FITC Anti-CD2 antibody [LT2] (ab27995)
![Flow Cytometry - FITC Anti-CD2 antibody [LT2] (ab27995)](https://www.abcam.com/ps/products/27/ab27995/Images/ab27995-38900-anti-cd2-antibody-lt2-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-CD2 antibody [LT2] (ab27995)")
Key features and details
- FITC Mouse monoclonal [LT2] to CD2
- Suitable for: Flow Cyt
- Reacts with: Human, Rhesus monkey
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Overview
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Product name
FITC Anti-CD2 antibody [LT2]
See all CD2 primary antibodies -
Description
FITC Mouse monoclonal [LT2] to CD2 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab27995 recognises CD2 expressed by T cells, NK cells and thymocytes. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human, Rhesus monkey -
Immunogen
Tissue/ cell preparation: Normal human peripheral blood lymphocytes.
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General notes
ab27995 inhibits NK cell mediated cytotoxicity, and ConA and PHA mediated blast transformation.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
ab27995 inhibits NK cell mediated cytotoxicity, and ConA and PHA mediated blast transformation. -
Clonality
Monoclonal -
Clone number
LT2 -
Myeloma
x63-Ag8.653 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab27995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. Use 10µl for 106 cells, or 100µl of whole blood.
ab91368 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|
Target
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Function
CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 914 Human
- Entrez Gene: 677855 Rhesus monkey
- Omim: 186990 Human
- SwissProt: P06729 Human
- Unigene: 523500 Human
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Alternative names
- CD 2 antibody
- CD2 antibody
- CD2 antigen (p50), sheep red blood cell receptor antibody
see all
Images
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Flow Cytometry - FITC Anti-CD2 antibody [LT2] (ab27995)FACS analysis of CD2 expression in human lymphocytes using FITC conjugated ab27995 at 10µl for 106 cells.
Datasheets and documents
References (2)
ab27995 has been referenced in 2 publications.
- Lin CW et al. CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood 106:3567-74 (2005). PubMed: 16046525
- Beyers AD et al. Activation of T lymphocytes via monoclonal antibodies against rat cell surface antigens with particular reference to CD2 antigen. Immunol Rev 111:59-77 (1989). PubMed: 2576418
