FITC Anti-CD11c antibody [BU15] (ab22540)
Key features and details
- FITC Mouse monoclonal [BU15] to CD11c
- Suitable for: Flow Cyt
- Reacts with: Dog, Human, Cynomolgus monkey
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-CD11c antibody [BU15]
See all CD11c primary antibodies -
Description
FITC Mouse monoclonal [BU15] to CD11c -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
This antibody recognises the integrin alphax subunit, a 150kD glycoprotein expressed by macrophages, monocytes, granulocytes and NK cells. The antigen is also expressed by hairy cell leukaemia cells. Ligands for CD11c are ICAM 1, iC3b, and fibrinogen. The molecule has a role in mediating adherence of granulocytes and monocytes to endothelium and fibrinogen. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Dog, Human, Cynomolgus monkey -
Immunogen
The details of the immunogen for this antibody are not available.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
BU15 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22540 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/1 - 1/10.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. Use 10µl of working dilution for 106 cells. |
| Notes |
|---|
|
Flow Cyt
1/1 - 1/10. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. Use 10µl of working dilution for 106 cells. |
Target
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Function
Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. -
Tissue specificity
Predominantly expressed in monocytes and granulocytes. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3687 Human
- Omim: 151510 Human
- SwissProt: P20702 Human
- Unigene: 248472 Human
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Alternative names
- 95 alpha chain antibody
- 95 antibody
- CD 11c antibody
see all
Datasheets and documents
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Datasheet download
References (4)
ab22540 has been referenced in 4 publications.
- Hopfensperger K et al. Convergent Evolution of HLA-C Downmodulation in HIV-1 and HIV-2. mBio 11:N/A (2020). PubMed: 32665270
- Langer S et al. HIV-1 Vpu is a potent transcriptional suppressor of NF-?B-elicited antiviral immune responses. Elife 8:N/A (2019). PubMed: 30717826
- Braun E et al. Guanylate-Binding Proteins 2 and 5 Exert Broad Antiviral Activity by Inhibiting Furin-Mediated Processing of Viral Envelope Proteins. Cell Rep 27:2092-2104.e10 (2019). PubMed: 31091448
- Rezzonico R et al. Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta production in primary human monocytes through a pathway dependent on nuclear factor-kappaB. Blood 97:2932-40 (2001). PubMed: 11342414
