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Recombinant

Recombinant FITC Anti-Aurora B + Aurora A (phospho T288) + IPL-1/STK13/Aurora C antibody [AuroraABC-CC12] (ab278776)

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Flow Cytometry - FITC Anti-Aurora B + Aurora A (phospho T288) + IPL-1/STK13/Aurora C antibody [AuroraABC-CC12] (ab278776)
  • FITC Anti-Aurora B + Aurora A (phospho T288) + IPL-1/STK13/Aurora C antibody [AuroraABC-CC12] (ab278776)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • FITC Rabbit monoclonal [AuroraABC-CC12] to Aurora B (phospho T232) + Aurora A (phospho T288) + IPL-1/STK13/Aurora C (phospho T198)
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm

Conjugates logo Related conjugates and formulations

APC PE Unconjugated

Overview

  • Product name

    FITC Anti-Aurora B + Aurora A (phospho T288) + IPL-1/STK13/Aurora C antibody [AuroraABC-CC12]

  • Description

    FITC Rabbit monoclonal [AuroraABC-CC12] to Aurora B (phospho T232) + Aurora A (phospho T288) + IPL-1/STK13/Aurora C (phospho T198)

  • Host species

    Rabbit

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide within Human Aurora A (phospho T228). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

    A synthetic phospho-peptide corresponding to residues surrounding human Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198)

    Uniprot O14965/ Q96GD4/ Q9UQB9
    Database link: O14965

    Synthetic peptide corresponding to Aurora B (phospho T232).
    Database link: Q96GD4

    Synthetic peptide corresponding to IPL-1/STK13/Aurora C (phospho T198).
    Database link: Q9UQB9
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Flow cyt: HeLa cells treated with nocodazole.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab278776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 5µl for 106 cells.
Notes
Flow Cyt
Use 5µl for 106 cells.

Target

  • Cellular localization

    Aurora B: Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body. Aurora A: Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis. IPL-1/STK13/Aurora C: Chromosome. Cytoplasm > cytoskeleton > spindle. Distributes in the condensed chromosomes during prophase to metaphase. After entering anaphase, there is a dissociation from separated chromosomes and a redistribution to midzone microtubules, and finally remaines in the midbody during cytokinesis.

  • Database links

    see all

Images

  • Flow Cytometry - FITC Anti-Aurora B + Aurora A (phospho T288) + IPL-1/STK13/Aurora C antibody [AuroraABC-CC12] (ab278776)
    Flow Cytometry - FITC Anti-Aurora B + Aurora A (phospho T288) + IPL-1/STK13/Aurora C antibody [AuroraABC-CC12] (ab278776)
    Flow cytometric analysis of HeLa cells untreated and unstained as negative control (blue) or untreated (red) or treated with nocodazole (green) and stained using ab278776.
  • FITC Anti-Aurora B + Aurora A (phospho T288) + IPL-1/STK13/Aurora C antibody [AuroraABC-CC12] (ab278776)
    FITC Anti-Aurora B + Aurora A (phospho T288) + IPL-1/STK13/Aurora C antibody [AuroraABC-CC12] (ab278776)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab278776? Please let us know so that we can cite the reference in this datasheet.

ab278776 has not yet been referenced specifically in any publications.

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