Overview

  • Product name
  • Description
    Goat polyclonal to FITC
  • Host species
    Goat
  • Tested applications
    Suitable for: ELISA, ICC, WB, IPmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to FITC conjugated to keyhole limpet haemocyanin.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/30000. Suggested coating dilutions: 1/100 - 1/100.
ICC 1/200 - 1/2000.
WB Use at an assay dependent dilution. Colorimetric detection: 1/1000 - 1/10000, Chemiluminescent detection: 1/1000 - 1/30000.
IP Use at an assay dependent dilution. PubMed: 17052454

Target

  • Relevance
    FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
  • Alternative names
    • Fluorescein Isothiocyanate antibody

References

This product has been referenced in:
  • Natoli ME  et al. Paper-based detection of HIV-1 drug resistance using isothermal amplification and an oligonucleotide ligation assay. Anal Biochem 544:64-71 (2018). Read more (PubMed: 29229373) »
  • Fu H  et al. NIPAAm-MMA nanoparticle-encapsulated visnagin ameliorates myocardial ischemia/reperfusion injury through the promotion of autophagy and the inhibition of apoptosis. Oncol Lett 15:4827-4836 (2018). Read more (PubMed: 29552122) »
See all 7 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Question
Answer

Thank you for contacting us.

The antibodies are from different animals and are manufactured in different laboratories so we can expect some variation. The product ab19224 is against FITC which is a derivative of Flouroscein.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you very much for your interest in ab19224. To our knowledge, ab19224 has not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buy ab19224 now, test it inIHC-Fr and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab19224 in IHC-Fr. I will then send a discount code. This code must be issued before purchasing ab19224 so please wait for my reply before ordering. 2. Purchase ab19224 either by phone, fax, or online (www.abcam.com). 3. Test it in IHC-Fr. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab19224 turns out to be unsuitable for IHC-Fr, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Answer

Thank you for your enquiry. Ab19224 reacts specifically with FITC and there is no reaction with KLH. The antibody has been characterized for application in Western blot, ELISA, and Immunocytochemistry. We don't have any images available at this time, but do guarantee that that this antibody will detect FITC in Western blotting, ELISA, and Immunocytochemistry. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. If you have any additional questions, please contact us again.

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Question
Answer

Thank you for your enquiry. It appears there was an error on the datasheet. This antibody has not been tested in IHC. I have updated the datasheet to reflect this, and I apologize for any inconvenience this may have caused. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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