For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    fitc-cd14-antibody-mem-15-ab28061.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Cell Type Markers CD Myeloid Cells
Share by email

FITC Anti-CD14 antibody [MEM-15] (ab28061)

  • Datasheet
  • SDS
Reviews (4)Q&A (1)References (5)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (ab28061)
  • Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (ab28061)

Key features and details

  • FITC Mouse monoclonal [MEM-15] to CD14
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG1

You may also be interested in

Protein
Product image
Recombinant Mouse CD14 protein (ab207103)

View more associated products

Overview

  • Product name

    FITC Anti-CD14 antibody [MEM-15]
    See all CD14 primary antibodies
  • Description

    FITC Mouse monoclonal [MEM-15] to CD14
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    Ab28061 recognises CD14 expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. Ab28061 also recognises soluble forms of CD14 found in serum and in the urine of some nephrotic patients.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified): A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.

  • General notes

    The antibody is free of unconjugated FITC.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituent: 0.2% BSA

    High grade protease free BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    MEM-15
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Myeloid Cells
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Stem Cells
    • Mesenchymal Stem Cells
    • Negative Markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Immunology
    • Innate Immunity
    • TLR Signaling
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • CD markers ELISA kits

Associated products

  • Alternative Versions

    • PerCP/Cy5.5® Anti-CD14 antibody [MEM-15], prediluted (ab157312)
    • APC/Cy7® Anti-CD14 antibody [MEM-15], prediluted (ab194805)
    • PE/Cy5® Anti-CD14 antibody [MEM-15] (ab232910)
    • PE/Cy7® Anti-CD14 antibody [MEM-15] (ab234214)
    • Alexa Fluor® 700 Anti-CD14 antibody [MEM-15] (ab269326)
    • PerCP Anti-CD14 antibody [MEM-15] (ab269327)
    • Biotin Anti-CD14 antibody [MEM-15] (ab28118)
    • APC Anti-CD14 antibody [MEM-15] (ab60901)
  • Isotype control

    • FITC Mouse IgG1 [B11/6] - Isotype Control (ab91356)
  • Recombinant Protein

    • Recombinant Mouse CD14 protein (ab207103)

Applications

Our Abpromise guarantee covers the use of ab28061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 20µl for 106 cells.

or 100 µl of whole blood.

Target

  • Function

    Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • Tissue specificity

    Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • Sequence similarities

    Contains 11 LRR (leucine-rich) repeats.
  • Post-translational
    modifications

    N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P08571 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 929 Human
    • Omim: 158120 Human
    • SwissProt: P08571 Human
    • Unigene: 163867 Human
    • Alternative names

      • CD 14 antibody
      • CD_antigen=CD14 antibody
      • CD14 antibody
      • CD14 antigen antibody
      • CD14 molecule antibody
      • CD14_HUMAN antibody
      • LPS-R antibody
      • Mo2 antibody
      • Monocyte differentiation antigen CD14 antibody
      • Monocyte differentiation antigen CD14 urinary form antibody
      • Monocyte differentiation antigen CD14, membrane-bound form antibody
      • Myeloid cell specific leucine rich glycoprotein antibody
      • Myeloid cell-specific leucine-rich glycoprotein antibody
      see all

    Images

    • Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (ab28061)
      Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (ab28061)

      Flow cytometry analysis of human peripheral blood cells labelling CD14 with ab28061.

    • Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (ab28061)
      Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (ab28061)This image is courtesy of an anonymous Abreview
      ab28061 staining CD14 in peripheral blood mononuclear cells by flow cytometry. The fibroblasts were prepared using 10 uL FITC antiCD14 to 50 uL (~100,000) cells in PBS/5%FCS and incubated on ice for 45 min. Cells were gated on viable cells.

      See Abreview

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (5)

    Publishing research using ab28061? Please let us know so that we can cite the reference in this datasheet.

    ab28061 has been referenced in 5 publications.

    • Gonda K  et al. Myeloid-derived suppressor cells are increased and correlated with type 2 immune responses, malnutrition, inflammation, and poor prognosis in patients with breast cancer. Oncol Lett 14:1766-1774 (2017). PubMed: 28789407
    • Shi Q  et al. Differentiation of human umbilical cord Wharton's jelly-derived mesenchymal stem cells into endometrial cells. Stem Cell Res Ther 8:246 (2017). PubMed: 29096715
    • Jensen J  et al. Functionalization of polycaprolactone scaffolds with hyaluronic acid and ß-TCP facilitates migration and osteogenic differentiation of human dental pulp stem cells in vitro. Tissue Eng Part A 21:729-39 (2015). PubMed: 25252795
    • Steensma MR  et al. Targeting the giant cell tumor stromal cell: functional characterization and a novel therapeutic strategy. PLoS One 8:e69101 (2013). Flow Cyt ; Human . PubMed: 23922683
    • Sing A  et al. Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression. J Exp Med 196:1017-24 (2002). PubMed: 12391013

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD14 antibody [MEM-15] (FITC)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Bone marrow)
    Permeabilization
    No
    Specification
    Bone marrow
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 21 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-CD14 antibody [MEM-15] (FITC)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Acetone
    Read More

    Herr Dr. Christian Ostalecki

    Verified customer

    Submitted May 29 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD14 antibody [MEM-15] (FITC)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 2% · Temperature: 25°C
    Sample
    Human Cell (Peripheral Blood Monocyte)
    Specification
    Peripheral Blood Monocyte
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 15 2014

    Question

    Hello, I ordered and received the CD14-antibody (cat#ab28061), but it was unfortunately stored at -20ºC for a couple of days (although not frozen). I wanted to know whether this would be a problem or if you thought the antibody should be fine. Thanks very much for getting back to me on this. Best,

    Read More

    Abcam community

    Verified customer

    Asked on Dec 12 2011

    Answer

    Thank you for your inquiry. We do not recommend to freeze this antibody and to especially avoid freeze/thaw cycles. But I would like to recommend to run a test experiment. Antibodies in general are very stable and as long as the conjugate did not get damaged by the freezing, the antibody might work just fine. I am especially optimistic since there were no freeze/thaw cycles involved. Unfortunately, we can only guarantee our products to work if they were stored as instructed and cannot make any assumptions on the quality of this antibody after freezing since this was not tested by us experimentally. I wish you good luck with the experiment and hope that the antibody will work well for you.

    Read More

    Abcam Scientific Support

    Answered on Dec 12 2011

    Flow Cytometry abreview for Anti-CD14 antibody [MEM-15] (FITC)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Peripheral Blood Mononuclear Cell)
    Specification
    Peripheral Blood Mononuclear Cell
    Fixation
    none
    Permeabilization
    No
    Gating Strategy
    monocytes
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 20 2009

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.