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    fitc-cd226-antibody-dx11-ab33335.pdf

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Immunology Adaptive Immunity T Cells Cytotoxic Cells
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FITC Anti-CD226 antibody [DX11] (ab33335)

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Flow Cytometry - FITC Anti-CD226 antibody [DX11] (ab33335)

    Key features and details

    • FITC Mouse monoclonal [DX11] to CD226
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

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    Protein
    Product image
    Recombinant Human CD226 protein (denatured) (ab140062)
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    Anti-CD226 antibody [EPR21172] - BSA and Azide free (ab230865)

    View more associated products

    Overview

    • Product name

      FITC Anti-CD226 antibody [DX11]
      See all CD226 primary antibodies
    • Description

      FITC Mouse monoclonal [DX11] to CD226
    • Host species

      Mouse
    • Conjugation

      FITC. Ex: 493nm, Em: 528nm
    • Specificity

      This antibody recognises CD226.
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Tissue/ cell preparation: Human cytotoxic T lymphocyte clone.

    • Positive control

      • Human peripheral blood lymphocytes.
    • General notes


      Clone DX11 is reported to inhibit T and NK cell mediated cytotoxicity against tumour cell targets and to block TNF alpha and IFN gamma secretion by alloantigen specific T cells.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.40
      Preservative: 0.09% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      This antibody was purified from tissue culture supernatant.
    • Primary antibody notes

      Clone DX11 is reported to inhibit T and NK cell mediated cytotoxicity against tumour cell targets and to block TNF alpha and IFN gamma secretion by alloantigen specific T cells.
    • Clonality

      Monoclonal
    • Clone number

      DX11
    • Myeloma

      Sp2/0
    • Isotype

      IgG1
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • Cytotoxic Cells
      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Immunology
      • Cell Type Markers
      • CD
      • Adhesion

    Associated products

    • Alternative Versions

      • PE Anti-CD226 antibody [DX11] (ab33337)
      • Biotin Anti-CD226 antibody [DX11] (ab33393)
      • Anti-CD226 antibody [DX11] (ab33397)
    • Isotype control

      • FITC Mouse IgG1 [B11/6] - Isotype Control (ab91356)
    • Recombinant Protein

      • Recombinant Human CD226 protein (denatured) (ab140062)

    Applications

    Our Abpromise guarantee covers the use of ab33335 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt 1/10 - 1/20.

    Use 10µl of the suggested working dilution to label 106 cells in 100µl.

     

     

     

     

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell.
    • Tissue specificity

      Expressed by peripheral blood T-lymphocytes.
    • Sequence similarities

      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession Q15762 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10666 Human
      • Omim: 605397 Human
      • SwissProt: Q15762 Human
      • Unigene: 660130 Human
      • Alternative names

        • adhesion glycoprotein antibody
        • CD226 antibody
        • CD226 antigen antibody
        • CD226 molecule antibody
        • CD226_HUMAN antibody
        • DNAM 1 antibody
        • DNAM-1 antibody
        • DNAM1 antibody
        • DNAX accessory molecule 1 antibody
        • platelet and T cell activation antigen 1 antibody
        • PTA1 antibody
        • T lineage specific activation antigen 1 antigen antibody
        • TLiSA1 antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-CD226 antibody [DX11] (ab33335)
        Flow Cytometry - FITC Anti-CD226 antibody [DX11] (ab33335)
        Ab33335, at a dilution of 10µl for labeling 106 cells in 100µl, staining CD226 in human peripheral blood lymphocytes by Flow Cytometry.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab33335? Please let us know so that we can cite the reference in this datasheet.

      ab33335 has been referenced in 1 publication.

      • Kojima H  et al. CD226 mediates platelet and megakaryocytic cell adhesion to vascular endothelial cells. J Biol Chem 278:36748-53 (2003). PubMed: 12847109

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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