FITC Anti-CD14 antibody [MEM-15] (ab28061)
![Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (ab28061)](https://www.abcam.com/ps/products/28/ab28061/Images/ab28061-284947-anti-cd14-antibody-mem-15-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (ab28061)")
Key features and details
- FITC Mouse monoclonal [MEM-15] to CD14
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-CD14 antibody [MEM-15]
See all CD14 primary antibodies -
Description
FITC Mouse monoclonal [MEM-15] to CD14 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
Ab28061 recognises CD14 expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. Ab28061 also recognises soluble forms of CD14 found in serum and in the urine of some nephrotic patients. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified): A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
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General notes
The antibody is free of unconjugated FITC.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituent: 0.2% BSA
High grade protease free BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MEM-15 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PerCP/Cy5.5® Anti-CD14 antibody [MEM-15], prediluted (ab157312)
- APC/Cy7® Anti-CD14 antibody [MEM-15], prediluted (ab194805)
- PE/Cy5® Anti-CD14 antibody [MEM-15] (ab232910)
- PE/Cy7® Anti-CD14 antibody [MEM-15] (ab234214)
- Alexa Fluor® 700 Anti-CD14 antibody [MEM-15] (ab269326)
- PerCP Anti-CD14 antibody [MEM-15] (ab269327)
- Biotin Anti-CD14 antibody [MEM-15] (ab28118)
- APC Anti-CD14 antibody [MEM-15] (ab60901)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab28061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 20µl for 106 cells. or 100 µl of whole blood. |
Target
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Function
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. -
Tissue specificity
Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. -
Sequence similarities
Contains 11 LRR (leucine-rich) repeats. -
Post-translational
modificationsN- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 929 Human
- Omim: 158120 Human
- SwissProt: P08571 Human
- Unigene: 163867 Human
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Alternative names
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
see all
Images
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Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (ab28061)
Flow cytometry analysis of human peripheral blood cells labelling CD14 with ab28061.
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![Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (ab28061)](https://www.abcam.com/ps/products/28/ab28061/Images/ab28061-38961-anti-cd14-antibody-mem-15-fitc-flow-cytometry.jpg)
Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (ab28061)This image is courtesy of an anonymous Abreviewab28061 staining CD14 in peripheral blood mononuclear cells by flow cytometry. The fibroblasts were prepared using 10 uL FITC antiCD14 to 50 uL (~100,000) cells in PBS/5%FCS and incubated on ice for 45 min. Cells were gated on viable cells.
Protocols
References (5)
ab28061 has been referenced in 5 publications.
- Gonda K et al. Myeloid-derived suppressor cells are increased and correlated with type 2 immune responses, malnutrition, inflammation, and poor prognosis in patients with breast cancer. Oncol Lett 14:1766-1774 (2017). PubMed: 28789407
- Shi Q et al. Differentiation of human umbilical cord Wharton's jelly-derived mesenchymal stem cells into endometrial cells. Stem Cell Res Ther 8:246 (2017). PubMed: 29096715
- Jensen J et al. Functionalization of polycaprolactone scaffolds with hyaluronic acid and ß-TCP facilitates migration and osteogenic differentiation of human dental pulp stem cells in vitro. Tissue Eng Part A 21:729-39 (2015). PubMed: 25252795
- Steensma MR et al. Targeting the giant cell tumor stromal cell: functional characterization and a novel therapeutic strategy. PLoS One 8:e69101 (2013). Flow Cyt ; Human . PubMed: 23922683
- Sing A et al. Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression. J Exp Med 196:1017-24 (2002). PubMed: 12391013
