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  1. Link

    fitc-beta-actin-antibody-ac-15-ab6277.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Actin
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FITC Anti-beta Actin antibody [AC-15] (ab6277)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (17)

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Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)
  • Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)
  • Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)

Key features and details

  • FITC Mouse monoclonal [AC-15] to beta Actin
  • Suitable for: ICC/IF
  • Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig, Drosophila melanogaster, Fish
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG1

You may also be interested in

Protein
Product image
Recombinant Human beta Actin protein (His tag) (ab240844)

View more associated products

Overview

  • Product name

    FITC Anti-beta Actin antibody [AC-15]
    See all beta Actin primary antibodies
  • Description

    FITC Mouse monoclonal [AC-15] to beta Actin
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig, Drosophila melanogaster, Fish
  • Immunogen

    Synthetic peptide:

    DDDIAALVIDNGSGL

    conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Epitope

    The N-terminal of the beta isoform of actin
  • Positive control

    • Cultured human or chicken fibroblasts. Molecular weight of beta actin is 42Kd.
  • General notes

    Immunofluoresence staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membrane associated dense-plaque.


    Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    Purified Immunoglobulin
  • Primary antibody notes

    Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells
  • Clonality

    Monoclonal
  • Clone number

    AC-15
  • Isotype

    IgG1
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin

Associated products

  • Alternative Versions

    • HRP Anti-beta Actin antibody [AC-15] (ab49900)
    • Anti-beta Actin antibody [AC-15] (ab6276)
  • Isotype control

    • FITC Mouse IgG1 [B11/6] - Isotype Control (ab91356)
  • Recombinant Protein

    • Recombinant Human beta Actin protein (His tag) (ab240844)

Applications

Our Abpromise guarantee covers the use of ab6277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P60709 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 396526 Chicken
    • Entrez Gene: 280979 Cow
    • Entrez Gene: 403580 Dog
    • Entrez Gene: 60 Human
    • Entrez Gene: 11461 Mouse
    • Entrez Gene: 414396 Pig
    • Entrez Gene: 100009272 Rabbit
    • Entrez Gene: 81822 Rat
    • Entrez Gene: 443052 Sheep
    • Omim: 102630 Human
    • SwissProt: P60706 Chicken
    • SwissProt: P60712 Cow
    • SwissProt: O18840 Dog
    • SwissProt: P60709 Human
    • SwissProt: P60710 Mouse
    • SwissProt: Q6QAQ1 Pig
    • SwissProt: P29751 Rabbit
    • SwissProt: P60711 Rat
    • Unigene: 520640 Human
    • Unigene: 708120 Human
    • Unigene: 727576 Human
    • Unigene: 328431 Mouse
    • Unigene: 391967 Mouse
    • Unigene: 94978 Rat
    see all
  • Alternative names

    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin beta antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • b actin antibody
    • b-actin antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • E430023M04Rik antibody
    • Melanoma X actin antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)
    Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)

    ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence. 

    30 min blocking with TBS-T / 5% BSA
    1 hr incubation with ab6277 (TBS-T / 5% BSA)
    1 minute nuclear counterstaining with DAPI

    Magnification x20

  • Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)
    Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)

    Immunocytochemistry/ Immunofluorescence analysis of chicken fibroblast cells labeling beta Actin with ab6277 at 1/300 dilution. The cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 1/300 Anti-beta Actin antibody [AC-15] (FITC) (ab6277).

     

  • Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)
    Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)

    ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.

    30 min blocking with TBS-T / 5% BSA
    1 hr incubation with ab6277 (TBS-T / 5% BSA)
    1 minute nuclear counterstaining with DAPI

    Magnification x40 no deconvolution.

Protocols

  • Direct IF

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (17)

    Publishing research using ab6277? Please let us know so that we can cite the reference in this datasheet.

    ab6277 has been referenced in 17 publications.

    • Cagnetta R  et al. Noncanonical Modulation of the eIF2 Pathway Controls an Increase in Local Translation during Neural Wiring. Mol Cell 73:474-489.e5 (2019). PubMed: 30595434
    • Réu P  et al. A 61% lighter cell culture dish to reduce plastic waste. PLoS One 14:e0216251 (2019). PubMed: 31039189
    • Cagnetta R  et al. Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome. Neuron 99:29-46.e4 (2018). PubMed: 30008298
    • Olthoff JT  et al. Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle. Nat Commun 9:5104 (2018). PubMed: 30504831
    • Llewellyn KJ  et al. A Fine Balance of Dietary Lipids Improves Pathology of a Murine Model of VCP-Associated Multisystem Proteinopathy. PLoS One 10:e0131995 (2015). WB . PubMed: 26134519
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta Actin antibody [AC-15] (FITC)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Lung)
    Permeabilization
    Yes - 0.1% Triton X
    Specification
    Lung
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. James Xiao

    Verified customer

    Submitted Oct 31 2017

    Western blot abreview for Anti-beta Actin antibody [AC-15] (FITC)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (neuroglioma cells)
    Gel Running Conditions
    Reduced Denaturing (12%Acrylamide SDSPAGE)
    Loading amount
    100 µg
    Treatment
    0.5%triton
    Specification
    neuroglioma cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Read More

    Ms. Samira Acajjaoui

    Verified customer

    Submitted Aug 29 2016

    Question

    Has this antibody been used on formalin fixed, paraffin embedded sections of mouse tissue?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 21 2001

    Answer

    This antibody has not yet been tested for use with on formalin fixed, paraffin embedded sections of mouse tissue.

    Read More

    Abcam Scientific Support

    Answered on Aug 23 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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