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Immunology Cell Type Markers CD Endothelial Cells
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FITC Anti-CD146 antibody [P1H12] (ab78451)

  • Datasheet
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Flow Cytometry - FITC Anti-CD146 antibody [P1H12] (ab78451)

    Key features and details

    • FITC Mouse monoclonal [P1H12] to CD146
    • Suitable for: Flow Cyt
    • Reacts with: Mouse, Dog, Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    You may also be interested in

    Protein
    Product image
    Recombinant Human CD146 protein (ab114180)
    Primary
    Anti-STRO1 antibody [STRO-1] (ab214086)

    View more associated products

    Overview

    • Product name

      FITC Anti-CD146 antibody [P1H12]
      See all CD146 primary antibodies
    • Description

      FITC Mouse monoclonal [P1H12] to CD146
    • Host species

      Mouse
    • Conjugation

      FITC. Ex: 493nm, Em: 528nm
    • Specificity

      ab78451 reacts specifically with CD146. In blood and marrow ab78451 reacts only with endothelial cells, and has been used to detect circulating endothelial cells in human, dogs, rabbits and mouse samples. ab78451 positively stains normal, primary HUVEC and MVEC cultures and the endothelial cells of all vessels in normal frozen sections of human skin, intestine, ovary tonsil, lymph node, lung, and kidney. It does not stain carcinoma cell lines HT-29 and COLO205, and M21, the T cell lines Jurkat and HuT78, fibroblasts, HL-60 or CHO cells, EBV-transformed B cell lines, normal monocytes, granulocytes, red cells, platelets, T cells or B cells from marrow or peripheral blood; nor does it detect marrow megakaryocytes or the megakaryoblast line HU3. The peripheral blood cells that do stain with ab78451 are also positive for both von Willebrand Factor (vWF) and thrombomodulin (the combined expression of which is limited to endothelium), and they stain for flt and flk. In recent testing, melanoma A2058, SKMEL.3 and A375.S2 are positive, while murine melanoma M3 is negative. One smooth muscle line (HISM) was positive and one negative (TIGHA-VSMC). Human blood lymphoctes are negative with or without prior stimulation. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse, Dog, Human
    • Immunogen

      Immunization of mice with HUVEC

    • Positive control

      • Human, mouse or dog blood or marrow cells Human intestinal smooth muscle cells Melanoma A2058, SKMEL.3 or A375.S2 cells Normal, primary HUVEC or MVEC cultures Human skin, intestine, ovary tonsil, lymph node, lung or kidney tissue.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.60
      Preservative: 0.1% Sodium azide
      Constituents: 1.5% BSA, 1.45% Sodium chloride, 0.0536% PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Monoclonal
    • Clone number

      P1H12
    • Isotype

      IgG1
    • Research areas

      • Immunology
      • Cell Type Markers
      • CD
      • Endothelial Cells
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cell Adhesion
      • Cell Adhesion Molecules
      • Endothelial
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Cell adhesion
      • Other
      • Stem Cells
      • Endothelial Progenitors
      • Endothelial Markers
      • Cardiovascular
      • Angiogenesis
      • Endothelial Cell Markers

    Associated products

    • Alternative Versions

      • Anti-CD146 antibody [P1H12] - BSA and Azide free (ab230295)
      • PE Anti-CD146 antibody [P1H12] (ab234280)
      • Anti-CD146 antibody [P1H12] (ab24577)
    • Isotype control

      • FITC Mouse IgG1 [B11/6] - Isotype Control (ab91356)
    • Recombinant Protein

      • Recombinant Human CD146 protein (ab114180)

    Applications

    Our Abpromise guarantee covers the use of ab78451 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 2µg for 106 cells.

    Methanol fixed cells.

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    Target

    • Function

      Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.
    • Tissue specificity

      Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.
    • Sequence similarities

      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 2 Ig-like V-type (immunoglobulin-like) domains.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P43121 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4162 Human
      • Entrez Gene: 84004 Mouse
      • Omim: 155735 Human
      • SwissProt: P43121 Human
      • SwissProt: Q8R2Y2 Mouse
      • Unigene: 599039 Human
      • Unigene: 275003 Mouse
      • Alternative names

        • A32 antigen antibody
        • CD 146 antibody
        • CD146 antibody
        • CD146 antigen antibody
        • Cell surface glycoprotein MUC18 antibody
        • Cell surface glycoprotein P1H12 antibody
        • Gicerin antibody
        • Mcam antibody
        • Melanoma adhesion molecule antibody
        • Melanoma associated antigen A32 antibody
        • Melanoma associated antigen MUC18 antibody
        • Melanoma associated glycoprotein MUC18 antibody
        • Melanoma cell adhesion molecule antibody
        • Melanoma-associated antigen A32 antibody
        • Melanoma-associated antigen MUC18 antibody
        • MelCAM antibody
        • MUC 18 antibody
        • MUC18 antibody
        • MUC18_HUMAN antibody
        • S endo 1 antibody
        • S endo 1 endothelial associated antigen antibody
        • S-endo 1 endothelial-associated antigen antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-CD146 antibody [P1H12] (ab78451)
        Flow Cytometry - FITC Anti-CD146 antibody [P1H12] (ab78451)
        Overlay histogram showing MCF-7 cells stained with ab78451 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78451, 2µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in MCF-7 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

        Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

      Protocols

      • Flow cytometry protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (5)

      Publishing research using ab78451? Please let us know so that we can cite the reference in this datasheet.

      ab78451 has been referenced in 5 publications.

      • Hu J  et al. Effects of menstrual blood-derived stem cells on endometrial injury repair. Mol Med Rep 19:813-820 (2019). PubMed: 30569163
      • Rao F  et al. Exosomes from Human Gingiva-Derived Mesenchymal Stem Cells Combined with Biodegradable Chitin Conduits Promote Rat Sciatic Nerve Regeneration. Stem Cells Int 2019:2546367 (2019). PubMed: 31191669
      • Parasar P  et al. Differentiating mouse embryonic stem cells express markers of human endometrium. Reprod Biol Endocrinol 15:52 (2017). PubMed: 28716123
      • Nemeth K  et al. Impaired function of bone marrow stromal cells in systemic mastocytosis. Stem Cell Res 15:42-53 (2015). Flow Cyt ; Human . PubMed: 26001169
      • Graham JJ  et al. Long-term tracking of bone marrow progenitor cells following intracoronary injection post-myocardial infarction in swine using MRI. Am J Physiol Heart Circ Physiol 299:H125-33 (2010). ICC/IF ; Pig . PubMed: 20418483

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab78451.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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