FITC Anti-Biotin antibody (ab53469)
Key features and details
- FITC Rabbit polyclonal to Biotin
- Suitable for: ICC/IF
- Reacts with: Species independent
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Overview
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Product name
FITC Anti-Biotin antibody
See all Biotin primary antibodies -
Description
FITC Rabbit polyclonal to Biotin -
Host species
Rabbit -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
d-Biotin conjugated to KLH
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General notes
Fluorescein isothionate; Ab/FITC ratio=approximately 1/20.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Lyophilized:1. Centrifuge the tube at 5000 rpm for 30 seconds to deposit the philosophized powder to the tube bottom; 2. Add 100 uL of distilled water and shake gently for 10 minutes until the powder is completely dissolved; 3. Add 900 uL of 50% glycerol (in distilled water) and shake gently for 5 minutes until all liquid is completely mixed. -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab53469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. |
Target
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Relevance
Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. -
Alternative names
- Vitamin B4 antibody
Images
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Immunocytochemistry/ Immunofluorescence - FITC Anti-Biotin antibody (ab53469)This image is a courtesy of Anonymous Abreview
ab53469 staining Biotin in human ovarian cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized with 0.1% Triton ×100 in PBS and blocking with 5% serum for 30min at 230C was performed. Samples were incubated with primary antibody (1/100) for 1 hour at 23°C.
Protocols
Datasheets and documents
References (2)
ab53469 has been referenced in 2 publications.
- Rubio K et al. Inactivation of nuclear histone deacetylases by EP300 disrupts the MiCEE complex in idiopathic pulmonary fibrosis. Nat Commun 10:2229 (2019). PubMed: 31110176
- Yamamoto-Fukuda T et al. Keratinocyte Growth Factor (KGF) Modulates Epidermal Progenitor Cell Kinetics through Activation of p63 in Middle Ear Cholesteatoma. J Assoc Res Otolaryngol 19:223-241 (2018). PubMed: 29549594
