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  1. Link

    fitc-cd11b-antibody-m170-ab24874.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Alpha
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FITC Anti-CD11b antibody [M1/70] (ab24874)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (9)

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Flow Cytometry - FITC Anti-CD11b antibody [M1/70] (ab24874)
  • Flow Cytometry - FITC Anti-CD11b antibody [M1/70] (ab24874)

Key features and details

  • FITC Rat monoclonal [M1/70] to CD11b
  • Suitable for: Flow Cyt
  • Reacts with: Mouse, Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG2b

You may also be interested in

Protein
Recombinant Human CD11b protein (ab126010)

View more associated products

Overview

  • Product name

    FITC Anti-CD11b antibody [M1/70]
    See all CD11b primary antibodies
  • Description

    FITC Rat monoclonal [M1/70] to CD11b
  • Host species

    Rat
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    Alpha M subunit (Mr 170 kDa) of the CD11b/CD18 heterodimer.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit
  • Immunogen

    Tissue/ cell preparation (Mouse): C57BL/10 mouse splenic T cells and concanavaline A-activated C57BL/10 splenocytes.

  • Positive control

    • BALB/c spleen cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze. Store undiluted.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    M1/70
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Microglia marker
    • Stem Cells
    • Mesenchymal Stem Cells
    • Negative Markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Murine Lineage Negative
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-CD11b antibody [M1/70] (ab197701)
    • Alexa Fluor® 647 Anti-CD11b antibody [M1/70] (ab197702)
    • Alexa Fluor® 405 Anti-CD11b antibody [M1/70] (ab197703)
    • PerCP/Cy5.5® Anti-CD11b antibody [M1/70] (ab210299)
    • PE/Cy7® Anti-CD11b antibody [M1/70] (ab218786)
    • PerCP Anti-CD11b antibody [M1/70] (ab237025)
    • violetFluor™ 450 Anti-CD11b antibody [M1/70] (ab242266)
    • PE Anti-CD11b antibody [M1/70] (ab25175)
    • redFluor™ 710 Anti-CD11b antibody [M1/70] (ab253091)
    • APC Anti-CD11b antibody [M1/70] (ab25482)
    • PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
    • APC/Cy7® Anti-CD11b antibody [M1/70] (ab79096)
    • Anti-CD11b antibody [M1/70] (ab8878)
  • Isotype control

    • FITC Rat IgG2b, kappa monoclonal [eB149/10H5] - Isotype control (ab136125)
  • Recombinant Protein

    • Recombinant Human CD11b protein (ab126010)

Applications

Our Abpromise guarantee covers the use of ab24874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab136125 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • Tissue specificity

    Predominantly expressed in monocytes and granulocytes.
  • Involvement in disease

    Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P11215 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3684 Human
    • Entrez Gene: 16409 Mouse
    • Entrez Gene: 100351865 Rabbit
    • Omim: 120980 Human
    • SwissProt: P11215 Human
    • SwissProt: P05555 Mouse
    • Unigene: 172631 Human
    • Unigene: 262106 Mouse
    • Alternative names

      • antigen CD11b (p170) antibody
      • Antigen CD11b p170 antibody
      • CD11 antigen like family member B antibody
      • CD11 antigen-like family member B antibody
      • CD11b antibody
      • CD11b/CD18 antibody
      • CD49d antibody
      • Cell surface glycoprotein MAC-1 subunit alpha antibody
      • Complement component 3 receptor 3 subunit antibody
      • Complement Component Receptor 3 Alpha antibody
      • Complement receptor type 3 antibody
      • Complement receptor type 3, alpha subunit antibody
      • CR 3 alpha chain (CR3A) antibody
      • CR 3 alpha chain antibody
      • CR-3 alpha chain antibody
      • CR3 antibody
      • CR3A antibody
      • F730045J24Rik antibody
      • Integrin Alpha M antibody
      • Integrin alpha M chain antibody
      • Integrin alpha-M antibody
      • Integrin beta 2 alpha subunit antibody
      • Integrin subunit alpha M antibody
      • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
      • ITAM_HUMAN antibody
      • ITGAM antibody
      • Leukocyte adhesion receptor MO1 antibody
      • Ly-40 antibody
      • MAC 1 antibody
      • Mac-1a antibody
      • MAC1 antibody
      • Mac1, alpha subunit antibody
      • MAC1A antibody
      • Macrophage antigen alpha polypeptide antibody
      • MGC117044 antibody
      • Mo1 antibody
      • Mo1, alpha subunit antibody
      • MO1A antibody
      • Neutrophil adherence receptor alpha M subunit antibody
      • Neutrophil adherence receptor antibody
      • SLEB6 antibody
      see all

    Images

    • Flow Cytometry - FITC Anti-CD11b antibody [M1/70] (ab24874)
      Flow Cytometry - FITC Anti-CD11b antibody [M1/70] (ab24874)

      Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling CD11b with ab24874 at 0.5µg (solid line), Rat IgG2b (FITC)isotype control (dashed line) at 0.5µg concentration.

    • Flow Cytometry - FITC Anti-CD11b antibody [M1/70] (ab24874)
      Flow Cytometry - FITC Anti-CD11b antibody [M1/70] (ab24874)

      ab24874 at 1 µg/106 cells staining BALB/c splenocytes. Cells were then double stained with Rat monoclonal [RB6-8C5] to Ly6g (Phycoerythrin) (ab24884).  Lymphocytes were gated and analyzed by flow cytometry.

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (9)

    Publishing research using ab24874? Please let us know so that we can cite the reference in this datasheet.

    ab24874 has been referenced in 9 publications.

    • Dey R & Bishayi B Dexamethasone along with ciprofloxacin modulates S. aureus induced microglial inflammation via glucocorticoid (GC)-GC receptor-mediated pathway. Microb Pathog 145:104227 (2020). PubMed: 32360190
    • Kawano T  et al. Temporal and spatial profile of polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) in ischemic stroke in mice. PLoS One 14:e0215482 (2019). PubMed: 31048856
    • Benedicto A  et al. CXCR4 receptor blockage reduces the contribution of tumor and stromal cells to the metastatic growth in the liver. Oncol Rep 39:2022-2030 (2018). PubMed: 29436696
    • Karlen SJ  et al. Monocyte infiltration rather than microglia proliferation dominates the early immune response to rapid photoreceptor degeneration. J Neuroinflammation 15:344 (2018). PubMed: 30553275
    • Ruiz de Azua I  et al. Adipocyte cannabinoid receptor CB1 regulates energy homeostasis and alternatively activated macrophages. J Clin Invest 127:4148-4162 (2017). PubMed: 29035280
    • Bai YQ  et al. Effects of the eukaryotic initiation factor 6 gene on expression levels of inflammatory mediators in M2 macrophages during scar repair. Mol Med Rep 14:819-24 (2016). PubMed: 27220600
    • Liu T  et al. Gr-1+CD11b+ cells facilitate Lewis lung cancer recurrence by enhancing neovasculature after local irradiation. Sci Rep 4:4833 (2014). IHC-Fr ; Mouse . PubMed: 24776637
    • Gil M  et al. Surfactant Protein A Modulates Cell Surface Expression of CR3 on Alveolar Macrophages and Enhances CR3-mediated Phagocytosis. J Biol Chem 284:7495-504 (2009). Flow Cyt . PubMed: 19155216
    • Flotte TJ  et al. Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets. Am J Pathol 111:112-24 (1983). PubMed: 6340516

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD11b antibody [M1/70] (FITC)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (mouse hepatocytes)
    Permeabilization
    Yes - 0.1% Triton X-100 in TBS
    Specification
    mouse hepatocytes
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 10 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-CD11b antibody [M1/70] (FITC)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (liver)
    Specification
    liver
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
    Fixative
    OCT
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 16 2015

    Flow Cytometry abreview for Anti-CD11b antibody [M1/70] (FITC)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (splenocytes)
    Specification
    splenocytes
    Preparation
    Cell harvesting/tissue preparation method: cell suspension
    Sample buffer: PBS
    Permeabilization
    No
    Gating Strategy
    Live cells
    Read More

    Abcam user community

    Verified customer

    Submitted May 19 2010

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