FITC Anti-2B4 antibody [244F4] (ab95805)
![Flow Cytometry - FITC Anti-2B4 antibody [244F4] (ab95805)](https://www.abcam.com/ps/products/95/ab95805/Images/ab95805-110048-anti-2b4-antibody-244f4-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-2B4 antibody [244F4] (ab95805)")
Key features and details
- FITC Rat monoclonal [244F4] to 2B4
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Overview
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Product name
FITC Anti-2B4 antibody [244F4]
See all 2B4 primary antibodies -
Description
FITC Rat monoclonal [244F4] to 2B4 -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse 2B4.
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Positive control
- BALB/c splenocytes.
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General notes
ab95805 was generated against cell lines expressing CD244. It reacts with the C57BL/6 but not Balb/c due to polymorphisms present in the immunoglobulin V domain of the protein.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: 0.87% Sodium chloride -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
ab95805 was generated against cell lines expressing CD244. It reacts with the C57BL/6 but not Balb/c due to polymorphisms present in the immunoglobulin V domain of the protein. -
Clonality
Monoclonal -
Clone number
244F4 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 0.25µg for 105-8 cells.
Use final volume of 100µl.
ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
Flow Cyt
Use 0.25µg for 105-8 cells. Use final volume of 100µl.
ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Modulate other receptor-ligand interactions to enhance leukocyte activation. -
Tissue specificity
Expressed in spleen, PBL, followed by lung, liver, testis and small intestine. Expressed not only in NK cells, but also on monocytes and basophils. -
Sequence similarities
Contains 2 Ig-like (immunoglobulin-like) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 18106 Mouse
- SwissProt: Q07763 Mouse
- Unigene: 2299 Mouse
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Alternative names
- 2B4 antibody
- C9.1 antibody
- CD244 antibody
see all
Images
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Flow Cytometry - FITC Anti-2B4 antibody [244F4] (ab95805)
Staining of BALB/c splenocytes with Anti-NK1-1 PE and 0.125 µg of Rat IgG2a Isotype Control FITC (left) or 0.125 µg of ab95805 (right). Cells in the lymphocyte gate were used for analysis.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab95805 has not yet been referenced specifically in any publications.