FITC Anti-CCR9 antibody [CW-1.2] (ab95670)
![Flow Cytometry - FITC Anti-CCR9 antibody [CW-1.2] (ab95670)](https://www.abcam.com/ps/products/95/ab95670/Images/ab95670-109932-anti-ccr9-antibody-cw-12-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-CCR9 antibody [CW-1.2] (ab95670)")
Key features and details
- FITC Mouse monoclonal [CW-1.2] to CCR9
- Suitable for: Flow Cyt
- Reacts with: Mouse, Rat, Monkey
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Overview
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Product name
FITC Anti-CCR9 antibody [CW-1.2]
See all CCR9 primary antibodies -
Description
FITC Mouse monoclonal [CW-1.2] to CCR9 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Monkey -
Immunogen
WT Mouse thymocytes (Eur J Immunol. 2006 Jan;36(1):73-81)
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Positive control
- Mouse thymocytes and splenocytes (BALB/c splenocytes)
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
CW-1.2 -
Isotype
IgG2a -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab95670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.125-0.25µg for 105-8 cells. A final volume of 100 µL is recommended for staining the cell sample.
ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. -
Tissue specificity
Highly expressed in the thymus and low in lymph nodes and spleen. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12769 Mouse
- Entrez Gene: 282832 Rat
- SwissProt: Q9WUT7 Mouse
- Unigene: 440604 Mouse
- Unigene: 442383 Mouse
- Unigene: 91140 Rat
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Alternative names
- C C chemokine receptor D6 antibody
- C C chemokine receptor type 9 antibody
- C C CKR 9 antibody
see all
Images
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Flow Cytometry - FITC Anti-CCR9 antibody [CW-1.2] (ab95670)Flow cytometry staining of BALB/c splenocytes with 0.125 µg of Mouse IgG2a ? Isotype Control FITC (open histogram) or 0.125 µg of ab95670 (filled histogram). Cells in the lymphocyte gate were used for analysis and gated on either CD4+ (left) or CD8+ (right) events.
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![Flow Cytometry - FITC Anti-CCR9 antibody [CW-1.2] (ab95670)](https://www.abcam.com/ps/products/95/ab95670/Images/ab95670-109931-anti-ccr9-antibody-cw-12-fitc-flow-cytometry.jpg)
Flow Cytometry - FITC Anti-CCR9 antibody [CW-1.2] (ab95670)This image is courtesy of an anonymous Abreviewab95670 staining CCR9 in Rat splenocytes by Flow Cytometry. Rat spleen was homogenized and red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.
Datasheets and documents
References (1)
ab95670 has been referenced in 1 publication.
- Wurbel MA et al. Complex regulation of CCR9 at multiple discrete stages of T cell development. Eur J Immunol 36:73-81 (2006). PubMed: 16342233
