FITC Anti-CD161 antibody [HP-3G10] (ab210285)
Key features and details
- FITC Mouse monoclonal [HP-3G10] to CD161
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-CD161 antibody [HP-3G10]
See all CD161 primary antibodies -
Description
FITC Mouse monoclonal [HP-3G10] to CD161 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Baboon, Rhesus monkey -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Human peripheral blood lymphocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.1% Gelatin, 0.12% Monobasic dihydrogen sodium phosphate -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab210285 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
HP-3G10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 5µl for 105-8 cells.
The antibody has been diluted for use at 5 µl (0.5 μg) per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 µl. |
Notes |
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Flow Cyt
Use 5µl for 105-8 cells. The antibody has been diluted for use at 5 µl (0.5 μg) per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 µl. |
Target
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Function
Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. -
Tissue specificity
Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype. -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsN-glycosylated. Contains sialic acid residues. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3820 Human
- Omim: 602890 Human
- SwissProt: Q12918 Human
- Unigene: 169824 Human
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Alternative names
- C-type lectin domain family 5 member B antibody
- CD161 antibody
- CLEC5B antibody
see all
Images
Datasheets and documents
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Datasheet download
References (0)
ab210285 has not yet been referenced specifically in any publications.