FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab64503)
![Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab64503)](https://www.abcam.com/ps/products/64/ab64503/Images/ab7291-IF-Im4.jpg "Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab64503)")
Key features and details
- FITC Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
Overview
-
Product name
FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker
See all alpha Tubulin primary antibodies -
Description
FITC Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Gerbil
-
Immunogen
Full length native protein (purified) corresponding to alpha Tubulin.
-
Epitope
aa426-450. Ab64503 specifically recognizes an epitope in the carboxy-terminal part of alpha-tubulin. -
Positive control
- ICC/IF: HeLa cells. IHC-Fr: Xenopus laevis stage 36 tissue. Flow cyt: HeLa cells. Cultured human fibroblasts, baby hamster kidney (BHK) cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
DM1A -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
- Alexa Fluor® 680 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184093)
- Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184577)
- Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)
- Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
- Anti-alpha Tubulin antibody [DM1A] - BSA and Azide free (ab264493)
- HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742)
- Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 5.1µl for 106 cells.
ab106163 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
|
| ICC/IF | (4) |
Use a concentration of 1 µg/ml.
|
| Notes |
|---|
|
Flow Cyt
Use 5.1µl for 106 cells. ab106163 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
|
ICC/IF
Use a concentration of 1 µg/ml. |
Target
-
Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
Sequence similarities
Belongs to the tubulin family. -
Post-translational
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
-
Database links
- Entrez Gene: 7277 Human
- Entrez Gene: 22145 Mouse
- Entrez Gene: 316531 Rat
- Omim: 191110 Human
- SwissProt: P68366 Human
- SwissProt: P68368 Mouse
- SwissProt: Q5XIF6 Rat
- Unigene: 75318 Human
see all -
Alternative names
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab64503)ICC/IF image of ab64503 stained human HeLa cells. The cells were methanol fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab64503, 1µg/ml, FITC conjugated (green)) for 1h at room temperature. 1% BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
-
![Flow Cytometry - FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab64503)](https://www.abcam.com/ps/products/64/ab64503/Images/alpha-Tubulin-Primary-antibodies-ab64503-6.jpg)
Flow Cytometry - FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab64503)Overlay histogram showing HeLa cells stained with ab64503 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab64503, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (1µg/1x106 cells ). Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
Datasheets and documents
-
SDS download
-
Datasheet download
References (16)
ab64503 has been referenced in 16 publications.
- Luo D et al. Imperatorin Ameliorates the Aging-Associated Porcine Oocyte Meiotic Spindle Defects by Reducing Oxidative Stress and Protecting Mitochondrial Function. Front Cell Dev Biol 8:592433 (2020). PubMed: 33409275
- Eck F et al. ACSL3 is a novel GABARAPL2 interactor that links ufmylation and lipid droplet biogenesis. J Cell Sci 133:N/A (2020). PubMed: 32843575
- Zheng N et al. Development of a Subcellular Semimechanism-Based Pharmacokinetic/Pharmacodynamic Model to Characterize Paclitaxel Effects Delivered by Polymeric Micelles. J Pharm Sci 108:725-731 (2019). PubMed: 30414867
- Zhou C et al. Substrate Compliance Directs the Osteogenic Lineages of Stem Cells from the Human Apical Papilla via the Processes of Mechanosensing and Mechanotransduction. ACS Appl Mater Interfaces 11:26448-26459 (2019). PubMed: 31251564
- Usuki S et al. Konjac Ceramide (kCer) Regulates NGF-Induced Neurite Outgrowth via the Sema3A Signaling Pathway. J Oleo Sci 67:77-86 (2018). PubMed: 29238028