Overview

  • Product name

    Anti-FITC antibody (HRP)
    See all FITC primary antibodies
  • Description

    Rabbit polyclonal to FITC (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Specificity

    ab196968 recognizes any FITC-labeled protein.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to FITC conjugated to Keyhole Limpet Haemocyanin (KLH).

  • General notes

    Conjugation: Reductive amination with peroxidase-oxidized HRP. HRP/Ab ratio=2:1.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Constituents: 50% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab196968 is affinity-purified with FITC-linked agarose.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab196968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance

    FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
  • Alternative names

    • Fluorescein Isothiocyanate antibody

References

ab196968 has not yet been referenced specifically in any publications.

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