FITC Anti-CD11b antibody [M1/70] (ab24874)
![Flow Cytometry - FITC Anti-CD11b antibody [M1/70] (ab24874)](https://www.abcam.com/ps/products/24/ab24874/Images/ab24874-456671-fitc-anti-cd11b-antibody-m1-70-flow-cytometry-bone-marrow-c57bl-6-mouse.jpg "Flow Cytometry - FITC Anti-CD11b antibody [M1/70] (ab24874)")
Key features and details
- FITC Rat monoclonal [M1/70] to CD11b
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
FITC Anti-CD11b antibody [M1/70]
See all CD11b primary antibodies -
Description
FITC Rat monoclonal [M1/70] to CD11b -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
Alpha M subunit (Mr 170 kDa) of the CD11b/CD18 heterodimer. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Human
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Immunogen
Tissue/ cell preparation (Mouse): C57BL/10 mouse splenic T cells and concanavaline A-activated C57BL/10 splenocytes.
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Positive control
- Flow Cyt: Mouse C57BL/6 bone marrow cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
M1/70 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-CD11b antibody [M1/70] (ab197701)
- Alexa Fluor® 647 Anti-CD11b antibody [M1/70] (ab197702)
- PerCP/Cy5.5® Anti-CD11b antibody [M1/70] (ab210299)
- PE/Cy7® Anti-CD11b antibody [M1/70] (ab218786)
- violetFluor™ 450 Anti-CD11b antibody [M1/70] (ab242266)
- PE Anti-CD11b antibody [M1/70] (ab25175)
- redFluor™ 710 Anti-CD11b antibody [M1/70] (ab253091)
- APC Anti-CD11b antibody [M1/70] (ab25482)
- PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
- APC/Cy7® Anti-CD11b antibody [M1/70] (ab79096)
- Anti-CD11b antibody [M1/70] (ab8878)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | (1) |
Use at an assay dependent concentration.
ab136125 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. ab136125 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. -
Tissue specificity
Predominantly expressed in monocytes and granulocytes. -
Involvement in disease
Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3684 Human
- Entrez Gene: 16409 Mouse
- Omim: 120980 Human
- SwissProt: P11215 Human
- SwissProt: P05555 Mouse
- Unigene: 172631 Human
- Unigene: 262106 Mouse
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Alternative names
- antigen CD11b (p170) antibody
- Antigen CD11b p170 antibody
- CD11 antigen like family member B antibody
see all
Images
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Flow Cytometry - FITC Anti-CD11b antibody [M1/70] (ab24874)
Flow Cytometry analysis of Mouse C57BL/6 bone marrow cells labeling CD11b with ab24874 at 0.5µg (solid line), Rat IgG2b (FITC) isotype control (dashed line) at 0.5µg concentration.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab24874 has been referenced in 12 publications.
- Meng Q et al. Human bone marrow mesenchymal stem cell-derived extracellular vesicles impede the progression of cervical cancer via the miR-144-3p/CEP55 pathway. J Cell Mol Med 25:1867-1883 (2021). PubMed: 33417281
- Wu S et al. The long noncoding RNA LINC01140/miR-140-5p/FGF9 axis modulates bladder cancer cell aggressiveness and macrophage M2 polarization. Aging (Albany NY) 12:25845-25864 (2020). PubMed: 33234721
- Coskun FS et al. sncRNA-1 Is a Small Noncoding RNA Produced by Mycobacterium tuberculosis in Infected Cells That Positively Regulates Genes Coupled to Oleic Acid Biosynthesis. Front Microbiol 11:1631 (2020). PubMed: 32849337
- Dey R & Bishayi B Dexamethasone along with ciprofloxacin modulates S. aureus induced microglial inflammation via glucocorticoid (GC)-GC receptor-mediated pathway. Microb Pathog 145:104227 (2020). PubMed: 32360190
- Kawano T et al. Temporal and spatial profile of polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) in ischemic stroke in mice. PLoS One 14:e0215482 (2019). PubMed: 31048856