FITC Anti-6X His tag® antibody (ab1206)
Key features and details
- FITC Rabbit polyclonal to 6X His tag®
- Suitable for: ELISA, Flow Cyt
- Reacts with: Species independent
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Overview
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Product name
FITC Anti-6X His tag® antibody
See all 6X His tag® primary antibodies -
Description
FITC Rabbit polyclonal to 6X His tag® -
Host species
Rabbit -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: ELISA, Flow Cytmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide: HHHHHH conjugated to KLH.
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General notes
HIS-TAG® is a trademark of EMD Biosciences, Inc.
Molar F/P ratio is 3.7:1.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1206 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | (1) |
Use at an assay dependent concentration.
|
| Flow Cyt | (5) |
Use at an assay dependent concentration.
ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Images
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Flow Cytometry - FITC Anti-6X His tag® antibody (ab1206)Li et al PLoS One. 2018 Jan 22;13(1):e0191024. doi: 10.1371/journal.pone.0191024. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Muc1-Bi-1 and Muc1-Bi-2 can recognize Muc1 positive tumor cells.
a). Flow cytometry analysis of Muc1-Bi-1 and Muc1-Bi-2 on Muc1-negative cell lines HePG2 and CHO and Muc1-positive cell lines, HT29. Red line indicates cells with no staining; green line indicates cells with only anti-His-FITC staining (ab1206); blue line indicates cells with positive control anti-Muc1 antibody; purple line indicates cells with Muc1-Bi-1 protein; orange line indicates cells with Muc1-Bi-2 protein.
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Flow Cytometry - FITC Anti-6X His tag® antibody (ab1206)This image is courtesy of an anonymous Abreviewab1206 staining 6X His tag® in human endometrial carcinoma cells by Flow Cytometry.
Cells were prepared by short trypsinisation in PBS/1% FBS/0.2% sodium azide and fixed with formaldehyde. The gate included all single cells. The sample was incubated with a 6X His tag® primary antibody for 1 hour at 4°C. ab1206 was used as the secondary. On the image, the red line represents cells stained with ab1206 alone, and the blue line is the tested sample carrying a 6XHis tag®. The small peak to the right represents cells that bound the tested sample.
Datasheets and documents
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SDS download
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Datasheet download
References (22)
ab1206 has been referenced in 22 publications.
- Zhang C et al. Novel EGFR-bispecific recombinant immunotoxin based on cucurmosin shows potent anti-tumor efficiency in vitro. Oncol Rep N/A:N/A (2020). PubMed: 33416157
- Du F et al. Engineering an EGFR-binding Gp2 domain for increased hydrophilicity. Biotechnol Bioeng 116:526-535 (2019). PubMed: 30536855
- Stern LA et al. Cellular-Based Selections Aid Yeast-Display Discovery of Genuine Cell-Binding Ligands: Targeting Oncology Vascular Biomarker CD276. ACS Comb Sci 21:207-222 (2019). PubMed: 30620189
- Wang H et al. Rice Yellow Stunt Nucleorhabdovirus Matrix Protein Mediates Viral Axonal Transport in the Central Nervous System of Its Insect Vector. Front Microbiol 10:939 (2019). PubMed: 31143161
- Wang J et al. In vitro affinity maturation of antibody against membrane-bound GPCR molecules. Appl Microbiol Biotechnol 103:7703-7717 (2019). PubMed: 31359103