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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Troponin
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FITC Anti-Cardiac Troponin T antibody [1C11] (ab105439)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (2)

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Key features and details

  • FITC Mouse monoclonal [1C11] to Cardiac Troponin T
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG1

You may also be interested in

Protein
Product image
Recombinant Human Cardiac Troponin T protein (ab86685)

View more associated products

Overview

  • Product name

    FITC Anti-Cardiac Troponin T antibody [1C11]
    See all Cardiac Troponin T primary antibodies
  • Description

    FITC Mouse monoclonal [1C11] to Cardiac Troponin T
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    ab105439 has no cross reaction with skeletal Troponin T.
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Free Human Cardiac Troponin T

  • General notes

    This product was changed from ascites to tissue culture supernatant on 17th October 2017 and product received after this date will be from tissue culture supernatant.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    1C11
  • Myeloma

    Sp2/0
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Troponin
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Troponin
    • Cardiovascular
    • Cardiovascular Markers
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Cardiomyocytes

Associated products

  • Alternative Versions

    • Anti-Cardiac Troponin T antibody [1C11] (ab8295)
  • Isotype control

    • FITC Mouse IgG1 [B11/6] - Isotype Control (ab91356)
  • Recombinant Protein

    • Recombinant Human Cardiac Troponin T protein (ab86685)

Target

  • Function

    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Tissue specificity

    Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • Involvement in disease

    Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • Sequence similarities

    Belongs to the troponin T family.
  • Target information above from: UniProt accession P45379 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7139 Human
    • Omim: 191045 Human
    • SwissProt: P45379 Human
    • Unigene: 533613 Human
    • Alternative names

      • Cardiac muscle troponin T antibody
      • Cardiomyopathy dilated 1D (autosomal dominant) antibody
      • Cardiomyopathy hypertrophic 2 antibody
      • CMD1D antibody
      • CMH2 antibody
      • CMPD2 antibody
      • cTnT antibody
      • LVNC6 antibody
      • MGC3889 antibody
      • OTTHUMP00000033864 antibody
      • OTTHUMP00000033865 antibody
      • OTTHUMP00000033866 antibody
      • OTTHUMP00000033867 antibody
      • OTTHUMP00000033870 antibody
      • OTTHUMP00000218095 antibody
      • RCM3 antibody
      • TNNT 2 antibody
      • TNNT2 antibody
      • TNNT2_HUMAN antibody
      • TnTc antibody
      • Troponin T cardiac muscle antibody
      • Troponin T type 2 (cardiac) antibody
      • Troponin T type 2 cardiac antibody
      • Troponin T, cardiac muscle antibody
      • Troponin T2 cardiac antibody
      see all

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab105439? Please let us know so that we can cite the reference in this datasheet.

    ab105439 has been referenced in 2 publications.

    • Ferrer CM  et al. An inactivating mutation in the histone deacetylase SIRT6 causes human perinatal lethality. Genes Dev 32:373-388 (2018). PubMed: 29555651
    • Wang X  et al. Selenium Augments microRNA Directed Reprogramming of Fibroblasts to Cardiomyocytes via Nanog. Sci Rep 6:23017 (2016). Flow Cyt . PubMed: 26975336

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    One our customers wants to purchase antibodies to test in sELISA and human samples. They want to detect cardiac troponins T and I. 1) anti-cardiac troponin T : ab45932 to coat on surface, then the protein ab47005 and then the FITC conjugated antibody ab105439 for detection. 2) anti-cardiac troponin I : ab27972, then Cardiac Troponin I protein ab50803 and ab56357 that will be conjugated with FITC using the Easylink kit. Would it work for their sELISA experiments?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 15 2011

    Answer

    Thank you for contacting us. I would recommend to use : 1) For the detection of Cardiac Troponin T : - ab45932 as capture antibody (directed against amino acids 64-82) www.abcam.com/ab45932 , - ab9937 as protein target, www.abcam.com/ab9937 , (not ab47005 which is the immunogen peptide used to raise ab45932), - ab105439 as detection antibody (directed against amino acids 64-82), www.abcam.com/ab105439 . 2) For the detection of Cardiac Troponin I : - ab115704 as capture antibody, www.abcam.com/ab115704 , - ab50803 as protein target, www.abcam.com/ab50803 , - ab19615 as detection antibody (can be conjugated with FITC using the EasyLink conjugation kit), www.abcam.com/ab19615 . I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Sep 15 2011

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