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  1. Link

    fitc-c-reactive-protein-antibody-ab34659.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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FITC Anti-C Reactive Protein antibody (ab34659)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (3)

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Key features and details

  • FITC Goat polyclonal to C Reactive Protein
  • Suitable for: Immunomicroscopy, Flow Cyt
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

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Overview

  • Product name

    FITC Anti-C Reactive Protein antibody
    See all C Reactive Protein primary antibodies
  • Description

    FITC Goat polyclonal to C Reactive Protein
  • Host species

    Goat
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: Immunomicroscopy, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human C Reactive Protein.
    Database link: P02741

  • Positive control

    • Partially purified human CRP
  • General notes

    2.2moles Fluorescein (Diaminotriazynlamino-fluorescein (DTAF0), 530 daltons) per mole of Goat IgG. Absorption Wavelength: 495 nm Emission Wavelength: 528 nm.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 6.50
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1% BSA, 0.812% Sodium chloride, 0.164% Sodium phosphate

    BSA is IgG and Protease free
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against buffer.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cardiovascular
    • Blood
    • Acute Phase Reactants
    • Immunology
    • Innate Immunity
    • Complement
    • Classical Pathway
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Cardiovascular
    • Heart
    • Apoptosis
    • Metabolism
    • Types of disease
    • Metabolic disorders
    • Neuroscience
    • Processes

Associated products

  • Isotype control

    • FITC Goat IgG - Isotype control (ab37374)
  • Recombinant Protein

    • Recombinant Human C Reactive Protein (ab167710)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab34659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab37394 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab37394 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
  • Tissue specificity

    Found in plasma.
  • Sequence similarities

    Belongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P02741 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1401 Human
    • Omim: 123260 Human
    • SwissProt: P02741 Human
    • Unigene: 709456 Human
    • Unigene: 76452 Human
    • Alternative names

      • Pentraxin 1, short antibody
      • C reactive protein antibody
      • C reactive protein pentraxin related antibody
      • C-reactive protein(1-205) antibody
      • CRP antibody
      • CRP_HUMAN antibody
      • MGC88244 antibody
      • Pentraxin 1 antibody
      • PTX 1 antibody
      • PTX1 antibody
      see all

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab34659? Please let us know so that we can cite the reference in this datasheet.

    ab34659 has been referenced in 3 publications.

    • Fendl B  et al. Extracellular vesicles are associated with C-reactive protein in sepsis. Sci Rep 11:6996 (2021). PubMed: 33772103
    • Kardava L  et al. IgG3 regulates tissue-like memory B cells in HIV-infected individuals. Nat Immunol 19:1001-1012 (2018). PubMed: 30104633
    • Sabharwal V  et al. Capsular switching as a strategy to increase pneumococcal virulence in experimental otitis media model. Microbes Infect N/A:N/A (2013). Flow Cyt ; Human . PubMed: 24361902

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    Dear technical support team:
    This customer raises an enquiry about ab34659 (Anti-C Reactive Protein antibody (FITC) - Azide free), this customer wants to know whether we can offer the fluorescence spectra of this antibody to him or not?
    Could you please help this customer to solve the problem?

    Thanks for your kindly help
    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on May 11 2012

    Answer

    Thank you for your inquiry.

    FITC has an excitation wavelength of 495 and an emission wavelength of 519.

    I attached a fluorochrome guide to this email that can also be found here:

    https://docs.abcam.com/pdf/immunology/fluorochrome_guide.pdf

    I hope this information is helpful and wish you and your customer a good weekend.

    Read More

    Abcam Scientific Support

    Answered on May 11 2012

    Question

    Dear technical support team: This customer raised an enquire about ab34659 (Anti-C Reactive Protein antibody (FITC) - Azide free), he wants to know which position of this antibody is the FITC conjugate with? Could you please help this customer to solve the problem? Thanks for your kindly help Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Dec 05 2011

    Answer

    Thank you for your enquiry. I can confirm that for the most recent lot DTAF (a fluorosein deriviative) was used at a ratio of 2.2 moles per molecule of IgG.  This may vary slightly with each lot.  (FITC is used on most lots) I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Dec 05 2011

    Question

    This customer raises an enquiry about ab34659 (Anti-C Reactive Protein antibody (FITC) - Azide free), he wants to know the diameter (nm) of this antibody, and could you please help this customer to solve the problem? Thanks for your kindly help Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Apr 09 2012

    Answer

    Thank you for your enquiry.

    I have found that the approximate dimensions of an IgG molecule are 14nm x 8.5nm x 4nm.

    I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Apr 09 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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