FITC Anti-2B4 antibody [244F4] (ab95805)
![Flow Cytometry - FITC Anti-2B4 antibody [244F4] (ab95805)](https://www.abcam.com/ps/products/95/ab95805/Images/ab95805-110048-anti-2b4-antibody-244f4-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-2B4 antibody [244F4] (ab95805)")
Key features and details
- FITC Rat monoclonal [244F4] to 2B4
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Overview
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Product name
FITC Anti-2B4 antibody [244F4]
See all 2B4 primary antibodies -
Description
FITC Rat monoclonal [244F4] to 2B4 -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse 2B4.
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Positive control
- BALB/c splenocytes.
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General notes
ab95805 was generated against cell lines expressing CD244. It reacts with the C57BL/6 but not Balb/c due to polymorphisms present in the immunoglobulin V domain of the protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: 0.87% Sodium chloride -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
ab95805 was generated against cell lines expressing CD244. It reacts with the C57BL/6 but not Balb/c due to polymorphisms present in the immunoglobulin V domain of the protein. -
Clonality
Monoclonal -
Clone number
244F4 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab95805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.25µg for 105-8 cells. Use final volume of 100µl.
ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Modulate other receptor-ligand interactions to enhance leukocyte activation. -
Tissue specificity
Expressed in spleen, PBL, followed by lung, liver, testis and small intestine. Expressed not only in NK cells, but also on monocytes and basophils. -
Sequence similarities
Contains 2 Ig-like (immunoglobulin-like) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 18106 Mouse
- SwissProt: Q07763 Mouse
- Unigene: 2299 Mouse
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Alternative names
- 2B4 antibody
- C9.1 antibody
- CD244 antibody
see all
Images
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Flow Cytometry - FITC Anti-2B4 antibody [244F4] (ab95805)
Staining of BALB/c splenocytes with Anti-NK1-1 PE and 0.125 µg of Rat IgG2a Isotype Control FITC (left) or 0.125 µg of ab95805 (right). Cells in the lymphocyte gate were used for analysis.
Datasheets and documents
References (0)
ab95805 has not yet been referenced specifically in any publications.
