Anti-FITC antibody (ab19224)
Key features and details
- Goat polyclonal to FITC
- Suitable for: IP, ELISA, ICC, WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-FITC antibody
See all FITC primary antibodies -
Description
Goat polyclonal to FITC -
Host species
Goat -
Tested applications
Suitable for: IP, ELISA, ICC, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to FITC conjugated to keyhole limpet haemocyanin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab19224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration. PubMed: 17052454
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ELISA |
1/1000 - 1/30000. Suggested coating dilutions: 1/100 - 1/100.
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ICC |
1/200 - 1/2000.
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WB |
Use at an assay dependent concentration. Colorimetric detection: 1/1000 - 1/10000, Chemiluminescent detection: 1/1000 - 1/30000.
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Notes |
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IP
Use at an assay dependent concentration. PubMed: 17052454 |
ELISA
1/1000 - 1/30000. Suggested coating dilutions: 1/100 - 1/100. |
ICC
1/200 - 1/2000. |
WB
Use at an assay dependent concentration. Colorimetric detection: 1/1000 - 1/10000, Chemiluminescent detection: 1/1000 - 1/30000. |
Target
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Relevance
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. -
Alternative names
- Fluorescein Isothiocyanate antibody
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab19224 has been referenced in 17 publications.
- Thomas R et al. Integrated regulation of PKA by fast and slow neurotransmission in the nucleus accumbens controls plasticity and stress responses. J Biol Chem 298:102245 (2022). PubMed: 35835216
- Aftabizadeh M et al. Potent antitumor effects of cell-penetrating peptides targeting STAT3 axis. JCI Insight 6:N/A (2021). PubMed: 33491667
- Krishnamoorthy G et al. Role of Premycofactocin Synthase in Growth, Microaerophilic Adaptation, and Metabolism of Mycobacterium tuberculosis. mBio 12:e0166521 (2021). PubMed: 34311585
- Krishnamoorthy G et al. FX11 limits Mycobacterium tuberculosis growth and potentiates bactericidal activity of isoniazid through host-directed activity. Dis Model Mech 13:N/A (2020). PubMed: 32034005
- Azevedo C et al. Development of a yeast model to study the contribution of vacuolar polyphosphate metabolism to lysine polyphosphorylation. J Biol Chem 295:1439-1451 (2020). PubMed: 31844018