibody
ab72538 at 10µg/ml staining FIST in human testis by Immunohistochemistry using paraffin-embedded tissue.
All lanes : Anti-FIST antibody (ab72538) at 1 µg/ml
Lane 1 : Mouse testis lysate
Lane 2 : Mouse testis lysate with immunising peptide
Lane 3 : Rat testis lysate
Predicted band size: 134 kDa
Observed band size: 134 kDa
Additional bands at: 100 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
ab72538 has been referenced in 1 publication.
- Huh JH et al. Dysregulation of miR-106a and miR-591 confers paclitaxel resistance to ovarian cancer. Br J Cancer 109:452-61 (2013). PubMed: 23807165