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  1. Link

    fitc-cd15-antibody-mem-158-ab18272.pdf

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FITC Anti-CD15 antibody [MEM-158] (ab18272)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)

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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • FITC Mouse monoclonal [MEM-158] to CD15
  • Suitable for: Flow Cyt
  • Reacts with: Species independent
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgM

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Overview

  • Product name

    FITC Anti-CD15 antibody [MEM-158]
    See all CD15 primary antibodies
  • Description

    FITC Mouse monoclonal [MEM-158] to CD15
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Tissue, cells or virus corresponding to Human CD15.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 8.0
    Preservative: 0.097% Sodium azide
    Constituents: PBS, BSA
  • Concentration information loading...
  • Purification notes

    Purified by precipitation and chromatography
  • Clonality

    Monoclonal
  • Clone number

    MEM-158
  • Isotype

    IgM
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Immunology
    • Cell Type Markers
    • CD
    • Adhesion
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Cancer
    • Tumor immunology
    • CD markers
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers

Associated products

  • Alternative Versions

    • PerCP/Cy5.5® Anti-CD15 antibody [MEM-158] (ab234266)
    • Biotin Anti-CD15 antibody [MEM-158] (ab28103)
    • Anti-CD15 antibody [MEM-158] (ab665)
    • APC Anti-CD15 antibody [MEM-158] (ab88032)
  • Isotype control

    • FITC Mouse IgM [B11/7] - Isotype Control (ab91546)

Applications

Our Abpromise guarantee covers the use of ab18272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Use 200 µl/ml whole blood.

 

 

 

ab91546 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
  • Cellular localization

    Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
  • Alternative names

    • 3 fucosyl N acetyl lactosamine antibody
    • 3-FAL antibody
    • 3-fucosyl-N-acetyl-lactosamine antibody
    • Leu M1 antibody
    • Lewis X antibody
    • LeX antibody
    • SSEA-1 antibody
    • stage-specific embryonic antigen 1 antibody
    see all

Protocols

  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab18272? Please let us know so that we can cite the reference in this datasheet.

    ab18272 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-CD15 antibody [MEM-158] (FITC)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Spleen)
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 23 2020

    Question

    Does this antibody react against the ssca1 antigen in addition to the Lewis x antigen?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 24 2006

    Answer

    Thank you for your enquiry. I hope that you receive our chromatin marketing material shortly. I mailed it to you yesterday. I have received the following response from the source of this antibody. "The CD15 antigen is expressed on immature bone marrow cells and leukemic cells of the myelo-monocytic lineage and sometimes on lymphocytic leukemia cells. ab18272 reacts with granulocytes and has virtually no reactivity against monocytes. I had tried to find all posssible informations in HLDA Workshop VI (WS code of ab18272 A053). I did not find any info about reactivity against ssca1 antigen. Thanks for understanding, I hope that this info will help you". I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Mar 31 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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