FITC Anti-6X His tag® antibody (ab1270)

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Key features and details

  • FITC Goat polyclonal to 6X His tag®
  • Suitable for: ICC
  • Reacts with: Species independent
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

Overview

  • Product name

    FITC Anti-6X His tag® antibody
    See all 6X His tag® primary antibodies

  • Description

    FITC Goat polyclonal to 6X His tag®

  • Host species

    Goat

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Tested applications

    Suitable for: ICCmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide:

    HHHHHH

    (6-His) conjugated to KLH.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: 0.2% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Associated products

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab1270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
1/200 - 1/2000.
Notes
ICC
1/200 - 1/2000.

Target

  • Relevance

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

  • Cellular localization

    Depends upon the localization of the parent protein tagged with hexahistidine.

  • Alternative names

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References (1)

Publishing research using ab1270? Please let us know so that we can cite the reference in this datasheet.

ab1270 has been referenced in 1 publication.

  • Tai-Nagara I  et al. Placental labyrinth formation in mice requires endothelial FLRT2/UNC5B signaling. Development 144:2392-2401 (2017). PubMed: 28576770

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Customer woul like to know: Does ab1270 react with the N terminus or C terminus end of 6-His?

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Answer

The antibody is designed to react with 6 His at the N-terminus and C-terminus.

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Question

I was wondering about the fixatives used for this antibody. Is paraformaldehyde ok, or must I use methanol, etc. More importantly, can this antibody be used on unfixed cells to detect the presence of a cell-surface HIS-tagged protein in unpermeabilized cells ?

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Answer

The method of fixation is unlikely to have significant effect on the His-tag. However, the fixation may influence the conformation of the tagged protein, and thereby, affect the accessibility of the His-tag. Thus, one must empirically determine the influence of fixation upon ability to detect tagged proteins.

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