FITC Anti-CD146 antibody [P1H12] (ab78451)
![Flow Cytometry - FITC Anti-CD146 antibody [P1H12] (ab78451)](https://www.abcam.com/ps/products/78/ab78451/Images/ab78451-94261-anti-cd146-antibody-p1h12-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-CD146 antibody [P1H12] (ab78451)")
Key features and details
- FITC Mouse monoclonal [P1H12] to CD146
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-CD146 antibody [P1H12]
See all CD146 primary antibodies -
Description
FITC Mouse monoclonal [P1H12] to CD146 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab78451 reacts specifically with CD146. In blood and marrow ab78451 reacts only with endothelial cells, and has been used to detect circulating endothelial cells in human, dogs, rabbits and mouse samples. ab78451 positively stains normal, primary HUVEC and MVEC cultures and the endothelial cells of all vessels in normal frozen sections of human skin, intestine, ovary tonsil, lymph node, lung, and kidney. It does not stain carcinoma cell lines HT-29 and COLO205, and M21, the T cell lines Jurkat and HuT78, fibroblasts, HL-60 or CHO cells, EBV-transformed B cell lines, normal monocytes, granulocytes, red cells, platelets, T cells or B cells from marrow or peripheral blood; nor does it detect marrow megakaryocytes or the megakaryoblast line HU3. The peripheral blood cells that do stain with ab78451 are also positive for both von Willebrand Factor (vWF) and thrombomodulin (the combined expression of which is limited to endothelium), and they stain for flt and flk. In recent testing, melanoma A2058, SKMEL.3 and A375.S2 are positive, while murine melanoma M3 is negative. One smooth muscle line (HISM) was positive and one negative (TIGHA-VSMC). Human blood lymphoctes are negative with or without prior stimulation. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Immunization of mice with HUVEC
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Positive control
- Human, mouse or dog blood or marrow cells Human intestinal smooth muscle cells Melanoma A2058, SKMEL.3 or A375.S2 cells Normal, primary HUVEC or MVEC cultures Human skin, intestine, ovary tonsil, lymph node, lung or kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: 1.5% BSA, 1.45% Sodium chloride, 0.0536% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
P1H12 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 2µg for 106 cells.
Methanol fixed cells. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
Use 2µg for 106 cells. Methanol fixed cells. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration. -
Tissue specificity
Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4162 Human
- Omim: 155735 Human
- SwissProt: P43121 Human
- Unigene: 599039 Human
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Alternative names
- A32 antigen antibody
- CD 146 antibody
- CD146 antibody
see all
Images
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Flow Cytometry - FITC Anti-CD146 antibody [P1H12] (ab78451)Overlay histogram showing MCF-7 cells stained with ab78451 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78451, 2µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in MCF-7 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab78451 has been referenced in 7 publications.
- Evdokiou A et al. Characterization of Burn Eschar Pericytes. J Clin Med 9:N/A (2020). PubMed: 32102389
- Wu D et al. Long noncoding RNA TUG1 promotes osteogenic differentiation of human periodontal ligament stem cell through sponging microRNA-222-3p to negatively regulate Smad2/7. Arch Oral Biol 117:104814 (2020). PubMed: 32574885
- Hu J et al. Effects of menstrual blood-derived stem cells on endometrial injury repair. Mol Med Rep 19:813-820 (2019). PubMed: 30569163
- Rao F et al. Exosomes from Human Gingiva-Derived Mesenchymal Stem Cells Combined with Biodegradable Chitin Conduits Promote Rat Sciatic Nerve Regeneration. Stem Cells Int 2019:2546367 (2019). PubMed: 31191669
- Parasar P et al. Differentiating mouse embryonic stem cells express markers of human endometrium. Reprod Biol Endocrinol 15:52 (2017). PubMed: 28716123
- Nemeth K et al. Impaired function of bone marrow stromal cells in systemic mastocytosis. Stem Cell Res 15:42-53 (2015). Flow Cyt ; Human . PubMed: 26001169
- Graham JJ et al. Long-term tracking of bone marrow progenitor cells following intracoronary injection post-myocardial infarction in swine using MRI. Am J Physiol Heart Circ Physiol 299:H125-33 (2010). ICC/IF ; Pig . PubMed: 20418483