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    fitc-biotin-antibody-ab6650.pdf

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FITC Anti-Biotin antibody (ab6650)

  • Datasheet
Submit a review Q&A (2)References (2)

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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • FITC Goat polyclonal to Biotin
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Species independent
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

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Conjugation
Product image
FITC Conjugation Kit - Lightning-Link® (ab102884)
Primary
FITC Anti-Human IgG antibody (ab4220)
Primary
Anti-Biotin antibody (ab6643)

View more associated products

Overview

  • Product name

    FITC Anti-Biotin antibody
    See all Biotin primary antibodies
  • Description

    FITC Goat polyclonal to Biotin
  • Host species

    Goat
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule conjugated to KLH

  • General notes

    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.7 moles FITC per mole of Goat IgG

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • Conjugates
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Isotype control

    • FITC Goat IgG - Isotype control (ab37374)

Applications

Our Abpromise guarantee covers the use of ab6650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab37394 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/500 - 1/2500.

Target

  • Relevance

    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • Alternative names

    • Vitamin B4 antibody

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (2)

    Publishing research using ab6650? Please let us know so that we can cite the reference in this datasheet.

    ab6650 has been referenced in 2 publications.

    • Link J  et al. Transient and Partial Nuclear Lamina Disruption Promotes Chromosome Movement in Early Meiotic Prophase. Dev Cell 45:212-225.e7 (2018). PubMed: 29689196
    • Baratta JL  et al. Cellular organization of normal mouse liver: a histological, quantitative immunocytochemical, and fine structural analysis. Histochem Cell Biol 131:713-26 (2009). IHC-FoFr . PubMed: 19255771

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    Hello, Can you tell me how many dyes (fluorescein) are attached per molecule of anti-biotin antibody? Waiting for your reply and thanking you in advance. Cheers.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 18 2002

    Answer

    The molar ratios of dye-to-protein are varied batch to batch. The general acceptance criteria for this product is 2-5:1

    Read More

    Danielle Miller

    Abcam Scientific Support

    Answered on Feb 19 2002

    Question

    Have you used AB6650 (Goat anti-biotin Fluorescein conjugate) for membrane-based detection of Biotinylated nucleic acids on charged nylon membranes (specifically Gene-screen plus)? If so, what membrane blocking reagents have been tested & how did they work? (i.e best, 2nd best etc.)

    Read More

    Abcam community

    Verified customer

    Asked on Aug 24 2001

    Answer

    To our knowledge this antibody has yet to be tested in this application. We would welcome any feedback in this respect from researchers who have tried using this product.

    Read More

    Abcam Scientific Support

    Answered on Aug 29 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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