FITC Anti-CD24 antibody [SN3] (ab30350)

Reviews (1)Q&A (3)References (13)
Flow Cytometry - FITC Anti-CD24 antibody [SN3] (ab30350)

    Key features and details

    • FITC Mouse monoclonal [SN3] to CD24
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    Overview

    • Product name

      FITC Anti-CD24 antibody [SN3]
      See all CD24 primary antibodies

    • Description

      FITC Mouse monoclonal [SN3] to CD24

    • Host species

      Mouse

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Glycoproteins purified from human NALM-1 cell line

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Applications

    Our Abpromise guarantee covers the use of ab30350 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 20µl for 106 cells.

    1/1 - 1/10. Use 10µl of the above dilution for 106 cells or 100ul whole blood.

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    Target

    • Function

      Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.

    • Tissue specificity

      B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.

    • Involvement in disease

      Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS) [MIM:126200]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface.

    • Sequence similarities

      Belongs to the CD24 family.

    • Post-translational
      modifications

      Extensively O-glycosylated.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 24 antibody
        • CD24 antibody
        • CD24 antigen (small cell lung carcinoma cluster 4 antigen) antibody
        • CD24 antigen antibody
        • CD24 molecule antibody
        • CD24_HUMAN antibody
        • CD24A antibody
        • FLJ22950 antibody
        • FLJ43543 antibody
        • GPI linked surface mucin antibody
        • Heat stable antigen antibody
        • HSA antibody
        • MGC75043 antibody
        • Nectadrin antibody
        • Signal transducer CD24 antibody
        • Small cell lung carcinoma cluster 4 antigen antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-CD24 antibody [SN3] (ab30350)
        Flow Cytometry - FITC Anti-CD24 antibody [SN3] (ab30350)
        Staining of human peripheral blood granulocytes with SN3 mouse monoclonal anti-human CD24 FITC conjugated (ab30350)

      References (13)

      Publishing research using ab30350? Please let us know so that we can cite the reference in this datasheet.

      ab30350 has been referenced in 13 publications.

      • Huang N  et al. Neuroprotective effect of tanshinone IIA-incubated mesenchymal stem cells on Aß25-35-induced neuroinflammation. Behav Brain Res 365:48-55 (2019). PubMed: 30831140
      • Feng L  et al. Identification of new cancer stem cell markers and signaling pathways in HER-2-positive breast cancer by transcriptome sequencing. Int J Oncol 55:1003-1018 (2019). PubMed: 31545416
      • Li XY  et al. Doxorubicin resistance induces IL6 activation in the colon cancer cell line LS180. Oncol Lett 16:5923-5929 (2018). PubMed: 30344742
      • Wang L  et al. Gemcitabine treatment induces endoplasmic reticular (ER) stress and subsequently upregulates urokinase plasminogen activator (uPA) to block mitochondrial-dependent apoptosis in Panc-1 cancer stem-like cells (CSCs). PLoS One 12:e0184110 (2017). PubMed: 28854261
      • Leung CO  et al. Sox9 confers stemness properties in hepatocellular carcinoma through Frizzled-7 mediated Wnt/ß-catenin signaling. Oncotarget 7:29371-86 (2016). Human . PubMed: 27105493
      View all Publications for this product

      Customer reviews and Q&As

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      1-4 of 4 Abreviews or Q&A

      Question

                          Hi,   Our customer would like to know if the following antibodies are of IgG1, kappa  or lambda light chains (Information on datasheet only stated Isotypes IgG1/ IgM/ IgG2a)?   Item                                      Format                 Cat # Anti-CD200 antibody      unconjugated    ab17225 Anti-CD146 antibody      PE                           ab78488 Anti-CD34 antibody         unconjugated    ab6330 Anti-CD71 antibody         unconjugated    ab117720 Anti-CD24 antibody         FITC                       ab30350 Anti-CD73 antibody         unconjugated    ab81720                                                 PE                           ab106697 Anti-CD105 antibody      PE                           ab91238                                                 Unconjugated   ab11414 Anti-Cd45 antibody         PE                           ab90960 Anti-STRO1 antibody      unconjugated    ab102969 Anti-CD90 antibody         unconjugated    ab23894                                                 FITC                       ab11155 Anti-CD14 antibody         FITC                       ab86896               Anti-cytokeratin 15         unconjugated    ab1385 IgG2a Isotype control     FITC                       ab81197   Looking forward to your soonest reply. Thank you.   Best regards, Penny Hor BITA LIFESCIENCE SDN. BHD. C-23-05, Dataran 32, No. 2, Jalan 19/1, 46300 Petaling Jaya, Selangor Darul Ehsan, Malaysia t +603 7960 8286    e info@bitalifesc.com.my f +603 7960 8262     

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      Answer

      Thank you for contacting us. In general, the immunoglobulin class (IgG1, IgG2a, IgGM, etc.) and light chain sub-class (kappa or lambda) is listed on the online datasheet. When the light chain is not listed, then the light chain sub-class has not been determined. In these instances, it is probably that the light chain sub-class is kappa, given that murine serum immunoglobulins are 95% kappa and 5% lambda, as discussed in the reference at the following link: http://www.ncbi.nlm.nih.gov/pubmed/3116408 Mol Immunol. 1987 Jul;24(7):751-7. PMID:3116408 Of the antibodies your customer has enquired about, The light chain sub-class has been determined for three: ab90960, ab86896, and ab1385. All are kappa light chain. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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      Question

      I have used ab30350 in the past and it worked well in ICC. The current lot, GR30681-1 does not stain cell as expected. The previous lot stained the plasma membrane but the current lot stains the nucleus, which is not where CD24 is expressed. I am staining the same samples as before.

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      Answer

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab30350 with the order number 946525. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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      Immunocytochemistry/ Immunofluorescence abreview for Anti-CD24 antibody [SN3] (FITC)

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Pancreatic adenocarcinoma)
      Specification
      Pancreatic adenocarcinoma
      Fixative
      Paraformaldehyde
      Permeabilization
      No
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 24°C
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      Dr. Jennifer Dembinski

      Verified customer

      Submitted Nov 30 2009

      Question

      We are interested in doing some double immunofluorescent staining of cells for CD44 and CD24. As they are both membrane located we need to go the Fluorescent route. The cells are human and fresh from biopsy samples. There is also a possibility of applying this to FFPE tissue. Can you advise on the best combination of these two Ab's to use?

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      Answer

      Thank you for your enquiry. We do not have a CD44 antibody that is directly flurophore labelled that has been shown to work by BOTH IHC-Fr and IHC-P. However, we do have the following unconjugated antibody shown to work by both applications: ab23844 CD44 antibody [MAC329] Rat Monoclonal FACS, IHC-Fr, IHC-P, IP. We have the follwing antibodies that are fluorphore conjugated but only shown to work in IHC-Fr: ab19618 CD44 antibody [IM7] Phycoerythrin conjugated Rat Monoclonal FACS, IHC-Fr, IP. ab19622 CD44 antibody [IM7] FITC conjugated Rat Monoclonal FACS, IHC-Fr, IP. With respect to CD24; again we do not have an antibody that has been shown to work by both IHC-P and IHC-Fr. However, we do have directly conjugated antibodies that have been shown to work by IHC-Fr. They are as follows: ab25657 CD24 antibody [91] FITC conjugated Rat Monoclonal FACS, IHC-Fr, IP ab25281 CD24 antibody [91] PE/Cy5 conjugated Rat Monoclonal FACS, IHC-Fr, IP ab25646 CD24 antibody [91] Phycoerythrin conjugated Rat Monoclonal FACS, IHC-Fr, IP I consider your best option using a combination of the rat monoclonal antibodies according to the wavelengths that you can detect with your fluorescence microscope. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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