For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    fitc-c3c-antibody-ab4212.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Innate Immunity Complement Classical Pathway
Share by email

FITC Anti-C3c antibody (ab4212)

  • Datasheet
  • SDS
Reviews (1)Q&A (5)References (6)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - FITC Anti-C3c antibody (ab4212)

    Key features and details

    • FITC Rabbit polyclonal to C3c
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG

    You may also be interested in

    Conjugation
    Product image
    FITC Conjugation Kit - Lightning-Link® (ab102884)
    Primary
    Product image
    Anti-SOX11 antibody [CL0142] (ab154138)
    Primary
    Product image
    FITC Anti-Kappa light chain antibody (ab4208)

    View more associated products

    Overview

    • Product name

      FITC Anti-C3c antibody
      See all C3c primary antibodies
    • Description

      FITC Rabbit polyclonal to C3c
    • Host species

      Rabbit
    • Conjugation

      FITC. Ex: 493nm, Em: 528nm
    • Specificity

      This antibody reacts with human C3c complement and with the C3c part of C3 and C3b.
    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Sheep, Goat, Guinea pig, Cow, Cat, Dog, Pig, Kangaroo, Mink
    • Immunogen

      C3c complement isolated from complement activated human serum.

    • General notes

      Fluorescein isothiocyanate (FITC) isomer 1.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituent: 1% BSA
    • Concentration information loading...
    • Purity

      IgG fraction
    • Purification notes

      Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Innate Immunity
      • Complement
      • Classical Pathway
      • Immunology
      • Innate Immunity
      • Complement
      • Alternative Pathway

    Associated products

    • Isotype control

      • FITC-Rabbit IgG - Isotype Control (ab37406)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab4212 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    Use a concentration of 1 µg/ml.
    Notes
    ICC/IF
    Use a concentration of 1 µg/ml.

    Target

    • Function

      C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
      Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
    • Tissue specificity

      Plasma.
    • Involvement in disease

      Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:613779]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
      Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
      Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
    • Sequence similarities

      Contains 1 anaphylatoxin-like domain.
      Contains 1 NTR domain.
    • Post-translational
      modifications

      C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P01024 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 476728 Dog
      • Entrez Gene: 718 Human
      • Entrez Gene: 12266 Mouse
      • Entrez Gene: 397072 Pig
      • Entrez Gene: 24232 Rat
      • Omim: 120700 Human
      • SwissProt: P01024 Human
      • SwissProt: P01027 Mouse
      • SwissProt: P01025 Pig
      • SwissProt: P01026 Rat
      • Unigene: 529053 Human
      • Unigene: 19131 Mouse
      • Unigene: 11378 Rat
      see all
    • Form

      Cleaved into the following 10 chains: 1) Complement C3 beta chain 2) Complement C3 alpha chain 3) C3a anaphylatoxin 4) Complement C3b alpha' chain 5) Complement C3c alpha' chain fragment 1 6) Complement C3dg fragment 7) Complement C3g fragment 8) Complement C3d fragment 9) Complement C3f fragment 10) Complement C3c alpha' chain fragment 2
    • Alternative names

      • acylation-stimulating protein cleavage product antibody
      • AHUS5 antibody
      • ARMD9 antibody
      • ASP antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
      • C3 antibody
      • c3 complement antibody
      • C3a anaphylatoxin antibody
      • C3a antibody
      • C3b antibody
      • CO3_HUMAN antibody
      • Complement C3 antibody
      • Complement C3c alpha' chain fragment 2 antibody
      • Complement C3c antibody
      • Complement component 3 antibody
      • Complement component C3 antibody
      • Complement component C3a antibody
      • Complement component C3b antibody
      • Complement factor 3 antibody
      • CPAMD1 antibody
      • Prepro C3 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - FITC Anti-C3c antibody (ab4212)
      Immunocytochemistry/ Immunofluorescence - FITC Anti-C3c antibody (ab4212)

      ICC/IF image of ab4212 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4212, 1µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (6)

    Publishing research using ab4212? Please let us know so that we can cite the reference in this datasheet.

    ab4212 has been referenced in 6 publications.

    • Pan T  et al. Circulating Heme Oxygenase-1 and Complement Activation in Transplant-Associated Thrombotic Microangiopathy. Biol Blood Marrow Transplant N/A:N/A (2019). PubMed: 30871975
    • Ispasanie E  et al. Spontaneous point mutations in the capsule synthesis locus leading to structural and functional changes of the capsule in serogroup A meningococcal populations. Virulence 9:1138-1149 (2018). PubMed: 30067453
    • Noone DG  et al. Von Willebrand factor regulates complement on endothelial cells. Kidney Int 90:123-34 (2016). PubMed: 27236750
    • Bahia El Idrissi N  et al. Complement activation at the motor end-plates in amyotrophic lateral sclerosis. J Neuroinflammation 13:72 (2016). PubMed: 27056040
    • Zhang Y  et al. Effect of the glycosyltransferases on the capsular polysaccharide synthesis of Streptococcus suis serotype 2. Microbiol Res 185:45-54 (2016). PubMed: 26946377
    • Kim SJ  et al. Intravitreal human complement factor H in a rat model of laser-induced choroidal neovascularisation. Br J Ophthalmol 97:367-70 (2013). Rat . PubMed: 23258212

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-6 of 6 Abreviews or Q&A

    Question

    Good Morning,

    I have purchased the following product and I was wondering if you would be able to tell me the concentration of antibody:

    Anti-C3c antibody (FITC) (ab4212)

    Thanking you in advance

    Read More

    Abcam community

    Verified customer

    Asked on Aug 20 2012

    Answer

    Thank you for your enquiry and your interest.

    I can confirm that the Ig concentration for this antibody is 1.4g/l.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Aug 20 2012

    Question

    We are interested in buying the following FITC conjugated antibodies on OEM basis. Could you please let us know the price and other technical details?



    · C1q antibody (FITC)

    · C3c antibody (FITC)

    · Human Kappa Chain antibody [SB81a] (FITC)

    · Kappa light chain antibody (FITC)

    · Lambda Light chain antibody (FITC)

    · Mouse monoclonal [JDC-12] Secondary Antibody to Human lambda - light chain (FITC)

    · Mouse monoclonal [A9604D2] Secondary Antibody to Human IgA2 - Fc (FITC)

    Read More

    Abcam community

    Verified customer

    Asked on Jul 30 2012

    Answer

    Thank you for contacting us.

    My colleague has confirmed that we do not currently OEM any of our products for resale. If you are interested in using our products to develop other assays such as kits, my colleagues at mailto:sales@abcam.com can help you with bulk discount information. Unfortunately, we would not be able to provide a quote for reselling the products directly.

    The products fitting your description are:

    ab4223: C1q antibody (FITC)
    ab4212: C3c antibody (FITC)
    ab79118: Human Kappa Chain antibody [SB81a] (FITC)
    ab4208: Kappa light chain antibody (FITC)
    ab9006: Lambda light chain antibody (FITC)
    ab99809: Mouse monoclonal [JDC-12] Secondary Antibody to Human lambda - light chain (FITC)
    ab99799: Mouse monoclonal [A9604D2] Secondary Antibody to Human IgA2 - Fc (FITC)

    The datasheets with the most current information and pricing are linked below. I hope this helps, please let me know if you need any additional information or assistance.

    Read More

    Abcam Scientific Support

    Answered on Jul 30 2012

    Question

    Product wrongly suggested for IHC - on frozen tissue that has been perfusion fixed. I would like to request a different antibody, ab11887

    Read More

    Abcam community

    Verified customer

    Asked on Feb 08 2012

    Answer

    Thank you for contacting Abcam.


    I do apologize for the error in recommending a suitable product for your experiments. I have placed another order, free of charge. I wish you the best of luck with your experiments!

    Included in this email are the instructions for returning your item/s to Abcam. When you return the goods, please ensure that you follow the procedures as below:

    - Return in the original Abcam box with the cooler box and ice pack (or similar) within 7 days from today.
    - Return all documents (or copies of) that arrived with the product (if they are available – should be invoice, delivery note and datasheet)
    - Free shipping: Please return via FEDEX PRIORITY OVERNIGHT SHIPPING and use the Fedex account number provided by Abcam
    - Ship to the following address:

    Attn: Returns ORDER REF: (_____)
    Abcam Inc
    One Kendall Square
    Bldg 200, Suite 341
    Cambridge, MA 02139

    There will be no restock fee on this order due to the error being on our end. The most up-to-date information regarding your order, invoice, and credit note is available online in your Abcam account information. If your order was placed by credit card, your credit card will be issued the refund amount. Please be advised that if your order was placed by Purchase Order, all customers have to submit a credit note with their invoice to their Accounts Payable department to be handled.

    We apologize for this inconvenience you may have been caused by this error. If you have any other questions please do not hesitate to contact us.

    Email: mailto:US.orders@Abcam.com
    Phone: 888-77-ABCAM(22226)
    Fax: 866 739 9884
    Customer Service hours of operation: Monday-Friday 8:30 AM-9:00PM EST

    Read More

    Abcam Scientific Support

    Answered on Feb 08 2012

    Flow Cytometry abreview for Anti-C3c antibody (FITC)

    Good
    Abreviews
    Abreviews
    Application
    Flow Cytometry
    Sample
    Human Cell (ovary)
    Specification
    ovary
    Preparation
    Cell harvesting/tissue preparation method: trypsinisation
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    live cells
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 05 2007

    Question

    BATCH NUMBER 63378 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No staining SAMPLE Rat Kidney Tissue PRIMARY ANTIBODY 100 or 25 fold dilution Reaction was performed at room tempature for 1 hour SECONDARY ANTIBODY Direct observation of primary antibody POSITIVE AND NEGATIVE CONTROLS USED They did not use the possitive control, however they don't know which is best as possitive control. If you possible, please let me know which sample is recomended. They use sample without primary antibody as negative control. ANTIBODY STORAGE CONDITIONS On ice, without freezing FIXATION OF SAMPLE Without fixation ANTIGEN RETRIEVAL No information PERMEABILIZATION STEP No information BLOCKING CONDITIONS Without blocking HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 1 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES Customer hope you to give him some advice on protcol or better possitive control. Please let me know.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 28 2005

    Answer

    I can confirm that the recommended positive control must be human kidney and that the tissue should be fixed in acetone. Unfixed tissue may not be suitable for staining. I hope this information helps, please do not hesitate to contact us if you need further advice,

    Read More

    Abcam Scientific Support

    Answered on Mar 02 2005

    Question

    BATCH NUMBER 63378 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No staining SAMPLE Rat Kidney Tissue PRIMARY ANTIBODY 100 or 25 fold dilution Reaction was performed at room tempature for 1 hour SECONDARY ANTIBODY Direct observation of primary antibody POSITIVE AND NEGATIVE CONTROLS USED They did not use the possitive control, however they don't know which is best as possitive control. If you possible, please let me know which sample is recomended. They use sample without primary antibody as negative control. ANTIBODY STORAGE CONDITIONS On ice, without freezing FIXATION OF SAMPLE Without fixation ANTIGEN RETRIEVAL No information PERMEABILIZATION STEP No information BLOCKING CONDITIONS Without blocking HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 1 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES Customer hope you to give him some advice on protcol or better possitive control. Please let me know.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 28 2005

    Answer

    I'm sorry to hear you are having a problem with ab4212. I would like to suggest the following modifications to your protocol: -make sure the antibody is protected from the light -fix the frozen tissue sections with ice cold acetone or methanol for 5-10min -make sure you dilute both primary and secondary antibodies in PBST (triton 0.3%v/v in PBS) -incubate the antibody overnight at 4C in the dark and make sure the sections are protected from the light at all times. I have contacted the source of ab4212 to find out the recommended positive control, my apologies for the delay. Please let me know if the above suggestions help and do not hesitate to contact us for further advice,

    Read More

    Abcam Scientific Support

    Answered on Feb 28 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.