Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab19224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/1000 - 1/30000. Suggested coating dilutions: 1/100 - 1/100.|
|ICC||1/200 - 1/2000.|
|WB||Use at an assay dependent dilution. Colorimetric detection: 1/1000 - 1/10000, Chemiluminescent detection: 1/1000 - 1/30000.|
|IP||Use at an assay dependent dilution. PubMed: 17052454|
RelevanceFITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
- Fluorescein Isothiocyanate antibody
This product has been referenced in:
- Natoli ME et al. Paper-based detection of HIV-1 drug resistance using isothermal amplification and an oligonucleotide ligation assay. Anal Biochem 544:64-71 (2018). Read more (PubMed: 29229373) »
- Fu H et al. NIPAAm-MMA nanoparticle-encapsulated visnagin ameliorates myocardial ischemia/reperfusion injury through the promotion of autophagy and the inhibition of apoptosis. Oncol Lett 15:4827-4836 (2018). Read more (PubMed: 29552122) »