FITC Anti-CD105 antibody [MEM-229] (ab53318)
![Immunohistochemistry (Frozen sections) - FITC Anti-CD105 antibody [MEM-229] (ab53318)](https://www.abcam.com/ps/products/53/ab53318/Images/ab53318-66100-anti-cd105-antibody-mem-229-fitc-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - FITC Anti-CD105 antibody [MEM-229] (ab53318)")
Key features and details
- FITC Mouse monoclonal [MEM-229] to CD105
- Suitable for: IHC-Fr
- Reacts with: Pig
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
FITC Anti-CD105 antibody [MEM-229]
See all CD105 primary antibodies -
Description
FITC Mouse monoclonal [MEM-229] to CD105 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
The antibody MEM-229 recognizes an extracellular epitope of CD105 (Endoglin), a 90 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B
lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy. -
Tested applications
Suitable for: IHC-Frmore details -
Species reactivity
Reacts with: Pig -
Immunogen
Recombinant fragment corresponding to Human CD105.
Database link: P17813 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Purified by size-exclusion chromatography. -
Clonality
Monoclonal -
Clone number
MEM-229 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
1/200. Fix with acetone.
|
| Notes |
|---|
|
IHC-Fr
1/200. Fix with acetone. |
Target
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Function
Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. -
Tissue specificity
Endoglin is restricted to endothelial cells in all tissues except bone marrow. -
Involvement in disease
Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- AI528660 antibody
- AI662476 antibody
- CD 105 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - FITC Anti-CD105 antibody [MEM-229] (ab53318)ab53318 staining CD105 in infarcted porcine heart by Immunohistochemistry (Frozen sections). Cell nuclei were counterstained blue with DAPI.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab53318 has been referenced in 20 publications.
- Oh J et al. Mesenchymal stem cells genetically engineered to express platelet-derived growth factor and heme oxygenase-1 ameliorate osteoarthritis in a canine model. J Orthop Surg Res 16:43 (2021). PubMed: 33430899
- Chen Y et al. MSC-Secreted Exosomal H19 Promotes Trophoblast Cell Invasion and Migration by Downregulating let-7b and Upregulating FOXO1. Mol Ther Nucleic Acids 19:1237-1249 (2020). PubMed: 32069774
- Meng Q & Qiu B Exosomal MicroRNA-320a Derived From Mesenchymal Stem Cells Regulates Rheumatoid Arthritis Fibroblast-Like Synoviocyte Activation by Suppressing CXCL9 Expression. Front Physiol 11:441 (2020). PubMed: 32528301
- Cao G et al. Human Adipose-Derived Mesenchymal Stem Cells-Derived Exosomal microRNA-19b Promotes the Healing of Skin Wounds Through Modulation of the CCL1/TGF-ß Signaling Axis. Clin Cosmet Investig Dermatol 13:957-971 (2020). PubMed: 33364805
- Dothel G et al. Ex vivo effect of vascular wall stromal cells secretome on enteric ganglia. World J Gastroenterol 25:4892-4903 (2019). PubMed: 31543681