FITC Anti-beta Actin antibody [AC-15] (ab6277)
![Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)](https://www.abcam.com/ps/products/6/ab6277/Images/ab6277-12195-anti-beta-actin-antibody-ac-15-fitc-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)")
Key features and details
- FITC Mouse monoclonal [AC-15] to beta Actin
- Suitable for: ICC/IF
- Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig, Drosophila melanogaster, Fish
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-beta Actin antibody [AC-15]
See all beta Actin primary antibodies -
Description
FITC Mouse monoclonal [AC-15] to beta Actin -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig, Drosophila melanogaster, Fish -
Immunogen
Synthetic peptide:
DDDIAALVIDNGSGL
conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta). -
Epitope
The N-terminal of the beta isoform of actin -
Positive control
- Cultured human or chicken fibroblasts. Molecular weight of beta actin is 42Kd.
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General notes
Immunofluoresence staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membrane associated dense-plaque.Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Purified Immunoglobulin -
Primary antibody notes
Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells -
Clonality
Monoclonal -
Clone number
AC-15 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab6277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 396526 Chicken
- Entrez Gene: 280979 Cow
- Entrez Gene: 403580 Dog
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 414396 Pig
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
see all -
Alternative names
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)
ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.
30 min blocking with TBS-T / 5% BSA
1 hr incubation with ab6277 (TBS-T / 5% BSA)
1 minute nuclear counterstaining with DAPIMagnification x20
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![Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)](https://www.abcam.com/ps/products/6/ab6277/Images/ab6277-274544-anti-beta-actin-antibody-ac-15-fitc-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)Immunocytochemistry/ Immunofluorescence analysis of chicken fibroblast cells labeling beta Actin with ab6277 at 1/300 dilution. The cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 1/300 Anti-beta Actin antibody [AC-15] (FITC) (ab6277).
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![Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)](https://www.abcam.com/ps/products/6/ab6277/Images/ab6277-12194-anti-beta-actin-antibody-ac-15-fitc-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.
30 min blocking with TBS-T / 5% BSA
1 hr incubation with ab6277 (TBS-T / 5% BSA)
1 minute nuclear counterstaining with DAPIMagnification x40 no deconvolution.
References (17)
ab6277 has been referenced in 17 publications.
- Cagnetta R et al. Noncanonical Modulation of the eIF2 Pathway Controls an Increase in Local Translation during Neural Wiring. Mol Cell 73:474-489.e5 (2019). PubMed: 30595434
- Réu P et al. A 61% lighter cell culture dish to reduce plastic waste. PLoS One 14:e0216251 (2019). PubMed: 31039189
- Cagnetta R et al. Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome. Neuron 99:29-46.e4 (2018). PubMed: 30008298
- Olthoff JT et al. Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle. Nat Commun 9:5104 (2018). PubMed: 30504831
- Llewellyn KJ et al. A Fine Balance of Dietary Lipids Improves Pathology of a Murine Model of VCP-Associated Multisystem Proteinopathy. PLoS One 10:e0131995 (2015). WB . PubMed: 26134519
