FITC Anti-CD16 antibody [KD1] (ab124042)

Submit a review Q&A (1)References (2)
Flow Cytometry - FITC Anti-CD16 antibody [KD1] (ab124042)

    Key features and details

    • FITC Mouse monoclonal [KD1] to CD16
    • Suitable for: Flow Cyt
    • Reacts with: Sheep, Horse, Cow, Human, Pig
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG2a

    Overview

    • Product name

      FITC Anti-CD16 antibody [KD1]
      See all CD16 primary antibodies

    • Description

      FITC Mouse monoclonal [KD1] to CD16

    • Host species

      Mouse

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    • Specificity

      A number of studies have demonstrated the clone KD1 can be used to identify CD16 in a range of species including bovine and ovine.
      ab124042 reacts weakly with Horse and Pig.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Sheep, Horse, Cow, Human, Pig
      Does not react with: Rat, Dog

    • Immunogen

      Human CD16

    • Positive control

      • Human peripheral blood lymphocytes

    • General notes

      This product should be stored undiluted.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab124042 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 10µl for 106 cells.

    ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

    • Tissue specificity

      Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.

    • Sequence similarities

      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

    • Post-translational
      modifications

      Glycosylated. Contains high mannose- and complex-type oligosaccharides.
      The soluble form is produced by a proteolytic cleavage.

    • Cellular localization

      Cell membrane. Secreted. Exists also as a soluble receptor.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 16 antibody
        • CD 16a antibody
        • CD16 antibody
        • CD16a antibody
        • CD16a antigen antibody
        • CD16B antibody
        • CD16b antigen antibody
        • Fc fragment of IgG antibody
        • Fc fragment of IgG low affinity IIIa receptor (CD16) antibody
        • Fc fragment of IgG low affinity IIIa receptor antibody
        • Fc fragment of IgG receptor IIIa antibody
        • Fc fragment of IgG, low affinity III, receptor (CD16) antibody
        • Fc fragment of IgG, low affinity III, receptor for (CD16) antibody
        • Fc fragment of IgG, low affinity IIIa, receptor (CD16) antibody
        • Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antibody
        • Fc fragment of IgG, low affinity IIIa, receptor for antibody
        • Fc fragment of IgG, low affinity IIIb, receptor (CD16b) antibody
        • Fc fragment of IgG, low affinity IIIb, receptor for (CD16) antibody
        • Fc gamma R3 antibody
        • Fc gamma receptor III 2 (CD 16) antibody
        • Fc gamma receptor III A antibody
        • Fc gamma receptor IIIA antibody
        • Fc gamma receptor IIIb (CD 16) antibody
        • Fc gamma RIII alpha antibody
        • Fc gamma RIII antibody
        • Fc gamma RIII beta antibody
        • Fc gamma RIIIa antibody
        • Fc gamma RIIIb antibody
        • Fc of IgG antibody
        • Fc-gamma receptor III2 (CD 16) antibody
        • Fc-gamma receptor III2 (CD16) antibody
        • Fc-gamma receptor IIIb (CD16) antibody
        • Fc-gamma RIII antibody
        • Fc-gamma RIII-alpha antibody
        • Fc-gamma RIIIa antibody
        • FCG 3 antibody
        • FCG3 antibody
        • FCG3A_HUMAN antibody
        • FCgammaRIIIA antibody
        • FCGR 3 antibody
        • FCGR 3A antibody
        • FCGR3 antibody
        • FCGR3A antibody
        • FCGR3A protein antibody
        • FCGRIII antibody
        • FCGRIII-2 antibody
        • FcR 10 antibody
        • FcR-10 antibody
        • FcR10 antibody
        • FcRIII antibody
        • FcRIIIa antibody
        • IGFR 3 antibody
        • IGFR3 antibody
        • IgG Fc receptor III 1 antibody
        • IgG Fc receptor III 2 antibody
        • IgG Fc receptor III-2 antibody
        • IMD20 antibody
        • immunoglobulin G Fc receptor III antibody
        • immunoglobulin G Fc receptor III-2 antibody
        • Low affinity IIIa receptor antibody
        • Low affinity immunoglobulin gamma Fc region receptor III A antibody
        • Low affinity immunoglobulin gamma Fc region receptor III-A antibody
        • Low affinity immunoglobulin gamma Fc region receptor IIIB antibody
        • neutrophil-specific antigen NA antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-CD16 antibody [KD1] (ab124042)
        Flow Cytometry - FITC Anti-CD16 antibody [KD1] (ab124042)
        ab124042, undiluted, staining CD16 in Human peripheral blood lymphocytes by Flow cytometry.

      References (2)

      Publishing research using ab124042? Please let us know so that we can cite the reference in this datasheet.

      ab124042 has been referenced in 2 publications.

      • Chen X  et al. Retinoic acid facilitates inactivated transmissible gastroenteritis virus induction of CD8(+) T-cell migration to the porcine gut. Sci Rep 6:24152 (2016). Flow Cyt . PubMed: 27080036
      • Elhmouzi-Younes J  et al. Ovine CD16+/CD14- blood lymphocytes present all the major characteristics of natural killer cells. Vet Res 41:4 (2010). PubMed: 19755096

      Customer reviews and Q&As

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      Question

      Dear Mr/ Mrs, I’m looking for the human CD16 antibody, clone KD1, IgG2A which was shown to cross-react with sheep. The attached paper of Elhmouzi-Younes et al. does not refer to Abcam, but when I googled clone KD1 I found you are providing it. Can you confirm that this antibody (ab124049) is the one described in these papers? If so, can you offer me a test vial? Elhmouzi-Younes J, Boysen P, Pende D, et al. Ovine CD16+/CD14- blood lymphocytes present all the major characteristics of natural killer cells. Veterinary research 2010;41:4. 2. Moretta A, Tambussi G, Ciccone E, Pende D, Melioli G, Moretta L. CD16 surface molecules regulate the cytolytic function of CD3CD16+ human natural killer cells. International journal of cancer Journal international du cancer 1989;44:727-30. Kind regards,

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      Answer

      Thank you for your enquiry and your interest in our products. This is to confirm that ab124049 recognizes CD16 and it detect with sheep CD16. The isotype is indeed the IgG2a and ab124049 has been tested and characterized for Flow cytometry. We are not aware of these specific publications. Since this clone is licensed, it may well be that several companies may sell clone KD1. For your information, we also sell the FITC-conjugated version (ab124042) of this parent antibody (ab124049) which allows direct immunostaining without applying a compatible secondary antibody. You may wish to take a look at the product datasheet of this labelled antibody as well. I hope this helps and if I can assist further, please do not hesitate to contact me.

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