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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Beta
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FITC Anti-CD18 antibody [MEM-48], prediluted (ab18238)

  • Datasheet
  • SDS
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Flow Cytometry - FITC Anti-CD18 antibody [MEM-48], prediluted (ab18238)

    Key features and details

    • FITC Mouse monoclonal [MEM-48] to CD18, prediluted
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    You may also be interested in

    Protein
    Product image
    Recombinant Human CD18 protein (ab112311)

    View more associated products

    Overview

    • Product name

      FITC Anti-CD18 antibody [MEM-48], prediluted
      See all CD18 primary antibodies
    • Description

      FITC Mouse monoclonal [MEM-48] to CD18, prediluted
    • Host species

      Mouse
    • Conjugation

      FITC. Ex: 493nm, Em: 528nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Leucocytes of a patient suffering from a LGL-type leukaemia (Human).

    • Epitope

      The antibody recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituents: PBS, BSA
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      MEM-48
    • Isotype

      IgG1
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cell Adhesion
      • Integrins
      • Beta
      • Immunology
      • Cell Type Markers
      • CD
      • Adhesion
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • NK Cell Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Dendritic Cell Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Monocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Mast Cell Lineage

    Associated products

    • Alternative Versions

      • Biotin Anti-CD18 antibody [MEM-48] (ab28093)
      • APC Anti-CD18 antibody [MEM-48], prediluted (ab40914)
      • Anti-CD18 antibody [MEM-48] - Low endotoxin, Azide free (ab46700)
      • PE Anti-CD18 antibody [MEM-48] (ab49921)
      • Anti-CD18 antibody [MEM-48] (ab657)
    • Isotype control

      • FITC Mouse IgG1 [B11/6] - Isotype Control (ab91356)
    • Recombinant Protein

      • Recombinant Human CD18 protein (ab112311)

    Applications

    Our Abpromise guarantee covers the use of ab18238 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 0.75µl for 106 cells.

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
    • Involvement in disease

      Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
    • Sequence similarities

      Belongs to the integrin beta chain family.
      Contains 1 VWFA domain.
    • Post-translational
      modifications

      Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P05107 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3689 Human
      • Omim: 600065 Human
      • SwissProt: P05107 Human
      • Unigene: 375957 Human
      • Alternative names

        • 95 subunit beta antibody
        • Beta 2 (antigen CD18 (p95) antibody
        • CD 18 antibody
        • CD18 antibody
        • Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody
        • Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody
        • Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody
        • Complement component 3 receptor 3 and 4 subunit antibody
        • Complement receptor C3 beta subunit antibody
        • Complement receptor C3 subunit beta antibody
        • Integrin beta 2 antibody
        • Integrin beta chain beta 2 antibody
        • Integrin beta-2 (ITGB2) antibody
        • Integrin beta-2 antibody
        • Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody
        • ITB2_HUMAN antibody
        • ITGB2 antibody
        • LAD antibody
        • LCAMB antibody
        • Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody
        • Leukocyte cell adhesion molecule CD18 antibody
        • LFA 1 antibody
        • LFA1 antibody
        • Lymphocyte function associated antigen 1 (LFA1) antibody
        • Lymphocyte function associated antigen 1 antibody
        • MAC 1 antibody
        • MAC1 antibody
        • Macrophage antigen 1 (mac1) beta antibody
        • MF17 antibody
        • MFI7 antibody
        • OTTHUMP00000115278 antibody
        • OTTHUMP00000115279 antibody
        • OTTHUMP00000115280 antibody
        • OTTHUMP00000115281 antibody
        • OTTHUMP00000115282 antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-CD18 antibody [MEM-48], prediluted (ab18238)
        Flow Cytometry - FITC Anti-CD18 antibody [MEM-48], prediluted (ab18238)
        Overlay histogram showing peripheral blood lymphocytes stained with ab18238 (red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18238, 0.5 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (2 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab18238? Please let us know so that we can cite the reference in this datasheet.

      ab18238 has been referenced in 1 publication.

      • Yu T  et al. Affinity, lateral mobility, and clustering contribute independently to beta 2-integrin-mediated adhesion. Am J Physiol Cell Physiol 299:C399-410 (2010). PubMed: 20445173

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab18238.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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