FITC Anti-c-Fos antibody [H15-S] (ab175647)
Key features and details
- FITC Rabbit monoclonal [H15-S] to c-Fos
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Overview
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Product name
FITC Anti-c-Fos antibody [H15-S]
See all c-Fos primary antibodies -
Description
FITC Rabbit monoclonal [H15-S] to c-Fos -
Host species
Rabbit -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human c-Fos (C terminal).
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Epitope
Antibody detects the linear epitope at the C-terminus of human c-FOS -
Positive control
- Human peripheral blood lymphocytes.
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General notes
The monospecific product was conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.50
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.33% Sodium phosphate
Aqueous buffer -
Concentration information loading...
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Purification notes
This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a propriety technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability. -
Clonality
Monoclonal -
Clone number
H15-S -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 10µl for 106 cells.
(in a 100 µl experimental sample). |
Notes |
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Flow Cyt
Use 10µl for 106 cells. (in a 100 µl experimental sample). |
Target
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Function
Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. -
Sequence similarities
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain. -
Post-translational
modificationsPhosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.
Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2353 Human
- Omim: 164810 Human
- SwissProt: P01100 Human
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Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- C FOS antibody
see all
Images
Datasheets and documents
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Datasheet download
References (1)
ab175647 has been referenced in 1 publication.
- Fast EM et al. External signals regulate continuous transcriptional states in hematopoietic stem cells. Elife 10:N/A (2021). PubMed: 34939923