Overview

  • Product name

    Anti-FITC antibody [7F4]
    See all FITC primary antibodies
  • Description

    Mouse monoclonal [7F4] to FITC
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Conjugate of Bovine serum albumin (BSA) and Fluorescein isothiocyanate (FITC).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C.
  • Storage buffer

    pH: 7.40
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity is > 95% by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    7F4
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab116449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 1 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Relevance

    FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
  • Alternative names

    • Fluorescein Isothiocyanate antibody

References

ab116449 has not yet been referenced specifically in any publications.

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