FITC Anti-CD28 antibody [CD28.2] (ab134472)
![Flow Cytometry - FITC Anti-CD28 antibody [CD28.2] (ab134472)](https://www.abcam.com/ps/products/134/ab134472/Images/ab134472-277215-anti-cd28-antibody-cd282-fitc-flow-cytometry.png "Flow Cytometry - FITC Anti-CD28 antibody [CD28.2] (ab134472)")
Key features and details
- FITC Mouse monoclonal [CD28.2] to CD28
- Suitable for: Flow Cyt
- Reacts with: Human, Non human primates
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-CD28 antibody [CD28.2]
See all CD28 primary antibodies -
Description
FITC Mouse monoclonal [CD28.2] to CD28 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human, Non human primates -
Immunogen
DC28.1.3.3 hybridoma cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
CD28.2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PE Anti-CD28 antibody [CD28.2], prediluted (ab134418)
- APC Anti-CD28 antibody [CD28.2], prediluted (ab155372)
- PE/Cy7® Anti-CD28 antibody [CD28.2], prediluted (ab155387)
- PerCP/Cy5.5® Anti-CD28 antibody [CD28.2], prediluted (ab157318)
- FITC Anti-CD28 antibody [CD28.2] (ab234227)
- PE/Cy7® Anti-CD28 antibody [CD28.2] (ab234276)
- Biotin Anti-CD28 antibody [CD28.2] (ab267385)
- APC/Cy7® Anti-CD28 antibody [CD28.2] (ab270676)
- Anti-CD28 antibody [CD28.2] - Low endotoxin, Azide free (ab85986)
- PerCP Anti-CD28 antibody [CD28.2] (ab88034)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab134472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. -
Tissue specificity
Expressed in T-cells and plasma cells, but not in less mature B-cells. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 940 Human
- Omim: 186760 Human
- SwissProt: P10747 Human
- Unigene: 591629 Human
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Alternative names
- CD 28 antibody
- CD28 antibody
- CD28 antigen antibody
see all
Images
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Flow Cytometry - FITC Anti-CD28 antibody [CD28.2] (ab134472)
Flow Cytometry analysis of human peripheral blood lymphocytes labeling CD28 with ab134472 at uL (0.5 µg) (solid line) or Mouse IgG1 (FITC) isotype control at 0.5 µg (dashed line).
References (0)
ab134472 has not yet been referenced specifically in any publications.
