• Product name

  • Description

    Rabbit polyclonal to FIST
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Dog, Human, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human FIST.
    Database link: NM_005734

  • Positive control

    • Mouse and rat testis lysates. Human testis tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7
  • General notes

    Previously labelled as HIPK3.



Our Abpromise guarantee covers the use of ab72538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).
IHC-P Use a concentration of 10 µg/ml.


  • Function

    Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors.
  • Tissue specificity

    Overexpressed in multidrug resistant cells. Highly expressed in heart and skeletal muscle, and at lower levels in placenta, pancreas, brain, spleen, prostate, thymus, testis, small intestine, colon and leukocytes. Not found in liver and lung.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational

    Autophosphorylated, but autophosphorylation is not required for catalytic activity.
    May be sumoylated.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Androgen receptor interacting nuclear protein kinase antibody
    • Androgen receptor-interacting nuclear protein kinase antibody
    • ANPK antibody
    • DYRK6 antibody
    • Fas interacting serine/threonine protein kinase antibody
    • Fas-interacting serine/threonine-protein kinase antibody
    • FIST antibody
    • FIST3 antibody
    • Hipk3 antibody
    • HIPK3_HUMAN antibody
    • homeodomain interacting protein kinase 3 antibody
    • Homeodomain-interacting protein kinase 3 antibody
    • Homolog of protein kinase YAK1 antibody
    • PKY antibody
    • RP24 297H17.5 antibody
    • YAK1 antibody
    see all


  • ab72538 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab72538 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab72538 at 10µg/ml staining FIST in human testis by Immunohistochemistry using paraffin-embedded tissue.

  • All lanes : Anti-FIST antibody (ab72538) at 1 µg/ml

    Lane 1 : Mouse testis lysate
    Lane 2 : Mouse testis lysate with immunising peptide
    Lane 3 : Rat testis lysate

    Predicted band size: 134 kDa
    Observed band size: 134 kDa
    Additional bands at: 100 kDa, 50 kDa. We are unsure as to the identity of these extra bands.


This product has been referenced in:

  • Huh JH  et al. Dysregulation of miR-106a and miR-591 confers paclitaxel resistance to ovarian cancer. Br J Cancer 109:452-61 (2013). Read more (PubMed: 23807165) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab72538.
Please use the links above to contact us or submit feedback about this product.

For licensing inquiries, please contact partnerships@abcam.com

Sign up