FITC Anti-beta Actin antibody [AC-15] (ab6277)
![Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)](https://www.abcam.com/ps/products/6/ab6277/Images/ab6277-12195-anti-beta-actin-antibody-ac-15-fitc-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)")
Key features and details
- FITC Mouse monoclonal [AC-15] to beta Actin
- Suitable for: ICC/IF
- Reacts with: Rat, Chicken
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
FITC Anti-beta Actin antibody [AC-15]
See all beta Actin primary antibodies -
Description
FITC Mouse monoclonal [AC-15] to beta Actin -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Rat, Chicken -
Immunogen
Synthetic peptide:
DDDIAALVIDNGSGL
conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta). -
Epitope
The N-terminal of the beta isoform of actin -
Positive control
- Cultured human or chicken fibroblasts. Molecular weight of beta actin is 42Kd.
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General notes
Immunofluoresence staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membrane associated dense-plaque.Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Purified Immunoglobulin -
Primary antibody notes
Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells -
Clonality
Monoclonal -
Clone number
AC-15 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 396526 Chicken
- Entrez Gene: 81822 Rat
- SwissProt: P60706 Chicken
- SwissProt: P60711 Rat
- Unigene: 94978 Rat
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Alternative names
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)
ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.
30 min blocking with TBS-T / 5% BSA
1 hr incubation with ab6277 (TBS-T / 5% BSA)
1 minute nuclear counterstaining with DAPIMagnification x20
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![Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)](https://www.abcam.com/ps/products/6/ab6277/Images/ab6277-274544-anti-beta-actin-antibody-ac-15-fitc-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)Immunocytochemistry/ Immunofluorescence analysis of chicken fibroblast cells labeling beta Actin with ab6277 at 1/300 dilution. The cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 1/300 Anti-beta Actin antibody [AC-15] (FITC) (ab6277).
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![Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)](https://www.abcam.com/ps/products/6/ab6277/Images/ab6277-12194-anti-beta-actin-antibody-ac-15-fitc-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.
30 min blocking with TBS-T / 5% BSA
1 hr incubation with ab6277 (TBS-T / 5% BSA)
1 minute nuclear counterstaining with DAPIMagnification x40 no deconvolution.
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab6277 has been referenced in 18 publications.
- Cagnetta R et al. Noncanonical Modulation of the eIF2 Pathway Controls an Increase in Local Translation during Neural Wiring. Mol Cell 73:474-489.e5 (2019). PubMed: 30595434
- Réu P et al. A 61% lighter cell culture dish to reduce plastic waste. PLoS One 14:e0216251 (2019). PubMed: 31039189
- Shekaramiz E et al. Protein fishing from single live cells. J Nanobiotechnology 16:67 (2018). PubMed: 30205820
- Cagnetta R et al. Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome. Neuron 99:29-46.e4 (2018). PubMed: 30008298
- Olthoff JT et al. Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle. Nat Commun 9:5104 (2018). PubMed: 30504831