FITC Anti-CD226 antibody [DX11] (ab33335)

Submit a review Submit a question References (1)
Flow Cytometry - FITC Anti-CD226 antibody [DX11] (ab33335)

    Key features and details

    • FITC Mouse monoclonal [DX11] to CD226
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Biotin PE Unconjugated

    Overview

    • Product name

      FITC Anti-CD226 antibody [DX11]
      See all CD226 primary antibodies

    • Description

      FITC Mouse monoclonal [DX11] to CD226

    • Host species

      Mouse

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Tissue, cells or virus corresponding to Human CD226. Human cytotoxic T lymphocyte clone.

    • Positive control

      • Human peripheral blood lymphocytes.

    • General notes


      Clone DX11 is reported to inhibit T and NK cell mediated cytotoxicity against tumour cell targets and to block TNF alpha and IFN gamma secretion by alloantigen specific T cells.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 7.40
      Preservative: 0.09% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...

    • Purity

      Protein G purified

    • Purification notes

      This antibody was purified from tissue culture supernatant.

    • Primary antibody notes

      Clone DX11 is reported to inhibit T and NK cell mediated cytotoxicity against tumour cell targets and to block TNF alpha and IFN gamma secretion by alloantigen specific T cells.

    • Clonality

      Monoclonal

    • Clone number

      DX11

    • Myeloma

      Sp2/0

    • Isotype

      IgG1

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab33335 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    1/10 - 1/20.

    Use 10µl of the suggested working dilution to label 106 cells in 100µl.

     

     

     

     

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     
    Notes
    Flow Cyt
    1/10 - 1/20.

    Use 10µl of the suggested working dilution to label 106 cells in 100µl.

     

     

     

     

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell.

    • Tissue specificity

      Expressed by peripheral blood T-lymphocytes.

    • Sequence similarities

      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • adhesion glycoprotein antibody
        • CD226 antibody
        • CD226 antigen antibody
        • CD226 molecule antibody
        • CD226_HUMAN antibody
        • DNAM 1 antibody
        • DNAM-1 antibody
        • DNAM1 antibody
        • DNAX accessory molecule 1 antibody
        • platelet and T cell activation antigen 1 antibody
        • PTA1 antibody
        • T lineage specific activation antigen 1 antigen antibody
        • TLiSA1 antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-CD226 antibody [DX11] (ab33335)
        Flow Cytometry - FITC Anti-CD226 antibody [DX11] (ab33335)
        Ab33335, at a dilution of 10µl for labeling 106 cells in 100µl, staining CD226 in human peripheral blood lymphocytes by Flow Cytometry.

      References (1)

      Publishing research using ab33335? Please let us know so that we can cite the reference in this datasheet.

      ab33335 has been referenced in 1 publication.

      • Kojima H  et al. CD226 mediates platelet and megakaryocytic cell adhesion to vascular endothelial cells. J Biol Chem 278:36748-53 (2003). PubMed: 12847109

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab33335.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com