FITC Anti-CD1d antibody [WTH1] (ab119897)

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Flow Cytometry - FITC Anti-CD1d antibody [WTH1] (ab119897)

    Key features and details

    • FITC Mouse monoclonal [WTH1] to CD1d
    • Suitable for: Flow Cyt
    • Reacts with: Mouse, Rat
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG2a

    Overview

    • Product name

      FITC Anti-CD1d antibody [WTH1]
      See all CD1d primary antibodies

    • Description

      FITC Mouse monoclonal [WTH1] to CD1d

    • Host species

      Mouse

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    • Specificity

      Binds with high affinity to the mature CD1d heavy chain independently of beta-2-microglobulin.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse, Rat

    • Immunogen

      Rat CD1d-transduced M12.4.1.C3 cells.

    • Positive control

      • Rat thymocytes.

    • General notes


      Clone WTH1 efficiently blocks rat and mouse invariant natural killer T cell (iNKT cell) response to a-GalCer (an antigen for iNKT cells).

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab119897 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 10µl for 106 cells.

    in 100 µl.

     

     

     

    ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.

    • Tissue specificity

      Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.

    • Sequence similarities

      Contains 1 Ig-like (immunoglobulin-like) domain.

    • Cellular localization

      Cell membrane. Endosome membrane. Lysosome membrane. Subject to intracellular trafficking between the cell membrane, endosomes and lysosomes.
    • Information by UniProt

    • Database links

      • Alternative names

        • Antigen-presenting glycoprotein CD1d antibody
        • CD1.1 antibody
        • CD1A antibody
        • CD1d antibody
        • CD1D antigen antibody
        • CD1D antigen d polypeptide antibody
        • CD1d molecule antibody
        • CD1D_HUMAN antibody
        • Cd1d1 antibody
        • differentiation antigen CD1 alpha 3 antibody
        • HMC class I antigen like glycoprotein CD1D antibody
        • Ly 38 antibody
        • MGC34622 antibody
        • R3 antibody
        • R3G1 antibody
        • T cell surface glycoprotein CD1d antibody
        • Thymocyte antigen CD1D antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-CD1d antibody [WTH1] (ab119897)
        Flow Cytometry - FITC Anti-CD1d antibody [WTH1] (ab119897)
        Staining of rat thymocytes with ab119897 at 10µl X 106 cells.

      References (1)

      Publishing research using ab119897? Please let us know so that we can cite the reference in this datasheet.

      ab119897 has been referenced in 1 publication.

      • Monzon-Casanova E  et al. CD1d expression in paneth cells and rat exocrine pancreas revealed by novel monoclonal antibodies which differentially affect NKT cell activation. PLoS One 5: (2010). PubMed: 20927351

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