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  1. Link

    fitc-6x-his-tag-antibody-ab1206.pdf

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Tags & Cell Markers Epitope Tags His Tag
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FITC Anti-6X His tag® antibody (ab1206)

  • Datasheet
  • SDS
Reviews (7)Q&A (2)References (19)

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Abpromise

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Flow Cytometry - FITC Anti-6X His tag® antibody (ab1206)
  • Flow Cytometry - FITC Anti-6X His tag® antibody (ab1206)

Key features and details

  • FITC Rabbit polyclonal to 6X His tag®
  • Suitable for: ELISA, Flow Cyt
  • Reacts with: Species independent
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

Overview

  • Product name

    FITC Anti-6X His tag® antibody
    See all 6X His tag® primary antibodies
  • Description

    FITC Rabbit polyclonal to 6X His tag®
  • Host species

    Rabbit
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: ELISA, Flow Cytmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide: HHHHHH conjugated to KLH.

  • General notes

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    Molar F/P ratio is 3.7:1.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • His Tag

Associated products

  • Isotype control

    • FITC-Rabbit IgG - Isotype Control (ab37406)

Applications

Our Abpromise guarantee covers the use of ab1206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Relevance

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization

    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Images

  • Flow Cytometry - FITC Anti-6X His tag® antibody (ab1206)
    Flow Cytometry - FITC Anti-6X His tag® antibody (ab1206)Li et al PLoS One. 2018 Jan 22;13(1):e0191024. doi: 10.1371/journal.pone.0191024. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Muc1-Bi-1 and Muc1-Bi-2 can recognize Muc1 positive tumor cells.

    a). Flow cytometry analysis of Muc1-Bi-1 and Muc1-Bi-2 on Muc1-negative cell lines HePG2 and CHO and Muc1-positive cell lines, HT29. Red line indicates cells with no staining; green line indicates cells with only anti-His-FITC staining (ab1206); blue line indicates cells with positive control anti-Muc1 antibody; purple line indicates cells with Muc1-Bi-1 protein; orange line indicates cells with Muc1-Bi-2 protein.

  • Flow Cytometry - FITC Anti-6X His tag® antibody (ab1206)
    Flow Cytometry - FITC Anti-6X His tag® antibody (ab1206)This image is courtesy of an anonymous Abreview

    ab1206 staining 6X His tag® in human endometrial carcinoma cells by Flow Cytometry.

    Cells were prepared by short trypsinisation in PBS/1% FBS/0.2% sodium azide and fixed with formaldehyde. The gate included all single cells. The sample was incubated with  a 6X His tag® primary antibody for 1 hour at 4°C. ab1206 was used as the secondary. On the image, the red line represents cells stained with ab1206 alone, and the blue line is the tested sample carrying a 6XHis tag®.  The small peak to the right represents cells that bound the tested sample.

    See Abreview

Protocols

  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (19)

    Publishing research using ab1206? Please let us know so that we can cite the reference in this datasheet.

    ab1206 has been referenced in 19 publications.

    • Wang J  et al. In vitro affinity maturation of antibody against membrane-bound GPCR molecules. Appl Microbiol Biotechnol 103:7703-7717 (2019). PubMed: 31359103
    • Liu Z  et al. Development of two antigen-binding fragments to a conserved linear epitope of human adenovirus and their application in immunofluorescence. PLoS One 14:e0219091 (2019). PubMed: 31242267
    • Sang B  et al. Ras-AKT signaling represses the phosphorylation of histone H1.5 at threonine 10 via GSK3 to promote the progression of glioma. Artif Cells Nanomed Biotechnol 47:2882-2890 (2019). PubMed: 31307224
    • Wang H  et al. Rice Yellow Stunt Nucleorhabdovirus Matrix Protein Mediates Viral Axonal Transport in the Central Nervous System of Its Insect Vector. Front Microbiol 10:939 (2019). PubMed: 31143161
    • Case BA  et al. Evaluation of affibody charge modification identified by synthetic consensus design in molecular PET imaging of epidermal growth factor receptor. Mol Syst Des Eng 3:171-182 (2018). PubMed: 31467687
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-9 of 9 Abreviews or Q&A

    Western blot abreview for Anti-6X His tag® antibody (FITC)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Fall armyworm Recombinant protein (SF21 insect cells)
    Gel Running Conditions
    Reduced Denaturing (10% SDS PAGE)
    Loading amount
    10 µg
    Specification
    SF21 insect cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 20°C
    Read More

    Ms. Samira Acajjaoui

    Verified customer

    Submitted Jan 28 2019

    Flow Cytometry abreview for Anti-6X His tag® antibody (FITC)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Spodoptera frugiperda (SF9 cells) Cell (Baculovirus infected-cells. Baculovirus having a f)
    Permeabilization
    Yes - Saponin
    Gating Strategy
    Single cells (FS-A vs FS-H) then SS-A vs FS-A
    Specification
    Baculovirus infected-cells. Baculovirus having a f
    Preparation
    Cell harvesting/tissue preparation method: Cell culture gently pipetting up and down
    Sample buffer: Pelleted in their own culture medium
    Fixation
    Formaldehyde
    Read More

    Dr. Waldo Medina

    Verified customer

    Submitted May 02 2016

    Flow Cytometry abreview for Anti-6X His tag® antibody (FITC)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Detection of a HIS tagged protein bound to cells)
    Specification
    Detection of a HIS tagged protein bound to cells
    Preparation
    Cell harvesting/tissue preparation method: Trypsinisation and pre-incubation with HIS-tagged protein
    Sample buffer: Serum free DMEM
    Fixation
    Nonne
    Permeabilization
    No
    Gating Strategy
    Live cell population
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 02 2012

    Question

    Phoned looking for a capture antibody, protein, detection antibody and FITC conjugation kit for Dengue-Virus-NS1-glycoprotein.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 07 2012

    Answer

    I've assembled a list of the items that you would need forDengue Virus NS1 glycoprotein detection. Capture antibody ab41646,Anti-Dengue Virus NS1 glycoprotein antibody [DN3] Mouse monoclonal: https://www.abcam.com/Dengue-Virus-NS1-glycoprotein-antibody-DN3-ab41616.html Dengue Virus NS1 glycoproteinProtein, ab64456. This is a His tagged protein which allows for a greater selection of detection antibodies and thereby give you options for detection substrates: https://www.abcam.com/Dengue-Virus-NS1-glycoprotein-protein-His-tag-ab64456.html Detection antibody. These are all rabbit polyclonal with differing labels that you might use with different detection systems: FITC labeled:https://www.abcam.com/6X-His-tag-antibody-FITC-ab1206.html HRP labeled:https://www.abcam.com/6X-His-tag-antibody-HRP-ab1187.html, you would need to purchase an HRP chromogen substrate for detection using this. Biotin labeled:https://www.abcam.com/6X-His-tag-antibody-Biotin-ab27025.html,you would need to purchase a streptavidin conjugated chromogen substrate system for detection using this. You may want to use ab41646, the 'capture' antibody for 'detection' instead. To do that you will need to conjugate a tag to the antibody. We have a number of products available for that. Here is the link to all of our EasyLink Conjugation kits: https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink A link to the FAQs for EasyLink, please review these as they contain very helpful information:https://www.abcam.com/index.html?pageconfig=resource&rid=13156 We offer 3 sizes for FITC conjugation, I've linked those below: https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-3-x-10%C2%B5g-FITC-ab102885.html https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-3-x-100%C2%B5g-FITC-ab102884.html https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-3-x-100%C2%B5g-FITC-ab102884.html

    Read More

    Abcam Scientific Support

    Answered on Mar 07 2012

    Flow Cytometry abreview for Anti-6X His tag® antibody (FITC)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (A431)
    Specification
    A431
    Preparation
    Cell harvesting/tissue preparation method: trypsin/EDTA
    Sample buffer: PBS + 1 g/L BSA
    Fixation
    none
    Permeabilization
    No
    Gating Strategy
    no gating
    Read More

    Dr. Benjamin Hackel

    Verified customer

    Submitted Sep 14 2010

    Flow Cytometry abreview for Anti-6X His tag® antibody (FITC)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (endometrial carcinoma)
    Specification
    endometrial carcinoma
    Preparation
    Cell harvesting/tissue preparation method: short trypsinisation
    Sample buffer: PBS+1%FBS+0.02%NaN3
    Fixation
    Formaldehyde
    Permeabilization
    No
    Gating Strategy
    gate included all single cells
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 07 2009

    ELISA abreview for Anti-6X His tag® antibody (FITC)

    Good
    Abreviews
    Abreviews
    Application
    ELISA
    Sample
    Pichia pastoris Recombinant protein (yeast cell)
    Specification
    yeast cell
    Blocking step
    BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Type
    Indirect
    Read More

    Abcam user community

    Verified customer

    Submitted May 14 2009

    Flow Cytometry abreview for Anti-6X His tag® antibody (FITC)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Saccharomyces cerevisiae Cell (yeast cells expressing a his tag on surface)
    Specification
    yeast cells expressing a his tag on surface
    Permeabilization
    No
    Gating Strategy
    gate included all yeast, both expressing and non-expressing
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 04 2008

    Question

    Customer would like to know the Molecular Weight.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 03 2004

    Answer

    The molecular weight of Rabbit IgG is ~150,000.

    Read More

    Abcam Scientific Support

    Answered on Sep 22 2004

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