FITC Anti-CD14 antibody [MEM-18] (ab239250)

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Flow Cytometry - FITC Anti-CD14 antibody [MEM-18] (ab239250)

    Key features and details

    • FITC Mouse monoclonal [MEM-18] to CD14
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    Overview

    • Product name

      FITC Anti-CD14 antibody [MEM-18]
      See all CD14 primary antibodies

    • Description

      FITC Mouse monoclonal [MEM-18] to CD14

    • Host species

      Mouse

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Non human primates

    • Immunogen

      Tissue, cells or virus corresponding to Human CD14. (A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria).

    • Positive control

      • Flow Cyt: Human peripheral blood leukocytes.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab239250 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 20µl for 106 cells.

    (or 100 μl of whole blood).

    Target

    • Function

      Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

    • Tissue specificity

      Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

    • Sequence similarities

      Contains 11 LRR (leucine-rich) repeats.

    • Post-translational
      modifications

      N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 14 antibody
        • CD_antigen=CD14 antibody
        • CD14 antibody
        • CD14 antigen antibody
        • CD14 molecule antibody
        • CD14_HUMAN antibody
        • LPS-R antibody
        • Mo2 antibody
        • Monocyte differentiation antigen CD14 antibody
        • Monocyte differentiation antigen CD14 urinary form antibody
        • Monocyte differentiation antigen CD14, membrane-bound form antibody
        • Myeloid cell specific leucine rich glycoprotein antibody
        • Myeloid cell-specific leucine-rich glycoprotein antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-CD14 antibody [MEM-18] (ab239250)
        Flow Cytometry - FITC Anti-CD14 antibody [MEM-18] (ab239250)

        Flow cytometric analysis of human peripheral blood leukocytes labeling CD14 with ab239250. Surface staining. Gated on leukocytes.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab239250? Please let us know so that we can cite the reference in this datasheet.

      ab239250 has not yet been referenced specifically in any publications.

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