FITC Anti-CD2 antibody [LT2] (ab27995)
![Flow Cytometry - FITC Anti-CD2 antibody [LT2] (ab27995)](https://www.abcam.com/ps/products/27/ab27995/Images/ab27995-38900-anti-cd2-antibody-lt2-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-CD2 antibody [LT2] (ab27995)")
Key features and details
- FITC Mouse monoclonal [LT2] to CD2
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
FITC Anti-CD2 antibody [LT2]
See all CD2 primary antibodies -
Description
FITC Mouse monoclonal [LT2] to CD2 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab27995 recognises CD2 expressed by T cells, NK cells and thymocytes. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD2. Normal human peripheral blood lymphocytes.
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General notes
ab27995 inhibits NK cell mediated cytotoxicity, and ConA and PHA mediated blast transformation.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
ab27995 inhibits NK cell mediated cytotoxicity, and ConA and PHA mediated blast transformation. -
Clonality
Monoclonal -
Clone number
LT2 -
Myeloma
x63-Ag8.653 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 10µl for 106 cells.
Use 10µl for 106 cells, or 100µl of whole blood.
ab91368 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
Flow Cyt
Use 10µl for 106 cells. Use 10µl for 106 cells, or 100µl of whole blood.
ab91368 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|
Target
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Function
CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 914 Human
- Omim: 186990 Human
- SwissProt: P06729 Human
- Unigene: 523500 Human
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Alternative names
- CD 2 antibody
- CD2 antibody
- CD2 antigen (p50), sheep red blood cell receptor antibody
see all
Images
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Flow Cytometry - FITC Anti-CD2 antibody [LT2] (ab27995)FACS analysis of CD2 expression in human lymphocytes using FITC conjugated ab27995 at 10µl for 106 cells.
Datasheets and documents
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Datasheet download
References (2)
ab27995 has been referenced in 2 publications.
- Lin CW et al. CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood 106:3567-74 (2005). PubMed: 16046525
- Beyers AD et al. Activation of T lymphocytes via monoclonal antibodies against rat cell surface antigens with particular reference to CD2 antigen. Immunol Rev 111:59-77 (1989). PubMed: 2576418
