FITC Anti-CD177 antibody [MEM-166] (ab26013)

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Flow Cytometry - FITC Anti-CD177 antibody [MEM-166] (ab26013)

    Key features and details

    • FITC Mouse monoclonal [MEM-166] to CD177
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    Overview

    • Product name

      FITC Anti-CD177 antibody [MEM-166]
      See all CD177 primary antibodies

    • Description

      FITC Mouse monoclonal [MEM-166] to CD177

    • Host species

      Mouse

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    • Tested Applications & Species

      Application Species
      Flow Cyt
      Human
      See all applications and species data

    • Immunogen

      Human granulocytes.

    • General notes

      The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 7.4
      Preservative: 0.097% Sodium azide
      Constituents: PBS, 0.2% BSA
    • Concentration information loading...

    • Purity

      Size exclusion

    • Purification notes

      Purity >95% by SDS-PAGE.

    • Clonality

      Monoclonal

    • Clone number

      MEM-166

    • Isotype

      IgG1

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab26013 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    Flow Cyt
    Human
    All applications
    Non human primates
    Application Abreviews Notes
    Flow Cyt
    Use 20µl for 106 cells.

    or 100 µl of whole blood. (The antibody should be used undiluted with the samples).

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
    Notes
    Flow Cyt
    Use 20µl for 106 cells.

    or 100 µl of whole blood. (The antibody should be used undiluted with the samples).

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    Target

    • Tissue specificity

      Highly expressed in normal bone marrow and weakly expressed in fetal liver. Expressed on neutrophils. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET).

    • Sequence similarities

      Contains 2 UPAR/Ly6 domains.

    • Post-translational
      modifications

      N-glycosylated.
      A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding).

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD177 antibody
        • CD177 antigen antibody
        • CD177 molecule antibody
        • CD177_HUMAN antibody
        • HNA-2a antibody
        • HNA2A antibody
        • Human neutrophil alloantigen 2a antibody
        • NB1 antibody
        • NB1 glycoprotein antibody
        • NB1 GP antibody
        • Polycythemia rubra vera 1 antibody
        • Polycythemia rubra vera protein 1 antibody
        • PRV-1 antibody
        • PRV1 antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-CD177 antibody [MEM-166] (ab26013)
        Flow Cytometry - FITC Anti-CD177 antibody [MEM-166] (ab26013)

        Surface staining of human peripheral blood cells with anti-CD177.

        ab26013 was used at a recommended concentration of 1 μg/mL.

      References (2)

      Publishing research using ab26013? Please let us know so that we can cite the reference in this datasheet.

      ab26013 has been referenced in 2 publications.

      • Liu H  et al. Proteinase 3 expression on the neutrophils of patients with paroxysmal nocturnal hemoglobinuria. Exp Ther Med 15:2525-2532 (2018). PubMed: 29467851
      • Hu N  et al. Decreased CXCR1 and CXCR2 expression on neutrophils in anti-neutrophil cytoplasmic autoantibody-associated vasculitides potentially increases neutrophil adhesion and impairs migration. Arthritis Res Ther 13:R201 (2011). Flow Cyt ; Human . PubMed: 22152684

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Question

      Phone call: Customer has kindly requested the information about dilution of use in flow cytometry.

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      Answer

      Thank you very much for your phone call! I can confirm that the total volume of the product is 2ml which means the product should be used undiluted in Flow Cytometry. I hope this information will be helpful. Please do not hesitate to contact me if you have any question.

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      Question

      Does this antibody react with monocytes; it has reactivity with granulocytes.

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      Answer

      Thank you for your enquiry. Further to correspondence with the source of this enquiry I can tell you that whilst the immunogen of ab26013 was isolated human granulocytes the expression of CD177 has been described in monocytes. I would therefore predict that this antibody would recognise these cells. For example the expression of CD177 on monocytes has been doucmented during pregnancy and in certain diseases: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dop t=Abstract&list_uids=15043575&query_hl=8&itool=pubmed_docsum I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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